SPECTROPHOTOMETRIC DETERMINATION OF TOTAL PHENOLIC CONTENT FOR STANDARDIZATION OF VARIOUS PHYLLANTHUS SPECIES

  • Rakhi Khabiya School of Pharmacy, Devi Ahilya Vishwa Vidhyalaya, Indore, Madhya Pradesh, India

Abstract

Objective: This study aims at comparison of total phenolic content of ethanolic extract of various species of Phyllanthus available in Gujarat state by spectrophotometric method.


Methods : The total phenolic content was determined quantitatively using the Folin Ciocalteu reagent, with Gallic acid as standard.


Results: The total phenolic content in methanolic extract of various Phyllanthus species  ranged from 41.801 to 87.542 mg/g of dry weight of extract, expressed as gallic acid equivalents. Phyllanthus urinaria was found to have highest phenolic content and Phyllanthus acidus  was found to have lowest phenolic content .


Conclusion: The total phenolic content will be helpful for developing new drugs and standardizing the various Phyllanthus species particularly morphological similar species. The presence of a high total phenolic content shows that the alcoholic extract of Phyllanthus may possess antioxidant properties, which could lead to a new field of research in the future.

Keywords: Phyllanthus, total phenolic content, gallic acid, spectrophotometric method
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How to Cite
Rakhi Khabiya. “SPECTROPHOTOMETRIC DETERMINATION OF TOTAL PHENOLIC CONTENT FOR STANDARDIZATION OF VARIOUS PHYLLANTHUS SPECIES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 8, July 2019, pp. 297-01, https://innovareacademics.in/journals/index.php/ajpcr/article/view/34323.
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Original Article(s)