FORMULATION AND EVALUATION OF CAFFEINE-LOADED SOLID LIPID NANOPARTICLES TO TREAT CLINICAL MASTITIS
Objective: The objective of the present study was to formulate and evaluate caffeine-loaded solid lipid nanoparticles (SLNs) in the treatment of clinical mastitis.
Methods: These were prepared by homogenization technique using stearic acid, Tween 80, and chloroform as excipients. Pre-formulation studies such as UV spectrophotometry, Fourier transform infrared (FTIR), and differential scanning calorimetry (DSC) were performed for the drug. Entrapment efficiency and in vitro dissolution studies were carried out for prepared SLNs and the optimum formulation (F2) was taken for further studies such as FTIR, DSC, SEM, particle size, and zeta potential analysis.
Results: Obtained results stated that prepared SLNs are roughly spherical in nature and are in nano range. These were incorporated in Carbopol gel and further evaluation studies such as pH, spreadability, viscosity, homogenicity, and in vitro drug diffusion studies were carried out. All the results stated that prepared nanogel has shown sustained release of drug. Antimicrobial study was carried out using Staphylococcus aureus and it was confirmed by the appearance of zone of inhibition.
Conclusion: Nanogel that contains caffeine SLNs with 1:2 ratio drug:lipid has shown good in vitro release. Sustained release of caffeine drug till 12 h was achieved by delivering it in the form of nanogel.
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