EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF VERNONIA AMYGDALINA LEAF EXTRACTS AS AN AUXILIARY IN NATURAL HAIR SHAMPOO
Objective: This study evaluated the phytochemical compounds, antioxidant, and antimicrobial activities of Vernonia amygdalina (VA) leaf extract and used it as an auxiliary herbal constituent in natural hair shampoo.
Methods: VA leaf was macerated in various solvents. All crude extracts were phytochemical compounds screened and determined the antioxidant and antimicrobial activities before formulating a natural shampoo formula. Natural shampoos were evaluated with physic-chemical properties and sensory satisfaction.
Results: About 95% ethanolic extract was the most suitable substrate for product development. It reveals the inhibition zone from 7.00±0.00 to 15.00±1.00 mm and possesses a broad spectrum of antibacterial agents against both Gram-positive and Gram-negative bacteria. The MICs and minimum bactericidal concentrations value ranged from 6.250 to 100 mg/ml and 25 to >200 mg/ml, respectively. About 95% ethanolic extract also revealed high antioxidant activity. The IC50 value of DPPH of 95% ethanolic extract was 1.88±0.02 μg/ml (% inhibition of 85.73±0.01) and the ferric reducing ability power was 23.00±0.50 mg AAE/100 gDW. These high biological activities may be due to a broad range of phytochemical compounds, including saponins, tannins, flavonoids, terpenoids, steroids, cardiac glycosides, and alkaloids.
Conclusions: The VA leaf was suitable to use as an ingredient in natural shampoos with a low detrimental effect on normal skin flora. The shampoo with 0.00188% of 95% ethanolic extract was the most suitable formula in terms of characteristics and stability. This formula also obtained the highest satisfaction level under our sensory evaluation.
2. Revansiddappa M, Sharadha R, Abbulu K. Formulation and evaluation of herbal antidandruff shampoo. J Pharmacol Phytochem 2018;7:764-7.
3. Adaramoye O, Ogungbenro B, Anyaegbu O, Fafunso M. Protective effects of extracts of Vernonia amygdalina, Hibiscus sabdariffa and Vitamin C against radiation-induced liver damage in rats. J Radiat Res 2008;49:123-31.
4. Alara OR, Abdurahmana NH, Ukaegbub CI, Kabbashi NA. Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques. J Taibah Univ Sci 2019;13:414-22.
5. Ilondu EM. Antifungal activities of three Vernonia spp. Phytochemical composition and efficacy of ethanolic leaf extracts of some. J Biopestic 2010;6:165-72.
6. Alara OR, Abdurahman NH, Abdul Mudalip SK, Olalere OA. Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia. J King Saud Univ Sci 2017;31:495-99.
7. Onasanwo SA, Oyebanjo OT, Ajayi AM, Olubori MA. Anti-nociceptive and anti-inflammatory potentials of Vernonia amygdalina leaf extract via reductions of leucocyte migration and lipid peroxidation. J Intercult Ethnopharmacol 2017;6:192-8.
8. Jisaka M, Ohigashi H, Takegawa K, Huffman MA, Koshimizu K. Antitumoral and antimicrobial activities of bitter sesquiterpene lactones of Vernonia amygdalina, a possible medicinal plant used by wild chimpanzees. Biosci Biotechnol Biochem 1993;57:833-4.
9. Owoeye O, Yousuf S, Akhtar MN, Qamar K, Dar A, Farombi EO, et al. Another anticancer elemanolide from Vernonia amygdalina Del. Int J Biol Chem Sci 2010;4:226-34.
10. Ngatu NR, Okajima MK, Yokogawa M, Hirota R, Takaishi M, Eitoku M, et al. Anti-allergic effects of Vernonia amygdalina leaf extracts in hapten-induced atopic dermatitis-like disease in mice. Allergol Int 2012;61:597-607.
11. Farombi EO, Owoeye O. Antioxidative and chemopreventive properties of Vernonia amygdalina and garcinia bioflavonoid. Int J Environ Res Public Health 2011;8:2533-55.
12. Luo X, Jiang Y, Fronczek FR, Lin C, Izevbigie EB, Lee S, et al. Isolation and structure determination of a sesquiterpene lactone (vernodalinol) from Vernonia amygdalina extracts. Pharm Biol 2011;49:464-70.
13. Harborne JB. Phytochemical Methods, a Guide to Modern Techniques of Plant Analysis. London: Chapman & Hall; 1998. p. 182-90.
14. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth Enzymol 1999;299:152.
15. Tinrat S, Sila-Asna M. Phytochemical screening and influence of extraction solvents on antioxidant and antimicrobial activities of Asparagus racemosus wild. Root, Int J Phytomed 2017;9:343-51.
16. Jayalakshmia B, Raveeshab KA, Muralic M, Amruthesh KN. Phytochemical, antibacterial and antioxidant studies on leaf extracts of piper betle L. Int J Pharm Pharm Sci 2015;7:23-9.
17. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 1996;239:70-6.
18. Bauer AW, Kirby WM, Sherris JC, Turk M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1996;45:493-6.
19. Kiessoun K, Roland MN, Mamounata D, Yomalan K, Sytar O, Souz A, et al. Antimicrobial profiles, antidiarrheal and antipyretic capacities of phenol acid rich-fractions from Ximenia america L., (Olacaceae) in wistar albino rats. Int J Pharm Pharm Sci 2018;10:62-8.
20. Aghel N, Moghimipour B, Dana RA. Formulation of a herbal shampoo using total saponins of Acanthophyllum squarrosum. Iran J Pharm Res 2007;6:167-72.
21. Manikar AR, Jolly CI. Evaluation of commercial herbal shampoos. Int J Cosmet Sci 2000;22:385-91.
22. Al Badi K, Khan SA. Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos. Beni Suef Univ J Basic Appl Sci 2014;3:301-5.
23. Cartagena E, Montanaro S, Bardón A. Improvement of the antibacterial activity of sesquiterpene lactones. Rev Latinoam Química 2008;36:43-51.
24. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
25. Sibanda T, Okoh AI. The challenges of overcoming antibiotic resistance: Plant extracts as potential sources of antimicrobial and resistance modifying agents. Afr J Biotechnol 2007;6:2886-96.
26. Kolawole S, Obueh H, Ukachi A. Antibacterial effect of Vernonia Amygdalina and Ocimum Gratissimum on some Bacteria that cause opportunistic infections in HIV/AIDS patients. J Nutr Health Food Sci 2018;6:1-5.
27. Evbuomwan L, Chukwuka EP, Obazenu EI, Ilevbare L. Antibacterial activity of Vernonia amygdalina leaf extracts against multidrug resistant bacterial isolates. J Appl Sci Environ Manage 2018;22:17-21.
28. Shah SR, Ukaegbu CI, Hamid HA, Alara OR. Evaluation of antioxidant and antibacterial activities of the stems of Flammulina velutipes and Hypsizygus tessellatus (white and brown var. Extracted with different solvents). J Food Meas Charact 2018;12:1947-61.
29. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interv Aging 2018;13:757-72.
30. Satyal P, Dosoky NS, Poudol A, Setzer WN. Essential oil constituents and their biologic activities from the leaves of Cassia fistula growing in Nepal. J Med Aromat Plants 2012;3:1-4.
31. Moure A, Cruz JM, Franco D, Domínguez JM, Sineiro J, Domínguez H, et al. Natural antioxidants from residual sources. Food Chem 2001;72:145-71.
32. Arekemase MO, Oyeyiola GP, Balogun KI. Assessment of bitter leaf (Vernonia amygdalina) on some selected pathogenic microorganisms from university of Ilorin teaching hospital. J Microb Biotech Food Sci 2013;2:2360-65.
33. Lyumugabe F, Uyisenga JP, Bayingana C, Songa EB. Antimicrobial activity and phytochemical analysis of Vernonia aemulans, Vernonia amygdalina, Lantana camara and Markhamia lutea leaves as natural beer preservatives. Am J Food Technol 2017;12:35-42.
34. Ogundare AO. Antibacterial properties of the leaf extracts of Vernonia amygdalina, Ocimum gratissmum, Corchorois olitorius and Manihot palmate. J Microbiol Antimicrob 2011;3:77-86.
35. Habtom S, Gebrehiwo S. In vitro antimicrobial activities of crude extracts of Vernonia amygdalina and Croton macrostachyus against some bacterial and fungal test pathogens. J Phytopharmacol 2019;8:57-62.
36. Okigbo RN, Igwe DI. Antimicrobial effects of Piper guineense Uziza and Phyllantus amarus Ebe-benizo on Candida albicans and Streptococcus faecalis. Acta Microbiol Immunol Hung 2008;54:353-66.
37. Ochei J, Kolhatkar A. Isolation and identification Bacteria. In: Medical Laboratory Science Theory and Practice. New York: McGraw-Hill Publishing Company Limited; 2008. p. 644-58.
38. Baran R, Maibah HI. Cosmetic dermatology in children. In: Text Book of Cosmetic Dermatology. London: CRC Press; 1998. p. 507-8.
39. AlQuadeib BT, Eltahir EK, Banafa RA, Al-Hadhairi LA. Pharmaceutical evaluation of different shampoo brands in local Saudi market. Saudi Pharm J 2018;26:98-106.
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