PHYTOCHEMICAL EVALUATION, GC-MS ANALYSIS OF PHYTOACTIVE COMPOUNDS, AND ANTIBACTERIAL ACTIVITY STUDIES FROM LINUM USITATISSIMUM
Objective: The flaxseed or linseed (Linum usitatissimum) is a minor oil seed belonging to the family Linaceae and genus Linum, which has been used as a precious nutritional food grain and folklore medicine in human diets for thousands of years and more. Recently, it has been used as a source of nutraceuticals and identified as a healthy component whose benefits on health are generally attributed to the high concentration of linolenic acids (omega 3) and lignans as well as significant quantities of dietary fiber including soluble and insoluble fibers.
Methods: The present study was carried out to assess the various phytochemical compositions, Gas chromatogram and Mass spectrometry (GC-MS) analysis, and antibacterial potential of different extracts of L. usitatissimum.
Results: The crude extracts of L. usitatissimum revealed the presence of several biologically active phytochemicals with the highest quantity of steroids, terpenoids, flavonoids, tannins, cardiac glycosides, protein, and amino acids. The antibacterial potency was explored against pathogenic bacteria, and the highest inhibitory activity of ethanol and methanol extracts was obtained against Staphylococcus aureus. The GC-MS analysis provides different peaks determining the presence of 10 phytochemical compounds with different therapeutic activities. The phytoactive principles were described with their molecular formula, retention time, molecular weight, and peak area (%).
Conclusion: Our results confer the utility of this plant extract in developing novel broad-spectrum therapeutic agent.
2. Sangeetha K, Senthilkumar G, Pannerselvam A, Priya NS. Cultivation of oyster mushroom (Pleurotus sp) using different substrates and evaluate their potential of antibacterial and phytochemicals. Int J Res Pharm Sci 2018;10:997-1001.
3. Thamilmaraiselvi B, Francis PS. Investigation of phytochemical constituents in Spirulina fusiformis for antibacterial activity. Nati J Physiol Pharm Pharmacol 2018;8:1491-5.
4. Priya NS, Thamilmaraiselvi B, Francis SF, Sangeetha K. Investigation of phytochemical constituents in Azolla microphylla for antibacterial activity. Nati J Physiol Pharm Pharmacol 2018;8:1500-4.
5. Sasikala K, Mohan C. Total phenolic, flavanoid contents and GC-MS analysis of Canthium coromandelicum leaves extract. Int J Pharm Pharm Sci 2014;6:379-81.
6. Ponnamma SU, Manjunath K. GC-Ms analysis of phyto components in the methanolic extract of Justicia wynaadensis (Nees) T. Anders. Int J Pharm Bio Sci 2012;3:570-6.
7. Al-Bayati FA. Antibacterial activity of Linum usitatissimum L. seeds and active compound detection. Rafidain J Sci 2007;18:27-36.
8. Derwich E, Benziane Z, Chabir R, Taouil R. In vitro antibacterial activity and GC/MS analysis of the essential oil extract of leaves of Rosmarinus officinalis grown in Morocco elhoussine. Int J Pharm Pharm Sci 2011;3:89-95.
9. Kundu A, Saha S. In vitro antifungal activity of novel picolinamides against soil borne fungi and structure activity relationship. Plant Pathol J 2014;13:152-9.
10. Sun R, Lü M, Chen L, Li Q, Song H, Fuchun BI, et al. Design, synthesis, bioactivity, and structure-activity relationship (SAR) studies of novel benzoylphenylureas containing oxime ether group. J Agric Food Chem 2008;56:11376-91.
11. Thomas AN, Riazanskaia S, Cheung W, Xu Y, Goodacre R, Thomas CL, et al. Novel noninvasive identification of biomarkers by analytical profiling of chronic wounds using volatile organic compounds. Wound Repair Regen 2010;18:391-400.
12. Altameme HJ, Hameed IM, Abu-Serag NA. Analysis of bioactive phytochemical compounds of two medicinal plants Equisetum arvense and Alchemila vulgaris seeds using gas-chromatography-mass spectrometry and Fourier-transform infrared spectroscopy. Malays Appl Biol 2015;44:47-58.
13. Kumaradevan G, Damodaran R, Mani P, Dineshkumar G, Jayaseelan T. Phytochemical screening and GC-MS analysis of bioactive components of ethanol leaves extract of Clerodendrum Phlomidis (L.). Am J Biol Pharm Res 2015;2:142-8.
14. Allen F, Pon A, Greiner R, Wishart D. Computational prediction of electron ionization mass spectra to assist in GC/MS compound identification. Anal Chem 2016;88:7689-97.
15. Halliwell B, Gutteridge JM, Cross CE. Free radicals, antioxidants, and human disease: Where are we now? J Lab Clin Med 1992;119:598-620.
16. Narender BR, Tejaswini S, Sarika M, Karuna N, Shirisha R, Priyanka S. Antibacterial and antifungal activities of Linum usitatissimum (flax seeds). Int J Pharm Educ Res 2016;3:4-8.
17. Kanthal LK, Dey A, Satyavathi K, Bhojaraju P. GC-MS analysis of bio-active compounds in methanolic extract of lactuca runcinata DC. Pharmacogn Res 2014;6:58-61
18. Cherian S, Augusti KT. Antidiabetic effects of a glycoside of leucopelargonidin isolated from Ficus bengalensis linn. Indian J Exp Biol 1993;31:26-9.
19. Dangoggo SM, Faruq UZ, Hassan LG. Preliminary chemical and antibacterial activity of Pergularia tomentosa. Sokoto J Vet Sci 2002;4:8-11.
20. Hussain S, Anjum FM, Butt MS, Sheikh MA. Chemical composition and functional properties of flaxseed (Linum usitatissimum) flour. Sarhad J Agric 2008;24:649-53.
21. Rahmat D, Kumala S, Farida Y, Chandra, B. Antibacterial activity of ethanolic extract of Javanese turmeric rhizome entrappted in nanoparticles: A novel rule of chitosan.Int J Appl Pharm 2018;10:298-302.
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