ANTIBACTERIAL ACTIVITY FOR BIOSURFACTANT PRODUCED BY STREPTOMYCES SPP. ISOLATED FROM SOIL SAMPLES

  • SAMER M. AL-HULU Department of Environment, Environmental Sciences College, Al-Qasim Green University, Iraq.

Abstract

Objective: The goal of the present study on isolation Streptomyces from soil samples with biosurfactant activity and antibacterial activity analysis.


Methods: (25) The soil samples were taken form Hilla city. (10) It is Streptomyces spp. They were identified. (4) Streptomyces spp. having the ability to hemolysis on blood agar that has the capacity to generate biosurfactants. Streptomyces spp. 8 displayed a high degree of activity by having a blood agar inhibition zone (25 mm).


Results: Antibacterial activity was evaluated for Streptomyces spp. 8 (Staphylococcus aureus, Escherichia Coli, and Pseudomonas aeruginosa). Streptomyces spp. 8 has greatest inhibition zone against S. aureus equal to 14 mm compared 12 mm against E. coli and 8 mm for P. aeruginosa. This Streptomyces spp. 8 characteristic was tested, it showed Gram positive with aerial mycelium gray in color on yeast malt extract agar. Negative for melanin produced on tyrosine broth medium, negative for H2S output, and pigment development, it has for the use of mannitol sucrose, glucose, and fructose as carbon source and negative for xylose.


Conclusion: Isolated Streptomyces spp. having the potential to generate biosurfactants with antibacterial activity.

Keywords: Streptomyces spp., Biosurfactant, Antibacterial activity

References

1. Aarthi M, Kamalanathan D, Balakrishnan V. Isolation of actinomycetes from the sediments of Pichavaram mangrove for set, South India and analysis their antibacterial efficay. Asian J Pharm Clin Res 2020;13:120-5.
2. Lo CW, Lai NS, Cheah HY, Wong NK, Ho CC. Actinomycetes isolated from soil samples from the Crocker Range Sabah. ASEAN Rev Biodivers Environ Conserv 2002;2002:1-7.
3. Lucas X, Senger C, Erxleben A, Gruning BA, Doring K, Mosch J, et al. StreptomeDB: a resource for natural compounds isolated from Streptomyces species. Nucleic Acids Res 2013;41:D1130-6.
4. Shubha M, Ushashi B, Veena S, Rao KV. Antimicrobial activity of Streptomyces variabilis strian-VITUMVB03 Isolated from Kanyakumari marine sediments. Asian J Pharm Clin Res 2017;10:112-6.
5. Deepika TL, Prasad AS, Kannabiran K. Production of biosurfactant and heavy metal resistance activity of Streptomyces Sp. VITDDK3-a novel halo tolerant actinomycetes isolated from saltpan soil. Adv Biol Res 2010;4:108-15.
6. Hamzah A, Sabturani N, Radiman S. Screening and optimization of biosurfactant production by the hydrocarbon-degrading Bacteria. Sains Malaysiana 2013;42:615-23.
7. Pacwa-Plociniczak M, Plaza GA, Piotrowska-Seget Z, Cameotra SS. Environmental applications of biosurfactants: Advances. Int J Mol Sci 2011;12:633-54.
8. Jacobucci DF, Oriani MR, Durrant LR. Reducing COD level on oily effluent by utilizing biosurfactant-producing bacteria. Braz Arch Biol Technol 2009;52:1037-42.
9. Kumar M, Leon V, Materano AD, Ilzins OA. Enhancement of oil degradation by co-culture of hydrocarbon degrading and biosurfactant producing Bacteria. Pol J Microbiol 2006;55:139-46.
10. Vasudevan N, Rajaram P. Bioremediation of oil sludge contaminated soil. Environ Int 2001;26:409-11.
11. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313-40.
12. Holt JG. Bergey’s Manual of Determinative Bacteriology, Actinomycetales. 9th ed. United States: Lippincott Williams & Wilkins; 2000.
13. Carillo P, Mardarz C, Pitta-Alvarez S. Isolation and selection of biosurfactant producing Bacteria. World J Microbiol Biotechnol 1996;12:82-4.
14. Cappucino JG, Sherman N, Microbiology, A Laboratory Manual. Boston, MA: Pearson Benjamin Cummings; 1999.
15. Sastry M, Ahmad A, Khan MI, Kumar R. Biosynthesis of metal nanoparticles using fungi and actinomycetes. Curr Sci 2003;85:162-70.
16. Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM, McInerney MJ. Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods 2004;56:339-47.
17. Bodour AA, Miller-Maier RM. Biosurfactants: Types, screening methods and applications. In: Encyclopedia of Environmental Microbiology. New York: Wiley; 2002. p. 750-70.
18. Richter M, Willey JM, Submuth R, Jung G, Fiedler HP. Streptofactin, a novel biosurfactant with aerial mycelium inducing activity from Streptomyces tendae Tu 901/8c. FEMS Microbiol Lett 1998;163:165-71.
19. Donio MB, Ronica FA, Viji VT, Velmurugan S, Jenifer JS, Michaelbabu M, et al. Halomonas sp. BS4, A biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance. Springerplus 2013;2:149.
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AL-HULU, S. M. “ANTIBACTERIAL ACTIVITY FOR BIOSURFACTANT PRODUCED BY STREPTOMYCES SPP. ISOLATED FROM SOIL SAMPLES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 7, Apr. 2020, pp. 109-10, doi:10.22159/ajpcr.2020.v13i7.37472.
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