STUDY OF ANTIBACTERIAL AND ANTIFUNGAL EFFECT OF THE ESSENTIAL OILS OF CHENOPODIUM AMBROSIOIDES L. AND ROSMARINUS OFFICINALIS L.

  • MAMMAD ZINEB Department of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, University Ibn Tofaïl, BP 133, 14000 Kénitra, Morocco.
  • TORMAL DJASSINRA Department of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, University Ibn Tofaïl, BP 133, 14000 Kénitra, Morocco.
  • KRIBII ABDERAHIM Department of Chemistry, Laboratory of Separation Processes, Team of Environment and Applied Chemistry, Faculty of Sciences, University Ibn Tofaïl, BP 133, 14000, Kenitra, Morocco.
  • OUNINE KHADIJA Department of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, University Ibn Tofaïl, BP 133, 14000 Kénitra, Morocco.

Abstract

Objective: This work looks at the valuation of two medicinal plants growing wild in the region of Gharb (Chenopodium ambrosioides L. and Rosmarinus officinalis L.) through the study of their antibacterial and antifungal effects vis-à-vis Xanthomonas fragariae, Pseudomonas aeruginosa, Staphylococcus aureus, and two fungal strains; Fusarium solani and Botrytis cinerea.


Methods: The extraction of essential oils from each plant was carried out by hydrodistillation in a Clevenger-type apparatus. The antibacterial and antifungal tests of essential oils are carried out according to the method of diffusion using sterile discs (or aromatograms). Results are expressed in inhibition areas.


Results: Antibacterial activity of essential oil of R. officinalis L. is best compared to that of C. ambrosioides L. with an area of inhibition that is shared by 1661.06 and 1384.74, respectively, for bacteria X. fragariae and P. aeruginosa, while this area is between 2122.64 and 2826 mm2 for F. solani and B. cinerea. Moreover, the essential oils of these two plants by the method of disk diffusion feature a very significant inhibitory effect on X. fragariae and P. aeruginosa, whereas they have no effect on S. aureus.


Conclusion: The essential oils of C. ambrosioides L. and R. officinalis L. could be used as preservatives in food, to protect them from microbial or fungal spoilage.

Keywords: Rosmarinus officinalis L., Chenopodiumam brosioides L., Antimicrobial activity, Antifungal, Essential oil

References

1. Salhi S, Fadli M, Zidane L, Douira A. Floristic and ethnobotanical study of medicinal plants in city of Kénitra (Morocco). Lazaroa 2010;31:133-46.
2. Tabuti JR, Lye KA, Dhillion SS. Traditional herbal drugs of Bulamogi, Uganda: Plants, use and administration. J Ethnopharmacol 2003;88:19-44.
3. Romero CD, Chopin SF, Buck G, Martinez E, Garcia M, Bixby L. Antibacterial properties of common herbal remedies of the southwest. J Ethnopharmacol 2005;99:253-7.
4. Nedialkov PT, Nikolov SD, Kokanova-Nedialkova Z. The genus Chenopodium: Phytochemistry, ethnopharmacology and pharmacology. Pharmacogn Rev 2009;3:280-306.
5. Ba?er KH, Demirci B, Demirci F, Koçak S, Akinci C, Malyer H, et al. Composition and antimicrobial activity of the essential oil of Achillea multifida. Planta Med 2002;68:941-3.
6. Dorman HJ, Deans SG. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. J Appl Microbiol 2000;88:308-16.
7. Cabanis Y, Chabouis L, Chabouis F. Végéteaux et Groupements Végéteaux de Madagascar et des Madagaskareignes. Vol. 4. Tananarive: BDPA; 1969.
8. Bricha S. Study of the Behaviour of Pseudomonas aeruginosa with Regard to Environmental Factors and Decontamination Procedures Applied in the Food Industry. Thesis-faculty of Science, Kénitra, Morocco; 2011. p. 71-152.
9. Djassinra T, Khouidi S, Oulkheir S, Ounine K. Detection de Xanthomonas fragariae au éme niveau des serres de la région du Gharb, proceeding du 8émecongrès de l’association marocaine de protection des plantes-AMPP. November. Rabat; 2012. p. 139-153.
10. Ouraïni D, Agoumi A, Ismaïli-Alaoui M, Alaoui K, Cherrah Y, Amrani M, et al. Étude de l’activité des huiles essentielles de plantes aromatiques à propriétés antifongiques sur les différentes étapes du développement des dermatophytes. J Phytothérapie 2005;4:147-57.
11. Ali-Emmanuel N, Moudachirou M, Akakpo AJ, Quetin-Leclercq J. Activités antibactériennes in vitro de Cassia alata, Lantana camara et Mitracarpus scaber sur Dermatophilus congolensis isolé au Bénin. Revue Élev Méd Vét Pays Trop 2002;55:183-7.
12. Iqbal A, Farrukh A, Mohammad O. Modern Phytomedicine. Weinheim: Wiley-VCH Verlag GmbH, Co., KGaA; 2006. pp 384.
13. Clevenger JF. Apparatus for the determination of volatile oil. J Am Pharm Assoc 1928;17:346-51.
14. Remmal A, T-Elaraki A, Bouchikhi T, Rhayour K, Ettayibi M. Improved method for determination of antimicrobial activity of essential oils in agar medium. J Essent Oil Res 1993;5:179-84.
15. Satrani B. Ghanmi M, farah A, aafi A, fougrach H, bourkhiss B. Composition chimique et activité antimicrobienne de l’huile essentielle de Cladanthus mixtus. Bull Soc Pharm Bordeaux 2008;146:85-96.
16. Atikbekkara F, Bousmaha L, Talebbendiab SA, Boti JB, Casanova J. Chemical composition of the essential oil of Rosmarinus officinalis L growing spontaneously and cultivated from the Tlemcen. J Biol Health 2007;7:1.
17. Bozin B, Mimica-Dukic N, Samojlik I, Jovin E. Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. J Agric Food Chem 2007;55:7879-85.
18. Aghfir M, Kouhila M, Jamali A, Mohamed A. Convective Solar Drying for the Conservation of Resmary Leaves (Rosmarinus officinalis). 13th International Days of Thermal Drying. Albi, France; 2007. p. 28-30.
19. Benjilali B, Zrira S. Aromatic and Medicinal Plants. Strengths of the Sector and Requirements for Sustainable Development. Morocco: Hassan II Agricultural and Veterinary Institute Rabat Morocco. 2005.
20. Silou T, Taty-Loumbou F, Chalchat JC. Study of the effect of solar drying on the yield and chemical composition of essential oils extracted from the leaves of Eucalyptus citriodora. Ann Fals Exp Chim 2002;960:287-301.
21. Bourkhiss M, Hnach M, Bourkhiss B, Ouhssine M, Chaouch A, Satrani B. Drying effect on the content and chemical composition of essential oils of Tetraclinis articulata (Vahl) masters. Agrosolutions 2009;20:44-8.
22. Inouye S, Abe S. New approach to anti-infective aromatherapy. Phytotherapy 2007;1:2-4.
23. Kishore N, Chansouria JP, Dubey NK. Antidermatophytic action of the essential oil of Chenopodium ambrosioides and an ointment prepared from it. India Phytother Res 1999;10:453-5.
24. Jardim CM, Jham GN, Dhingra OD, Freire MM. Composition and antifungal activity of the essential oil of the Brazilian Chenopodium ambrosioides L. J Chem Ecol 2008;34:1213-8.
25. Teixeira B, Marques A, Ramos C, Serrano C, Matos O, Neng NR, et al. Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. J Sci Food Agric 2013;93:2707-14.
26. Lambert PA. Cellular impermeability and uptake of biocides and antibiotics in gram-positive bacteria and mycobacteria. J Appl Microbiol 2002;92 Suppl:46S-54S.
27. Chao SC, Young DG, Oberg GJ. Screening for inhibitory actvity of essential oils on selected bacteria, fungi and viruses. J Essent Oil Res 2000;12:639-49.
28. Robin F, Aggoune-Khinache N, Delmas J, Naim M, Bonnet R. Novel VIM metallo-beta-lactamase variant from clinical isolates of Enterobacteriaceae from Algeria. Antimicrob Agents Chemother 2010;54:466-70.
Statistics
182 Views | 106 Downloads
Citatons
How to Cite
MAMMAD ZINEB, TORMAL DJASSINRA, KRIBII ABDERAHIM, and OUNINE KHADIJA. “STUDY OF ANTIBACTERIAL AND ANTIFUNGAL EFFECT OF THE ESSENTIAL OILS OF CHENOPODIUM AMBROSIOIDES L. AND ROSMARINUS OFFICINALIS L.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Nov. 2019, pp. 81-84, doi:10.22159/ajpcr.2020.v13i1.35875.
Section
Original Article(s)