SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLE FROM COUROUPITA GUIANENSIS LEAF EXTRACT AND ITS EFFECT ON CLINICAL PATHOGENS

DEVAKUMAR JOSEPH; SINDHUJA BASKARAN; MADURI NAGARAJAN; SUDHA SIVASUBRAMNIAN

doi:10.22159/ajpcr.2020.v13i10.38859

Authors

DEVAKUMAR JOSEPH Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
SINDHUJA BASKARAN Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
MADURI NAGARAJAN Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
SUDHA SIVASUBRAMNIAN Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i10.38859

Keywords:

Silver nanoparticle, Couroupita guianensis, Antimicrobial activity, Nil, Fourier transform infrared

Abstract

Objective: In this study, a rapid and simple approach was applied for the synthesis of silver nanoparticle (AgNP) from aqueous leaf extract of Couroupita guianensis. The plant extract acts as an antimicrobial agent and is also used to synthesis AgNP.

Methods: Ultraviolet (UV)–visible spectrophotometer was used to characterize synthesized AgNP and to identify the compounds responsible for the reduction of silver ions, the functional groups present in plant extract were investigated by Fourier transform infrared. Agar well diffusion method was used for the antibacterial activity of synthesized AgNPs.

Results: UV–visible spectrophotometer showed an absorbance peak in the range of 405 nm. The AgNPs showed antibacterial activities against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, Bacillus sp, Klebsiella sp, and Pseudomonas sp.

Conclusion: Nowadays, silver-based topical dressing has been widely used as a treatment for infections such as wounds, burns, and chronic ulcer.

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Author Biography

DEVAKUMAR JOSEPH, Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.

PG and Research Department

References

Krithiga N, Rajalakshmi A, Jayachitra A. Green synthesis of silver nanoparticles using leaf extract of Clitoria ternatea and Solanum nigrum and study of its antibacterial effect against common nosocomial pathogens. J Nanosci 2015;2015:928204.

Gurunathan S, Park JH, Han JW, Kim JH. Comparative assessment of the apoptotic potential to nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cells: Targeting p53 for anticancer therapy. Int J Nanomed 2015;10:4203-22.

Li WR, Xie XB, Shi QS, Zeng HY, Ou-Yang YS, Chen YB. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microb Biotechnol 2010;8:1115-22.

Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, et al. Fungus-mediated synthesis of silver nanoparticle synthesis. Nano Lett 2001;1:515-9.

Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: Green synthesis and their antimicrobial activities. Coll Interface 2009;145:83-96.

Staquicini FI, Ozawa MG, Moya CA, Driessen WH, Barbu EM, Nishimori H, et al. Systemic combinatorial peptide selection yields a non-canonical iron-mimicry mechanism for targeting tumors in a mouse model of human glioblastoma. J Clin Investig 2011;161:1 61-73.

Lin PC, Lin S, Wang PC, Sridhar R. Techniques for physicochemical characterization of nanomaterials. Biotechnol Adv 2014;32:711-26.

Gurunathan S, Han JW, Kim ES, Prk JH, Kim JH. Reduction of grapheme oxide by resveratrol: A novel and simple biological method for the synthesis of an effective anticancer nanotherapeutic molecule. Int J Nanomed 2015;10:2951-69.

Sapsford KE, Tyner KM, Dair BJ, Deschamps JR, Medintz IL. Analyzing nanomaterial bioconjugates: A review of current and emerging purification and characterization techniques. Anal Chem 2011;83:4453-88.

AI-Dhabi NA, Balachandran C, Raj MK, Duraipandiyan V, Muthukumar C, Ignacimuthu S, et al. Antimicrobial, antimycobacterial and antibiofilm properties of Couroupita guianensis. BMC Complement Altern Med 2012;12:242.

Shekhawat MS, Anusuya P, Kannan N, Manokari M, Revathi J, Illavarasi V. Green synthesis of silver nanoparticles using Couroupita guianensis Aubl. and their characterization. Int J Green Herbal Chem 2013;2:1041-9.

Devaraj P, Kumari P, Aarti C, Renganathan A. Synthesis and characterization of silver nanoparticles using cannonball leaves and their cytotoxic activity against MCF-7 cell line. J Nanotechnol 2013;2012;598328.

Kumar TV, Murthy JS, Rao MN, Bhargava Y. Evaluation of silver nanoparticles synthetic potential of Couroupita guianensis Aubl. Flower buds extract and their synergistic antibacterial activity. 3 Biotech 2016;6:92.

Sivakumar T, Rathimeena T, Shankar T, Ponmanickam P. Production of silver nanoparticles synthesis of Couroupita guianensis plant extract against human pathogen and evaluations of antioxidant properties. Int J Life Sci 2015;3:333-40.

Sivakumar T, Shankar T, Vijayabaskar P, Geetha G. Efficacy of Couroupita guianensis against selected human pathogens. Adv Biol Res 2012;6:59-63.

Fu M, Li Q, Sun D, He Lu, Deng X, Wang H, et al. Rapid preparation process of silver nanoparticles by bioreduction and their characterizations. Chin J Chem Eng 2006;14:114-7.

Sastry M, Ahmad A, Khan MI, Kumar R. Biosynthesis of metal nanoparticles using fungi and actinomycetes. Curr Sci 2003;85:202-6.

Shankar SS, Ahmad A, Pasricha R, Sastry M. Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J Mater Chem 2003;13:1822-6.

Mulvaney P. Surface plasmon spectroscopy of nanosized metal particles. Langmuir 1996;12:788-800.

Gole A, Dash C, Ramakrishna V, Sainkar SR. Pepsin-gold colloid conjugates: Preparation, characterization and enzymatic activity.Langmuir 2001;17:1674-9.

Sivaraman SK, Elango I, Kumar S, Santhanam V. A green protocol for room temperature synthesis of AgNPs in seconds. Curr Sci 2009;97:1055-9.

Dibrov P, Gosinl JK, Hase CC. Chemiosmotic mechanism of antimicrobial activity of Ag+ in Vibrio cholera. J Antimicro Agents Chemother 2002;46:2668-70.

Jayaraman P, Sakharkar MK, Lim CS, Tang TH, Sakharkar KR.Activity and interaction of antibiotic and phytochemical combinations against Pseudomonas aeruginosa in vitro. Int J Biol Sci 2010;6:556-68.

Jeong SH, Hwang YH, Yi SC. Antimicrobial properties of padded PP/ PE nonwovents incorporating nano-sized silver colloids. J Mater Sci 2005;40:5413-8.

Logeswari P, Silambarasan S, Abraham J. Synthesis of AgNPs using plants extract and analysis of their antimicrobial property. J Saudi Chem Soc 2012;48:007.