EVALUATION OF THE ANTICANCER PROPERTIES OF TURMESAC® IN HUMAN CERVICAL ADENOCARCINOMA
Objective: In the current study, an extract of turmeric rhizome (Turmesac®) was evaluated for possible anticancer activity in human cervical adenocarcinoma (HeLa) cells.
Methods: Turmesac®’s ability to elicit cytotoxicity in cancer cells was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (MTT) assay, where the IC50 value was determined. Apoptosis induction and cell cycle arrest were analyzed by flow cytometry post-Turmesac® IC50 value treatment for 24 h.
Results: The determined IC50 value of Turmesac® in HeLa cells was 115.12 μg/ml. This concentration was able to induce apoptosis 2 times greater than the apoptotic standard, camptothecin, treated cells. Cell cycle arrest was observed at the G0/G1 and S phases in Turmesac® treated HeLa cells.
Conclusion: Turmesac® shows the potential of being a promising anticancer agent that may be incorporated into chemotherapies, but further study is required to elucidate the exact mechanisms involved with longer treatment duration, as would be the case in clinical trial phases.
2. Pan MH, Wu JC, Ho CT, Badmaev V. Effects of water extract of Curcuma longa (L.) roots on immunity and telomerase function. J Complement Integr Med 2017;14:20150107.
3. Soleimani V, Sahebkar A, Hosseinzadeh H. Turmeric (Curcuma longa) and its major constituent (curcumin) as nontoxic and safe substances: Review. Phyther Res 2018;32:985-95.
4. Allegra A, Innao V, Russo S, Gerace D, Alonci A, Musolino C. Anticancer activity of curcumin and its analogues: Preclinical and clinical studies. Cancer Invest 2017;35:1-22.
5. Gupta SC, Patchva S, Koh W, Aggarwal BB. Discovery of curcumin, a component of golden spice, and its miraculous biological activities. Clin Exp Pharmacol Physiol 2012;39:283-99.
6. Sa G, Das T. Anti cancer effects of curcumin: Cycle of life and death. Cell Div 2008;3:1-14.
7. Bhat SV, Amin T, Nazir S. Biological activities of Turmeric (Curcuma longa Linn.)-an overview. BMR Microbiol 2015;1:1-5.
8. Hamidpour R, Hamidpour S, Hamidpour M, Sohraby M, Hamidpour R. Turmeric (Curcuma longa): From a variety of traditional medicinal applications to its novel roles as active antioxidant, anti-inflammatory, anti-cancer and anti-diabetes. Int J Pharmacol Phytochem Ethnomed 2015;1:37-45.
9. Bhaumik S, Anjum R, Rangaraj N, Pardhasaradhi BV, Khar A. Curcumin mediated apoptosis in AK-5 tumor cells involves the production of reactive oxygen intermediates. FEBS Lett 1999;456:311-4.
10. Chandrasekaran C, Sundarajan K, Edwin J, Gururaja G, Mundkinajeddu D, Agarwal A. Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction. Pharmacognosy Res 2013;5:71-9.
11. Shehzad A, Lee YS. Molecular mechanisms of curcumin action: Signal transduction. Biofactors 2013;39:27-36.
12. Gupta AP, Khan S, Manzoor MM, Yadav AK, Sharma G, Anand R, et al. Anticancer Curcumin: Natural Analogues and Structure-Activity Relationship. 1st ed. Amsterdam: Elsevier BV; 2017.
13. Karunagaran D, Rashmi R, Kumar T. Induction of apoptosis by curcumin and its implications for cancer therapy. Curr Cancer Drug Targets 2005;5:117-29.
14. Velusami CC, Boddapati SR, Srinivasa SH, Richard EJ, Joseph JA, Balasubramanian M, et al. Safety evaluation of turmeric polysaccharide extract: Assessment of mutagenicity and acute oral toxicity. Biomed Res Int 2013;2013:158348.
15. Sadashiva CT, Hussain FH, Nanjundaiah S. Evaluation of hepatoprotective, antioxidant and cytotoxic properties of aqueous extract of turmeric rhizome (Turmesac®). J Med Plants Res 2019;13:423-30.
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