PHYTOCHEMICAL AND PHARMACOLOGICAL SCREENING OF MALLOTUS PHILIPPENSIS AGAINST CCL4- AND ATT-INDUCED HEPATOTOXICITY IN RATS
Keywords:Mallotus philippensis, CCl4, ATT, Biochemical parameters and histopathological studies
Objective: Mallotus philippensis (Mp) is locally known as kamala and is a large woody multipurpose medicinal tree belonging to the family of Euphorbiaceae. Mp possess a wide variety of activities such as skin problem, bronchitis, antifungal, worm infestation (tapeworm) eye disease, cancer, diabetes, and diarrhea. Hence, the present study was intended to evaluate methanolic fruits extract of Mp for hepatoprotective activities.
Methods: The hepatoprotective activity was studied by CCl4 at the dose of 1 ml/kg of body weight in liquid olive oil in the ratio of 1:1 and ATT (isoniazid − 7.5 mg/kg, rifampicin − 10 mg/kg, and pyrazinamide − 35 mg/kg b.w.) induced models. Acute toxicity study and preliminary phytochemical screening were also studied to evaluate the toxicity.
Results: No toxicity profile was observed in rats after oral administration of the methanolic fruits extract at the dose of 2 g/kg body weight. The different dose of 300 mg/kg and 500 mg/kg administered with the extract of Mp. There was a significant (p<0.001) reduction in biochemical parameters with respect to control. Phytochemical screening of the fruits extract revealed the presence of tannins, alkaloids, flavonoids and saponins, and terpenoids.
Conclusion: It can be concluded that the hepatoprotective activity elucidated by Mallotus philippensis could be mainly due to the presences of high-value class of compound like the phenolic group as the major content in the plant.
Sandhu DS, Heinrich M. The use of health foods, spices and other botanicals in the Sikh community in London. Phytother Res 2005;19:633-42.
Gupta MP, Solís PN, Calderón AI, Guionneau-Sinclair F, Correa M, Galdames C, et al. Medical ethnobotany of the Teribes of Bocas del Toro, Panama. J Ethnopharmacol 2005;96:389-401.
Sgro C, Clinard F, Ouazir K, Chanay H, Allard C, Guilleminet C, et al. Incidence of drug-induced hepatic injuries: A French population-based study. Hepatology 2002;36:451.
Tschrich: As an Handbuckder Pharmakognosie Leipzig, Vol. 3; 1923.
Kokate CK. Practical Pharmacognosy, Preliminary Phytochemical Screening. 1st ed. New Delhi: Vallabh Prakashan; 1986. p. 111.
Darbar S, Bose A, Chatterjee N, Roy B, Chattaraj TK, Pal TK.Antioxidant and hepatoprotective activity of ascorbic acid against diclofenac induced hepatotoxicity in rats. Indian Drugs 2009;46:35-41.
Molander DW, Sheppard E, Payne MA. Serum transaminase in liver disease. J Am Med Assoc 1957;163:1461-5.
Ramakrishna S, Geetha KM, Gopal B, Kumar PR, Madav PC, Umachandar L. Effect of Mallotus Philippensis Muell. Arg leaves against hepatotoxicity of carbon tetrachloride in rats. Int J Pharm Sci Res 2011;2:74-83.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.