METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE AND ZIDOVUDINE IN TABLET BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

  • BHOOMI D PATEL Department of Quality Assurance, Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar, Gujarat, India.
  • MEHTA BHAVYA Department of Quality Assurance, Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar, Gujarat, India.
  • ANKIT B CHAUDHARY Department of Quality Assurance, Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar, Gujarat, India.

Abstract

Objective: The objective of the study was to develop and validate reverse-phase high-performance liquid chromatography (RP-HPLC) method and apply method to tablet dosage form.


Methods: A simple, rapid, economical, precise, and accurate RP-HPLC method for simultaneous estimation of lamivudine and zidovudine in their combined dosage form has been developed.


Results: A RP-HPLC method was developed for the simultaneous estimation of lamivudine and zidovudine. In their combined dosage form has been developed. The separation was achieved by LC-C18 column (150 mm ×4.6 mm, 5 μm) and water: methanol (65:35v/v) as mobile phase, at a flow rate of 0.8 ml/min. Detection was carried out at 272 nm. Retention time of lamivudine and zidovudine was found to be 3.007 min and 4.647, respectively. The method has been validated for linearity, accuracy, and precision. The assay method was found to be linear from 50% to 150% for lamivudine and zidovudine.


Conclusion: Developed method was found to be accurate, precise, and rapid for simultaneous estimation of lamivudine and zidovudine in their combined dosage form.

Keywords: Infrared spectroscopy method, Lamivudine and zidovudine, Reverse-phase high-performance liquid chromatography method, Validation

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D PATEL, B., M. BHAVYA, and A. B CHAUDHARY. “METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE AND ZIDOVUDINE IN TABLET BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 6, Apr. 2020, pp. 73-77, doi:10.22159/ajpcr.2020.v13i6.37288.
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