ACUTE AND SUBACUTE TOXICITY STUDY OF THE ETHANOL EXTRACTS OF PUNICA GRANATUM (LINN). WHOLE FRUIT AND SEEDS AND SYNTHETIC ELLAGIC ACID IN SWISS ALBINO MICE

Authors

  • B. Satheesh Kumar Bhandary
  • K.P Sharmila
  • N. Suchetha Kumari
  • S. Vadisha Bhat

Keywords:

Punica granatum (L), Ellagic acid, ethanolic extract, acute toxicity, subacute toxicity

Abstract

Objective: The objective of the present study was to evaluate the acute and sub-acute toxicity profile of ethanolic extracts of Punica granatum (L).whole fruit (EPWF) and seeds (EPS) and synthetic ellagic acid (EA) in Swiss albino mice following OECD guidelines.

 Methods: In acute toxicity study, mice were orally administered 2000 mg/kg body weight of extracts and synthetic compound. In subacute toxicity study, the animals were orally administered with the extracts and synthetic compound (2000 mg/kg body weight) daily for 28 days whereas, control group received normal saline.

Results: There were no behavioral alterations or mortality recorded in the treated groups. The LD50 value was more than 2000 mg/kg body weight. Test groups did not record any significant alterations (p>0.05) in body weight gain, food and water intake. The hematological and biochemical parameters and organ weights did not record any significant alterations (p>0.05) in the treated groups when compared to control. A detailed examination of histoarchitecture of the liver and kidney did not reveal any observable cellular damage in the treated groups compared to control.

Conclusion: The overall finding of this study suggests that Punica granatum (L) whole fruit (EPWF) and seeds (EPS) ethanolic extract and synthetic ellagic acid (EA) is safe up to 2000 mg/kg body weight oral administration and can be considered as non toxic.

Keywords: Punica granatum (L), Ellagic acid, ethanolic extract, acute toxicity, subacute toxicity

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Published

2013-09-01

How to Cite

Kumar Bhandary , B. S., K. Sharmila, N. Suchetha Kumari, and S. Vadisha Bhat. “ACUTE AND SUBACUTE TOXICITY STUDY OF THE ETHANOL EXTRACTS OF PUNICA GRANATUM (LINN). WHOLE FRUIT AND SEEDS AND SYNTHETIC ELLAGIC ACID IN SWISS ALBINO MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 8, Sept. 2013, pp. 192-8, https://innovareacademics.in/journals/index.php/ajpcr/article/view/385.

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