BACTERIAL DNA EXTRACTION METHOD FOR THE DETECTION OF HELICOBACTER PYLORI FROM HUMAN FECAL SAMPLES

  • JHOAN GUAMÁN Department of Research, Center for Biotechnological Research and Development, Faculty of Agricultural Sciences Natural and Environmental Resources, Bolivar State University, Guaranda, Ecuador.
  • FAVIAN BAYAS-MOREJÓN Department of Research, Center for Biotechnological Research and Development, Faculty of Agricultural Sciences Natural and Environmental Resources, Bolivar State University, Guaranda, Ecuador.
  • ELENA BRITO Clinical Laboratory Degree, Faculty of Health Sciences, National University of Chimborazo, Riobamba, Riobamba, Ecuador.
  • NORMA PAREDES Nursing Degree, Faculty of Health and Human Sciences, Bolivar State University, CP: 020150, Guaranda-Ecuador.

Abstract

Objective: The work proposed the implementation of a method of DNA extraction for the detection of the pathogen from 50 stool samples.


Methods: A method of DNA extraction with Chelex resin was applied and then detected by conventional polymerase chain reaction (PCR).


Results: By PCR, 11 samples were positive for Helicobacter pylori.


Conclusions: The Chelex extraction methodology allows obtaining DNA with quality necessary to be detected by PCR, making it a fast methodology for its diagnostic application.

Keywords: DNA extraction, Helicobacter pylori, Polymerase chain reaction, Gastritis

References

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GUAMÁN, J., F. BAYAS-MOREJÓN, E. BRITO, and N. PAREDES. “BACTERIAL DNA EXTRACTION METHOD FOR THE DETECTION OF HELICOBACTER PYLORI FROM HUMAN FECAL SAMPLES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 6, Apr. 2020, pp. 38-40, doi:10.22159/ajpcr.2020.v13i6.37344.
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