Isolation, screening of rhizosphere fungi antagonistic to rice stem rot disease pathogen Sclerotium oryzae catt.,

  • Nagam Nenkateswarlu sri venkateswara university Tirupati A.P, India.
  • Ongolu Sireesha
  • Sani Aishwayra
  • Tartte Vijaya
  • Aruga Sriramulu

Abstract

Objective: The production of antifungal substances by rhizosphere fungi has long been recognized and this knowledge is entering practical life
through the use of fungal antagonists to protect crops against their fungal pathogens.
Methods: In this study rhizosphere fungi isolation in serial dilution plate technique, and their antimicrobial properties against plant pathogen tested
by dual culture, disc diffusion method. Effect of these fungi in seed germination and growth promoting activity was measured in pot culture method.
Results and conclusion: Rhizosphere fungi it has become clear that in addition to diffusible substances evidence has accumulated that these bioactive
compounds are not only able to promote seed germination and plant growth, but also to strongly inhibit pathogenic fungal growth. As the demand
for organic products and the need to render agriculture more sustainable are rising, finding new environmentally friendly crop protection strategies
is essential. In this perspective, the newly discovered capacity of fungal bioactive compounds to efficiently repel phytopathogenic fungi in laboratory
experiments holds great promise.
Keywords: Rhizosphere, Biological control, Sclerotium oryzae, Seed germination.

Author Biography

Nagam Nenkateswarlu, sri venkateswara university Tirupati A.P, India.
SVU

References

Reference

1. Yang, L.Z.; Zhou, L.; Xu, H.; Qin, B.F. Isolation and identification and antifungal activity of a Penicillium. Acta Agric. Boreali-Occid. Sin. 2009, 18, 98–102.
2. Sireesha.O, Venkateswarlu. N, 2013, In vitro evaluation of Botanicals and panchagavya against leaf blast fungus Pyricularia grisea., Asian J Pharm Clin Res, Vol 6 Suppl 5, 2013, 84-86.
3. Goldman, L.R. Encyclopedia of Public Health: Fungicides. Cited on 6 June 2008.
4. ILO. 1996. Wage workers in agriculture: condition of employment and work; sectoral activities programme. Report for discussion at the tripartite meeting on improving conditions of employment and work of agricultural wage workers in the context of economic restructuring. International Labor Organization, Geneva.
5. Miller, G.T. 2004. Sustaining the Earth, 6th editon. Thompson Learning, Inc. Pacific Grove California, Chapter 9, p. 211-216.
6. Whipps, J.M. Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 2001, 52, 487–511.
7. Yang, J.H., Liu, H.X., Zhu, G.M., Pan, Y.L., Xu, L.P. and Guo, J.H. (2008) Diversity analysis of antagonists from rice-associated bacteria and their application in biocontrol of rice diseases.J Appl Microbiol 104,91–104.
8. Wiwattanapatapee, R., Chumthong, A., Pengnoo, A. and Kanjanamaneesathian, M. (2007) Effervescent fast-disintegrating bacterial formulation for biological control of rice sheath blight.J Control Release 119, 229–235.
9. Chumthong, A., Kanjanamaneesathian, M., Pengnoo, A. and Wiwattanapatapee, R. (2008) Water-soluble granules containing Bacillus megaterium for biological control of rice sheath blight: formulation, bacterial viability and efficacy testing.World J Microb Biot24, 2499–2507.
10. Pal, K.K. and B. M. Gardener. 2006. Biological control of plant pathogens. The Plant Health Instructor DOI: 10.1094/PHI-A-206-117-02. APSnet. 25 p
11. Leelasuphakul, W., Sivanunsakul, P. and Phongpaichit, S. (2006) Purification, characterization and synergistic activity of beta-1, 3-glucanase and antibiotic extract from an antagonistic Bacillus subtilis NSRS 89–24 against rice blast and sheath blight. Enzyme MicrobTechnol38, 990–997.
12. Rangaswamy G and Mahadevan A (1999). Diseases of crop plants in India. (4th edition) Prentice Hall of India Pvt. Ltd. New Delhi, pp. 607.
13. Barnett,H.L. and Hunter, B.B. (1972). Illustrated genera of imperfect fungi . 3rd edition, Burgess Publishing Co., 273 pp.
14. Johnson, Leander F., and Elroy A. Curl. 1972. Methods for Research on the Ecology of Soil-Borne Plant Pathogens. 426 So. Sixth St., Minneapolis, MN 55415: Burgess Publishing Company.
15. Baker. E. F and R.J. Cook, 1974. Biological control of plant pathogens. W.H. Freeman & Co. Sanfransisco, 433pp.
16. Wang J, Liu H, Kurt’an T, M’andi A, S’andor A, Jia L, Zhang H, Guo Y (2011)Protolimonoids and norlimonoids from the stem bark of Toona ciliata var. pubescens. Org Biomol Chem 9(22):7685–7696
17. Anitha Chowdary, K. (1997). Studies on sclerotial wilt of bell pepper (Capsicum annum L.). Ph.D (Ag.) Thesis submitted to Acharya N.G. Ranga Agricultural University, Hyderabad.
18. Abdelmonem AM, Rasmy MR (2000). Fungi and Bacteria associated with mangrove seed and measures for their control. Arab J. Plant Prot. 18:28-34.
19. Elad, Y., I. Chet, and J. Katan. 1980. Trichoderma harzianum : A biocontrol agent affective against. Sclerotium rolfsi and Rhizoctonia solani. Phytopathology 70:19-121.
20. Patale, S.S. and D.S. Mukadam. 2011. Management of plant pathogenic fungi by using Trichoderma. Bioscience Discovery 2(1): 36-37.
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How to Cite
Nenkateswarlu, N., O. Sireesha, S. Aishwayra, T. Vijaya, and A. Sriramulu. “Isolation, Screening of Rhizosphere Fungi Antagonistic to Rice Stem Rot Disease Pathogen Sclerotium Oryzae catt.,”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 54-57, https://innovareacademics.in/journals/index.php/ajpcr/article/view/3758.
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Original Article(s)