NUTRITIONAL AND THERAPEUTIC EVALUATION OF SPIRULINA PLATENSIS

  • SACHIN KULKARNI Department of Biotechnology, Rajarshi Shahu College, Latur, Maharashtra, India.
  • DEEPALI CHAVAN Department of Biotechnology, Rajarshi Shahu College, Latur, Maharashtra, India.

Abstract

Objective: The present investigation was to isolate and produce Spirulina platensis on high scale for food pharmaceutical and aquaculture due to the presence of high protein content.


Methods: Cultivation of Spirulina preparation of culture medium, inoculums build up, growth monitoring, harvesting, drying, procedure for protein estimation, sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and DNA isolation of Spirulina platensis.


Results: The study shows that protein content of S. platensis was found to be 62% protein profile was studied through SDS-PAGE, the bands were in the range of 35 kDa–44 kDa. S. platensis contain high molecular weight DNA. The bands was seen and visualize under the transilluminator it reveals that the alga S. platensis contain high molecular weight DNA.


Conclusion: The production of Spirulina by simple pH determination method suggests economic production of alga by a simple process. The DNA isolation showing that these algae contain high molecular weight DNA. However, utilization of this biomass for varied end uses, such as food, feed, aquaculture, and pharmaceuticals.

Keywords: Spirulina, Harvesting, Sulfate polyacrylamide gel, Protein estimation DNA isolation

Author Biography

DEEPALI CHAVAN, Department of Biotechnology, Rajarshi Shahu College, Latur, Maharashtra, India.

Pharmaceutical Biotechnology working as lecturer one of good college in shivaji university

References

1. Venaktaraman LV, Singh N. Status of Research on Leaf Protein and Microalgae in India. Mysore, India: CFTRI; 1982.
2. Venkataraman LV, Becker EW. Biotechnology and Utilization of Algae-the India Experience. Mysore, India: Central Food Technological Research Institute; 1985. p. 25.
3. Henkrison R. Microalga Spirulina, superalimento del futuro. In: Ronore Enterprises. 2nd ed. España: Ediciones Urano, Barcelona; 1994. p. 222.
4. Venkataraman LV, Somasekaran T, Becker EW. Replacement value of blue-green alga (Spirulina platensis) for fishmeal and a vitamin-mineral premix for broiler chicks. Br Poult Sci 1994;35:373-81.
5. Belay A. Mass culture of Spirulina outdoors. The earthrise farms experience. In: Vonshak A, editor. Spirulina platensis (Arthrospira): Physiology, Cell-biology and Biotechnology. London: Taylor and Francis; 1997. p. 131-58.
6. Othes S, Pire R. Fatty acid composition of Chlorella and Spirulina microalgae species. J Aoac Int 2001;84:1708-14.
7. Ciferri O. Spirulina, the edible microorganism. Microbiol Rev 1983;47:551-78.
8. Ciferri O, Tiboni O. The biochemistry and industrial potential of Spirulina. Ann Rev Microbiol 1985;39:503-26.
9. Ayehunie S, Belay A, Baba A, Ruprecht RM. Inhibition of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis). J Acquir Immune Defic Syndr Hum Retrovirol 1998;18:7-12.
10. Dillon JC, Phan PA. Spirulina as a source of proteins in human nutrition. In: Doumengue F, Durand-Chastel H, Toulemont A, editors. Spiruline Algue de vie. Vol. 12. Monaco: Musée Océanographique, Bulletin de l´Institut Océanographique Monaco; 1993. p. 103-7.
11. Li ZY, Li YG, Guo SY, Li L, Zhang SL, Hirahashi T, et al. Spirulina platensis is a cyanobacterial species that is surmised to potentiate. Int Immunopharmacol 2002;2:423-34.
12. Hirahashi T, Matsumoto M, Hazeki K, Saeki Y, Ui M, Seya T. Activation of the human innate immune system by Spirulina: Augmentation of interferon production and NK cytotoxicity by oral administration of hot water extract of Spirulina platensis. Int Immunopharmacol 2002;2:423-34.
13. Chamorro G, Salazar M, Favila L, Bourges H. Pharmacology and toxicology of Spirulina alga. Rev Invest Clin 1996;48:389-99.
14. Kerby N, Rowell P. Potential and commercial applications for photosynthetic prokaryotes. In: Mann N, Carr N, editors. Photosynthetic Prokaryotes. New York: Plenum Press; 1992. p. 93-120.
15. Patnaik S, Sarkar R, Mitra A. Alginate immobilization of Spirulina platensis for wastewater treatment. Indian J Exp Biol 2001;39:824-6.
16. Rao SR, Sarada R, Ravishankar GA. Phycocyanin, a new elicitor for capsaicin and anthocyanin accumulation in plant cell cultures. Appl Microbiol Biotechnol 1996;46:619-21.
17. Bhat VB, Madyastha KM. Scavenging of peroxynitrite by phycocyanin and phycocya-nobilin from Spirulina platensis: Protection against oxidative damage to DNA. Bio-chemical and biophysical research communication. Biochem Biophys Res Commun 2001;285:262.
18. Shekharam K, Ventakaraman L, Salimath P. Carbohydrate composition and characterization of two unusual sugars from the blue-green algae Spirulina platensis. Phytochem 1987;26:2267-9.
19. Pugh N, Ross SA, Elsohly HN, Elsohly MA, Pasco DS. Isolation of three weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, Aphanizomenon flos-aguae and Chlorella pyrenoidosa. Planta Med 2001;67:737-42.
20. Zarrouk C. Contribution à L´Étude D´une Cyanophycée Influence de Divers Facteurs Physiques et Chimiques sur la Croissance et la Photosynthèse de Spirulina Maxima (Setch et Gardner) Geitler. Paris: University de Paris; 1966.
21. Borowitzka M. Algal growth media and sources of algal cultures. In: Borowitzka M, Borowitzka L, editors. Microalgal Biotechnology. Great Britain: Cambridge University Press; 1992. p. 456-65.
22. Centre of Science for Villages. Ecological Science and Technology Department. Dattapur, Wardha: Centre of Science for Villages; 2014.
23. Sudha SS, Karthic R, Rengaramunjan J, Athulya. Antimicrobial activity of Spirulina platensis and aphanothece sp. On selected clinical bacterial isolates and its antioxidant activity. South As J Biol Sci 2011;1:87-98.
24. Henrikson R. Spirulina: Health Discoveries from the Source of Life. United Kingdom: Positive Health Online; 1998.
25. Pelizer LH. Spirulina platensis is a cyanobacterium that has a high protein content and in Electronic J Biotechnol 2002;5:121.
26. Yamanaka G, Glazer AN. Dynamic aspects of phycobilisome structure. Arch Microbiol 1980;124:39-47.
27. Vachhani AK, Vonshak A. Genetics of Spirulina. In: Effects of Mg2+ on the growth and DNase activity of Spirulina platensis. United States: Taylor and Francis; 1997.
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KULKARNI, S., and D. CHAVAN. “NUTRITIONAL AND THERAPEUTIC EVALUATION OF SPIRULINA PLATENSIS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 7, Apr. 2020, pp. 86-90, doi:10.22159/ajpcr.2020.v13i7.34123.
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