ENIGMATIC INDUCTION OF CYTOMIXIS IN ALLIUM CEPA ROOT MERISTEM BY AGLAIA EDULIS ROXB. LEAF EXTRACT AND ITS PHYTOCHEMICAL RATIONALE
Objective: The present study aims to analyze the potential of Aglaia edulis Roxb. leaf extract to induce cytological aberrations in Allium cepa root meristem and to determine the phytoconstituents in the extract.
Methods: Cytotoxicity evaluation of the leaf methanolic extract was done using Allium cepa assay using various concentrations. Volatile phytoconstituents in the extract were determined using gas chromatography–mass spectrometry analysis.
Results: Considerable number of cytomictic cells along with other aberrations was observed. The occurrence of cytomixis was found to be dose dependent where it ranged from 6.58±0.35 to 29.45±0.45. The percentage of cytomictic cells among the total aberrant cells was observed between 35.19±1.67 and 77.39±1.39. The phytochemical analysis of the plant extract revealed the presence of active secondary metabolites.
Conclusion: The synergistic action of the active compounds might have triggered the phenomenon of cytomixis which, in turn, could be exploited for the production of polyploids.
2. Lattoo SK, Khan S, Bamotra S, Dhar AK. Cytomixis impairs meiosis and influences reproductive success in Chlorophytum comosum (Thunb) Jacq.-an additional strategy and possible implications. J Biosci 2006;31:629-37.
3. Singhal VK, Kaur D, Kumar P. Effect of cytomixis on the pollen size in ‘Sea buckthorn’ (Hippophae rhamnoides L., Elaeagnaceae). Cytologia 2008;73:167-72.
4. Pierre PM, de Souza SM. Cytomixis in plants: Causes, mechanisms and consequences. Braz J Biol Sci 2011;9:231-40.
5. Guan JZ, Wang JJ, Cheng ZH, Liu Y, Li ZY. Cytomixis and meiotic abnormalities during microsporogenesis are responsible for male sterility and chromosome variations in Houttuynia cordata. Genet Mol Res 2012;11:121-30.
6. Mursalimov SR, Deineko EV. How cytomixis can form unreduced gametes in tobacco. Plant Syst Evol 2015;301:1293-7.
7. De Nettancourt D, Grant WF. La cytogénétique de lotus (leguminosae). Cytologia 1964;29:191-5.
8. Gottschalk W. Chromosome and nucleus migration during microsporogenesis of Pisum sativum. Nucleus 1970;13:1-9.
9. De M. Cytomixis in pollen mother cells of an apomictic ornamental Ervatamia divaricata (Linn.) Alston. Cytologia 1983;48:201-7.
10. Li X, Song Z, Feng D, Wang H. Cytomixis in Thinopyrum intermedium, Thinopyrum ponticum and its hybrids with wheat. Cereal Res Commun 2009;37:353-61.
11. Tarkowska J. Cytomiksja w skórce ?usek cebuli iw skórce li?ci oraz w merystemie wierzcho?kowym korzenia Allium cepa L. Cytomixis in the epidermis of scales and leaves and in the meristems of root apex of Allium cepa L. Acta Soc Bot Pol 1960;29:149-68.
12. Cooper DC. The transfer of desoxyribose nucleic acid from the tapetum to the microsporocytes at the onset of meiosis. Am Nat 1952;86:219-29.
13. Gates RR. Pollen formation in Oenothera gigas. Ann Bot 1911;25:909-40.
14. Arnoldy W. Contributionsto the morphology of gymnosperms. What are the “germinal vesicle” or “Hofmeister Korperchen” in the egg of Abietineae. Flora 1900;87:194-204.
15. Kornicke M. Uber Ortsveranderung von Zellkarnern. New Delhi: SB Niederrhein, Ges, Natureur-und and Heilkunde Bon; 1901. p. 14-25.
16. Kumar G. Induced cytomixis in chickpea (Cicer arietinum L.). Nucleus 2002;45:24-6.
17. Kostritsyna TV, Soldatov IV. Cytomixis in apical meristems of Prunus domestica L.×Persica vulgaris mill. Hybrid’ shoots. Genetika 1991;27:1790-4.
18. Klyuchareva MV. Extrusion of nuclear material in proembryos of gramineous plants (Morphology). USA: Doklady Botanical Sciences-Akademiia Nauk; 1983.
19. Wang XY, Yu CH, Li X, Wang CY, Zheng GC. Ultrastructural aspects and possible origin of cytoplasmic channels providing intercellular connection in vegetative tissues of anthers. Russ J Plant Physiol 2004;51:97-106.
20. Sidorchuk YV, Deineko EV, Shumny VK. Peculiarities of cytomixis in pollen mother cells of transgenic tobacco plants (Nicotiana tabacum L.) with mutant phenotype. Cell Tissue Biol 2007;1:570-6.
21. Bobak M. Cytomixis as a manifestation of pathological changes after the application of trifluraline. Nucleus 1978;21:22-6.
22. Dwivedi NK, Sikdar AK, Dandin SB, Sastry CR, Jolly MS. Induced tetraploidy in mulberry I. Cytologia 1986;51:393-401.
23. Chazotte B. In: Yuste R, editor. Imaging: A Laboratory Manual. Cold Spring Harbor, New York, USA: CSHL Press; 2008.
24. Sn PM, Thoppil JE. Evaluation of cytotoxicity and apoptotic potential of Ophiorrhiza pectinata Arn.-a potent anticancer agent. Asian J Pharm Clin Res 2019;12:97-102.
25. Bhat TA, Parveen S, Khan AH. MMS-induced cytomixis in pollen mother cells of broad bean (Vicia faba L.). Turk J Bot 2006;30:273-9.
26. Kumar G, Iadav RS. Induction of cytomixis affects microsporogenesis in Sesamum indicum L. (Pedaliaceae). Ontogenez 2012;43:261-7.
27. Haroun SA. Cytomixis in pollen mother cells of Polygonum tomentosum schrank. Cytologia 1995;60:257-60.
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