• HIBU WAHID Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.
  • ANJUVAN SINGH Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.


Objective: Hydroxyapatite (Hap) is an important material used for implants because of its structural similarity with calcium phosphate of the teeth and bones. Hap is mostly used in the situations where load-bearing implants are needed, such as in dental and orthopedic applications. Dental plaque is caused due to the bacteria which induces secondary caries through microleakage between the restoration and the tooth structure.

Methods: An attempt has been made to provide materials with antibacterial activity by incorporating an antibacterial agent into the chemical mixture (Hap). This study delineates the process of synthesizing silver-doped Hap (Ca10Agx(PO4)6(OH)2) by wet chemical precipitation method and by varying the silver concentration (0.3%–0.5%). The synthesized silver-doped Hap was further characterized using Fourier transform infrared spectroscopy and X-ray diffraction (XRD) analysis. The antimicrobial activities were tested against Gram-negative bacteria Escherichia coli.

Results: The maximum zone of inhibition against Escherichia coli was observed when equal concentration of Hydroxyapatite and Silver were mixed together i.e XAg=0.5% Zone of inhibition helps to determine the antimicrobial activity of silver-doped Hap. An increase in the zone of inhibition indicates the least growth of microorganisms which was observed at a silver concentration XAg=0.5%.

Conclusions: Silver-doped Hap was synthesized successfully using the chemical precipitation method and was characterized using Fourier transform infrared spectroscopy and XRD analysis. It can be concluded that silver-doped Hap can be an excellent substitute material used as dental implant device.

Keywords: Hydroxyapatite, Antibacterial activity, Bioactivity, Biocompatibility

Author Biography

HIBU WAHID, Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.

Associate Professor, Department of biotechnology, LPU


1. Ciobanu CS, Iconaru SL, Chifiriuc MC, Costescu A, Le Coustumer P, Predoi D. Synthesis and antimicrobial activity of silver-doped hydroxyapatite nanoparticles. Biomed Res Int 2013;2013:916218.
2. Xin R, Leng Y, Chen J, Zhang Q. A comparative study of calcium phosphate formation on bioceramics in vitro and in vivo. Biomaterials 2005;26:6477-86.
3. Rámila A, Vallet-Regí M. Static and dynamic in vitro study of a sol-gel glass bioactivity. Biomaterials 2001;22:2301-6.
4. Ragel CV, Vallet-Regi M, Rodriguez-Lorenzo LM. Preparation and in vitro bioactivity of hydroxyapatite/solgel glass biphasic material. Biomaterials 2002;23:1865-72.
5. Poca L, Dubnika A, Loca D, Berzina-Cimdina L. Bioactivity of silver doped hydroxyapatite scaffolds in simulated body fluids. Key Eng Mater 2014;604:175-9.
6. Catauro M, Raucci MG, De Gaetano F, Marotta A. Antibacterial and bioactive silver-containing na2O x caO x 2SiO2 glass prepared by sol-gel method. J Mater Sci Mater Med 2004;15:831-7.
7. Crabtree JH, Burchette RJ, Siddiqi RA, Huen IT, Hadnott LL, Fishman A, et al. The efficacy of silver-ion implanted catheters in reducing peritoneal dialysis-related infections. Perit Dial Int 2003;23:368-74.
8. Dakal TC, Kumar A, Majumdar RS, Yadav V. Mechanistic basis of antimicrobial actions of silver nanoparticles. Front Microbiol 2016;7:1831.
9. Singh A. Hydroxyapatite, a biomaterial: Its chemical synthesis, characterization and study of biocompatibility prepared from shell of garden snail, Helix aspersa. Bull Mater Sci 2012;35:1031-8.
10. Revathi M, Letticia M, Malathy D. Microwavsynthesis of silver nanoparticles by polyol method and testing their synergistics antibacterial activity in the presence of vancomycin. Asian J Pharm Clin Res 2018;11:288-93.
11. Russell SW, Luptak KA, Carlos TA, Alford TL, Pizziconi V. Chemical and structural evolution of sol-gel-derived hydroxyapatite thin films under rapid thermal processing. J Am Ceram Soc 1996;79:837-43.
12. Ciobanu CS, Massuyeau F, Constantin LV, Predoi D. Structural and physical properties of antibacterial ag-doped nano-hydroxyapatite synthesized at 100 C. Nanoscale Res Lett 2011;6:613.
13. Doat A, Pelle F, Gardant N, Lebugle A. Synthesis of luminescent bioapatite nanoparticles for utilization as a biological probe. J Solid State Chem 2004;177:1179-87.
14. Rasool I, Singh A. In vitro studies of biomate ial device hydroxyapatite prepared from different routes for biomedical applications. Asian J Pharm Clin Res 2018;11:493-7.
15. Jung WK, Koo HC, Kim KW, Shin S, Kim SH, Park YH, et al. Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Appl Environ Microbiol 2008;74:2171-8.
181 Views | 108 Downloads
How to Cite
HIBU WAHID, and ANJUVAN SINGH. “STUDY OF ANTIMICROBIAL ACTIVITY OF SILVER-DOPED HYDROXYAPATITE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 8, July 2019, pp. 243-5, doi:10.22159/ajpcr.2019.v12i18.34603.
Original Article(s)