POROSITY EXAMINATION OF DEMINERALIZED DENTIN MATERIAL MEMBRANE AS GUIDED BONE REGENERATION
Objective: The human skeleton is one of the body parts, which is able to repair itself from any damage. Bone damages could be caused by trauma, tumors, infections, congenital defects, and also due to surgical procedures. If the bone is not treated well, alveolar bone would lose its volume by 40‑60% within 3 years. Guided bone regeneration (GBR) is a technique using a barrier membrane that is placed into the bone defect to provide bone cells with space intended for bone regeneration to increase bone formation which grows slower than fibrous tissue. The pore size of the barrier membrane is very important to prevent excessive fibrous tissue into the defects site and to facilitate neovascularization and the bone formation. The aims of this study are to determine the porosity of demineralized dentin material membrane (DDMM).
Method: Bovine dentin was conducted through demineralization and freeze-drying. Dentine was cut into a size of 5×5 mm with a thickness of 0.3 mm. Double packaging and sterilization were undertaken using gamma irradiation rays. Membrane porosity was examined through scanning electron microscopy (SEM) examination. SEM micrographs were converted into binary images and further analyzed by ImageJ software.
Results: Microscopic observations and quantitative measurements in this study show that the average pore size of the DDMM sample was 3.4082±0.052 μm with a ratio of 3.3562 μm−3:4602 μm, while the pore size of the Jason membrane sample was 3.9175±0.052 μm with a ratio of 3.8655 μm−3:9695 μm.
Conclusions: The average microporosity size of the samples seen is 3–5 μm. This porosity size is ideal for GBR membranes that could increase cell proliferation and aggregation to defect sites, as well as good permeability, neovascularization, and nutrient transport.
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