ENIGMATIC INDUCTION OF CYTOMIXIS IN ALLIUM CEPA ROOT MERISTEM BY AGLAIA EDULIS ROXB. LEAF EXTRACT AND ITS PHYTOCHEMICAL RATIONALE

  • ARCHANA ELAMKULAM RAVINDRAN Department of Botany, University of Calicut, Malappuram, Kerala, India.
  • JOHN ERNEST THOPPIL Department of Botany, University of Calicut, Malappuram, Kerala, India.

Abstract

Objective: The present study aims to analyze the potential of Aglaia edulis Roxb. leaf extract to induce cytological aberrations in Allium cepa root meristem and to determine the phytoconstituents in the extract.


Methods: Cytotoxicity evaluation of the leaf methanolic extract was done using Allium cepa assay using various concentrations. Volatile phytoconstituents in the extract were determined using gas chromatography–mass spectrometry analysis.


Results: Considerable number of cytomictic cells along with other aberrations was observed. The occurrence of cytomixis was found to be dose dependent where it ranged from 6.58±0.35 to 29.45±0.45. The percentage of cytomictic cells among the total aberrant cells was observed between 35.19±1.67 and 77.39±1.39. The phytochemical analysis of the plant extract revealed the presence of active secondary metabolites.


Conclusion: The synergistic action of the active compounds might have triggered the phenomenon of cytomixis which, in turn, could be exploited for the production of polyploids.

Keywords: Aglaia edulis, cytomixis, cytological aberrations, secondary metabolites, GC-MS, polyploidy

Author Biography

JOHN ERNEST THOPPIL, Department of Botany, University of Calicut, Malappuram, Kerala, India.

Professor

Cell & Molecular Biology division

Department of Botany

University of Calicut

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RAVINDRAN, A. E., and J. E. THOPPIL. “ENIGMATIC INDUCTION OF CYTOMIXIS IN ALLIUM CEPA ROOT MERISTEM BY AGLAIA EDULIS ROXB. LEAF EXTRACT AND ITS PHYTOCHEMICAL RATIONALE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 3, Jan. 2020, pp. 168-71, doi:10.22159/ajpcr.2020.v13i3.36429.
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