ANTI-INFLAMMATORY PROFILING OF SEED AND RIND EXTRACTS OF AMOMUM SUBULATUM

  • SUPRIYA AGNIHOTRI Department of Pharmacognosy, Chandigarh College of Pharmacy, Landran, Mohali.

Abstract

Objective: The study aimed to evaluate the anti-inflammatory activity of Amomum subulatum (greater cardamom) seed and rind extracts in Wistar rats.


Methods: The seed and rind of A. subulatum were air-dried in the shade, powdered, and subjected to 80% hydroalcoholic extraction in the Soxhlet apparatus. The anti-inflammatory activity of the seed and rind extracts of A. subulatum was evaluated by in vivo and in vitro methods.


Results and Discussion: In vivo studies, namely, carrageenan-induced rat paw edema, cotton pellet granuloma, and formaldehyde-induced arthritis model confirmed the anti-inflammatory potential of seed and rind extracts of A. subulatum. It was found that rind extract exhibited better inhibition of inflammation as compared to seed extract. A. subulatum rind extract at the dose of 500 μg/ml exhibited best results for in vitro studies, namely, inhibition of albumin denaturation (73% inhibition), antiproteinase action (58% inhibition), membrane stabilization, heat-induced hemolysis, hypotonicity-induced hemolysis (54% inhibition), anti-lipoxygenase activity.


Conclusions: The results of the study showed that the rind extract of A. subulatum (greater cardamom) possesses significant anti-inflammatory potential in various in vivo and in vitro anti-inflammatory models in the experimental animals.

Keywords: Amomum subulatum, Carrageenan, In vitro anti-inflammatory, Anti-lipoxygenase

References

1. Anonyms. Wealth of India, a Dictionary of Indian Raw Materials and Industrial Products. 1st Supplement Series (Raw Materials). New Delhi: National Institute of Science Communication; 2004.
2. Anonymous. Quality Standards of Indian Medicinal Plants. Vol. 3. New Delhi: Indian Council of Medical Research; 2005. p. 1-8.
3. Anonymous. Indian Herbal Pharmacopoeia. Vol. 1. Mumbai: Indian Drug Manufacturers Association; 2002. p. 1-10.
4. Rao CB, Rao TN, Suryaprakasam S. Cardamonin and alpinetin from the seeds of Amomum subulatum. Planta Med 1976;29:39139-42.
5. Nadkarni AK. In Indian Materia Medica (Dr. K.M. Nadkarni’s). 3rd ed. Mumbai, Maharashtra: Popular Prakashan Pvt. Ltd.; 1982. p. 40-3.
6. Jafri M, Farah A, Javed K, Singh S. Evaluation of the gastric antiulcerogenic effect of large cardamom (fruits of Amomum subulatum Roxb). J Ethnopharmacol 2001;75:89-94.
7. Dhuley JN. Anti-oxidant effects of cinnamon (Cinnamomum verum) bark and greater cardamom (Amomum subulatum) seeds in rats fed a high-fat diet. Indian J Exp Biol 1999;37:238-42.
8. Kikuzaki H, Kawai Y, Nakatani NJ. 1,1-Diphenyl-2-picrylhydrazyl radical-scavenging active compounds from greater cardamom (Amomum subulatum Roxb.), Nutr Sci Vitaminol 2001;47:167-71.
9. Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962;111:544-7.
10. Winter CA, Porter CS. Effect of alterations in side-chain upon anti-inflammatory and liver glycogen activities of hydrocortisone esters. J Am Pharm Sci 1957;46:515-9.
11. Brownlee G. Effect of deoxycortone and ascorbic acid on formaldehyde induced arthritis in normal and adrenalectomized rats. Lancet 1950;1:157-9.
12. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-73.
13. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharmacol Sci 2010;2:146-55.
14. Oyedepo OO, Femurewa AJ. Anti-protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Int J Pharmacongn 1995;33:65-9.
15. Sadique J, Al-Rqobahs WA, Bughaith, EIGindi Ar. The bioactivity of certain medicinal plants on the stabilization of RBS membrane system. Fitoterapia 1989;60:525-32.
16. Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Dikshit VJ, Saraf MN. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) Loud. wood oil. Indian J Exp Biol 1999;37:258-61.
17. Azeem AK, Dilip C, Prasanth SS, Junise V, Shahima H. Anti-inflammatory activity of the glandular extracts of Thunnus alalunga. Asia Pac J Med 2010;3:412-20.
18. Das SN Chatterjee S. Long term toxicity study of ART-400. Indian Indg Med 1995;16:117-23.
19. Gandhidasan R, Thamaraichelvan A, Baburaj S. Anti inflammatory action of Lannea coromandelica by HRBC membrane stabilization. Fitoterapia 1991;62:81-3.
20. Shenoy S, Shwetha K, Prabhu K, Maradi R, Bairy KL, Shanbhag T. Evaluation of anti-inflammatory activity of Tephrosia purpurea in rats. Asian Pac J Trop Med 2010;3:193-5.
21. Vadivu R, Lakshmi KS. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchinensis (Lour) Moore ssp Laurina. Bangladesh J Pharmacol 2008;3:121-4.
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AGNIHOTRI, S. “ANTI-INFLAMMATORY PROFILING OF SEED AND RIND EXTRACTS OF AMOMUM SUBULATUM”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 6, May 2020, pp. 204-8, doi:10.22159/ajpcr.2020.v13i6.37625.
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