TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.

  • ANNAMALAI MADURAM Department of Pharmacology, Shri Sathya Sai Medical College and Research Institute, Sri Balaji Vidyapeeth – Deemed to be University, Kancheepuram, Tamil Nadu, India.
  • RAJU KAMARAJ Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.

Abstract

Objectives: The objectives of the study were to study the antibacterial activity for the various extracts of Clausena dentata against human pathogens. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of microbiological properties is very scarce.


Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and antibacterial activity against human pathogenic bacteria such as Escherichia coli, Salmonella Typhi, Klebsiella pneumonia, Vibrio cholerae, and Staphylococcus aureus and compared with ciprofloxacin.


Results: Qualitative chemical tests revealed the presence of various phytochemicals such as alkaloids, glycosides, carbohydrate, proteins and amino acids, phytosterols, and volatile oil. The antibacterial activity result reveals that all the extracts were are more active against V. cholerae. The activity against Pseudomonas aeruginosa was mild.


Conclusion: The activity against V. cholerae was comparable with that of 5 μg/mL ciprofloxacin at the concentration of C. dentata 40 μg/mL. The orders of antibacterial activity against human pathogenic bacteria are hexane, methanol, and chloroform extract of C. dentata.

Keywords: Clausena dentata, Antibacterial activity, In vitro, Disc diffusion, Extracts

Author Biographies

ANNAMALAI MADURAM, Department of Pharmacology, Shri Sathya Sai Medical College and Research Institute, Sri Balaji Vidyapeeth – Deemed to be University, Kancheepuram, Tamil Nadu, India.

Associate professor in pharmacology, Department of pharmacology, Sri sathya sai Medical college &Reseach Centre, kancheepuram (Dt), Tamil nadu, india

RAJU KAMARAJ, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.

Professor ,

Department of Pharmacognosy,

SRM College of Pharmacy,

SRM Institute of Science & Technology

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ANNAMALAI MADURAM, and RAJU KAMARAJ. “TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Nov. 2019, pp. 69-73, doi:10.22159/ajpcr.2020.v13i1.35553.
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