PROTECTIVE EFFECT OF AQUEOUS EXTRACT OF LEAVES OF MURRAYA KOENIGII, AGAINST ALUMINUM CHLORIDE-INDUCED OXIDATIVE STRESS IN RAT LIVER AND KIDNEY
Objective: The aim of the current study was to investigate the hepatoprotective, nephroprotective, and cholesterol-lowering activity of aqueous extract of Murraya koenigii (MK) leaves against AlCl3-induced oxidative stress in rats.
Methods: Wistar albino rats were distributed into six groups (6 each). Group I (control), and Group II administered with distilled water, and aluminum chloride (AlCl3), (40 mg/kg body weight [b.w], oral), respectively. Group III rats were treated with standard Vitamin E (100 mg/kg b.w, p.o) and AlCl3 (40 mg/kg b.w, oral). Group IV, V, and VI received aqueous extract of leaves of Murraya koenigii (AEMK) (100 mg/kg b.w, peroral [p.o], 200 mg/kg b.w, p.o, and 400 mg/kg b.w, p.o), respectively, for a period of 35 days.
Results: Histopathological examination was observed deformities in hepatic and renal tissues due to aluminum exposure which augment the aforementioned results. Coadministration of AEMK along with Al significantly restored the serum biomarkers to their near-normal levels and has the ability to overcome Al-induced oxidative stress, manifested by a significant reduction in hepatic and renal malondialdehyde level. It increased cellular antioxidant defense, particularly by increasing GPx, glutathione, GR, and catalase levels, preserved normal hepatic and renal histological architecture.
Conclusion: It could be concluded that AEMK has significant radical scavenging activity and can mop up Al-induced toxicity, suggesting hepatoprotective and nephroprotective potential.
2. Al-Kahtani AM, Abdel-Moneim MA, El-Sayed, MW. The influence of taurine pretreatment on aluminum chloride induced nephrotoxicity in Swiss albino mice. Histopathology 2014;29:45-55.
3. Farina M, Rotta LN, Soares FA. Jardim AF, Souza DO, Rocha JB. Hematological changes in rats chronically exposed to oral aluminum. Toxicology 2005;209:29-37.
4. Stohs SJ, Bagchi D, Hassoun E, Bagchi M. Oxidative mechanisms in the toxicity of chromium and cadmium ions. J Environ Pathol Toxicol Oncol 2000;20:77-88.
5. Mailloux RJ, Lemire J, Appama VD. Hepatic response to aluminum toxicity: Dyslipidemia and liver disease. Exp Cell Res 2011;317:2231-8.
6. Nan Z, Ping L, Lina H. Protection of aluminum chelating agent against cardiac toxicity in rats exposed to aluminum chloride. J Shandong Univ 2009;4:125-7.
7. Al-Hashem F. Camel’s milk protects against aluminum chloride-induced toxicity in the liver and kidney of white albino rats. Am J Biochem Biotechnol 2009;5:98-108.
8. Nehru B, Anand P. Oxidative damage following chronic aluminium exposure in adult and pup rat brains. J Trace Elem Med Biol 2005;19:203-8.
9. Schulze-Bergkamen H, Schuchmann M, Fleischer B, Galle PR. The role of apoptosis versus oncotic necrosis in liver injury: Facts or faith? J Hepatol 2006;44:984.
10. Lippi U, Guidi G. A new colorimetric ultramicromethod for serum glutamic-oxalacetic and glutamic-pyruvic transaminase determination. Clin Chim Acta 1970;28:431-7.
11. Belfeld A, Goldberg D. Revised assay for serum phenyl phosphatase activity using 4- amino-antipyrine. Enzyme 1971;12:561-73.
12. Schmidt M, Eisenburg J. Serum bilirubin determination in newborn infants. A new micromethod for the determination of serum of plasma bilirubin in newborn infants. Fortschr Med 1975;93:1461-6.
13. Patton CJ, Crouch SR. Determination of urea (urease-modified Berthelot reaction). Anal Chem 1977;49:464-9.
14. Bonsens KE, Taussky S. Determination of serum creatinine. J Chem 1984;27:648-60.
15. Richmond W. Cholesterol enzymatic colorimetric test chop PAP method of estimation of total cholesterol in serum. Clin Chem 1973;191:1350.
16. Fossati P, Prencipe L. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem 1982;28:2077-80.
17. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;17:214-26.
18. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
19. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science 1973;179:588-90.
20. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978;52:302-10.
21. Newairy AS, Salama AF, Hussien HM, Yousef MI. Propolis alleviates aluminum-induced lipid peroxidation and biochemical parameters in male rats. Food Chem Toxicol 2009;47:1093-8.
22. Mangood SA, Kamal AM, Haggag AM. Propolis protection from toxicity caused by aluminum chloride in male rats. Appl Radiat Isot 2012;44:623-33.
23. Beidokhti MN, Prakash HS. Antioxidant and anti-inflammatory potential of selected medicinal plants of Lamiaceae family. Int J Pharm Pharm Sci 2013;5 Suppl 1:100-4.
24. Iswariya S, Uma TS. Evaluation of in vitro anti-inflammatory and antimicrobial activity of aqueous and methanolic seed extracts of Citrullus lanatus. Int J Pharm Pharm Sci 2017;9:29-33.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.