ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM LEAVES OF PISTACIA INTEGERRIMA (ANACARDIACEAE) STEW EX BRAND
Purpose: The ethanol extract of leaves of Pistacia integerrima (EEPI) was evaluated for antibacterial activity.
Methods: The leaves of P. integerrima were collected from wild source and authenticated by Dr. Manoj Joshi, botanist environmental education expert, Una, HP, India. Antimicrobial activity was performed using the Mueller–Hinton (MH) agar using disk diffusion method. In the MH agar media 38 g after dissolving in 1000 ml of distilled water (pH 7.3±0.2), the extract was incorporated such that concentration per ml will be 66.67 μg, 133.33 and, 166.66.
Results: The minimum inhibitory concentration recorded was 133.33 for Pseudomonas aeruginosa, Salmonella Typhi, and Klebsiella pneumonia, whereas 166.6 for Staphylococcus aureus, Coagulase-negative Staphylococcus, and Escherichia coli. The maximum zone of inhibition was found 19 mm and 18 mm for S. aureus and E. coli, respectively. S. aureus have been reported for skin pneumonia, heart valve, and bone infections whereas E. coli causes diarrhea and many other infections in children.
Conclusion: After reporting the better zone of inhibition for these two bacteria, EEPI can be used to formulate better herbal remedy against them.
2. Lavanya J, Periyar SS, Jeevitha PM, Preethi J, Aradana M. Antioxidant and antimicrobial activity of selected medicinal plants against human oral pathogens. Int J Pharm Pharm Sci 2016;8:71-8.
3. Doppalapudi S. Evaluation of antibacterial activity of seed extracts of Vigna unguiculata. Int J Pharm Pharm Sci 2014;6:75-7.
4. Vashist H, Sharma D. Ethnomedicinal survey of plant of Gharsi Village Hills and its allied area of district Solan. Int J Pharm Pharm Sci 2013;5:848-50.
5. Vashist H, Devi R, Agarwal S, Sharma D, Priyanka K, Chandran KM, et al. Anthology of inherent potentials about Pistacia integerrima. Innov Int J Med Pharm Sci 2017;2:1-2.
6. Vashist H, Jindal A, Gupta A, Jalhan S. Physicochemical and phytochemical evaluation of Pistacia integerrima stew ex brand. J Glob Pharm Technol 2012;7:24-7.
7. Vashist H, Jindal A. Pharmacognostical evaluation of Pistacia integerrima stew ex Brand. Int J Recent Adv Pharm Res 2012;2:70-7.
8. Srivastava R, Verma A, Mukerjee A, Soni N. Phytochemical, pharmacological and pharmacognostical profile of Vernonia anthelmintica: An overview. Res Rev J Pharmacogn Phytochem 2014;2:23-8.
9. Ahmada S, Alia M, Ansaria SH, Ahmed F. Phytoconstituents from the galls of Pistacia integerrima Stewart. J Saudi Chem Soc 2010;14:409-12.
10. Ramachandra YL, Ravi SB, Sujan G, Sundar RS. In-vitro antimicrobial activity of Pistacia integerrima leaf gall extract. Pharmacophore 2010;1:149-54.
11. Ghias U, Abdur R. In vitro antimicrobial profile of Pistacia integerrima galls Stewart. Middle East J Med Plants Res 2012;1:36-40.
12. Steven YC, Joshua SD, Emily E, Thomas LH, Vance GF. Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 2015;28:603-61.
13. Makvana S, Krilov LR. Escherichia coli infections. Pediatr Rev 2015;36:167-71.
14. Prasad PL, Gupta A, Mukhija G. Study of urinary tract infection in febrile children below 2 years of age. Int J Res Med Sci 2018;6:3657-62.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.