Objective: Antifungal activity of total ethanolic extract and fractions of Usnea varieties were proved against Aspergillus niger, Penicillium digitatum, and Rhizopus nigricans fungi. Methods: To perform the study of relative antifungal activity (AFR), flu

  • DAYANNA CABRA GACHA Universidad Distrital Francisco José de Caldas. Facultad de Ciencias y Educación, Bogotá, Colombia. https://orcid.org/0000-0002-9915-5816
  • LUIS POMBO OSPINA Fundación Universitaria Juan N. Corpas, Centro de investigación (GIFVTA) Bogotá, Colombia.
  • JANETH ARIAS PALACIOS Department of Microbiology, Industrial Microbiology Program, Industrial Environmental and Biotechnology Research Group, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • OSCAR RODRÍGUEZ AGUIRRE Environmental Engineering Program, Faculty of Engineering, Research Group CHOC-IZONE, Universidad El Bosque, Bogotá, Colombia.


Objective: Antifungal activity of total ethanolic extract and fractions of Usnea varieties were proved against Aspergillus niger, Penicillium digitatum, and Rhizopus nigricans fungi.

Methods: To perform the study of relative antifungal activity (AFR), fluconazole and ketoconazole were used as standards. The inhibition coefficient 50 (IC50) for each extract and the fractions was determined.

Results: It was established that the highest activity was presented by the acetone fraction with a value of 58.53. In the study of antifungal activity, the fraction that presented the best activity against P. digitatum was ethanolic with a value of 44.33, while for R. nigricans was petrol extract, with a value of 75.35 and finally for A. niger was the total extract with a value of 35.48.

Conclusions: Comparing the values obtained from the extract and the different fractions resulted in the dichloromethane fraction showing the best values.

Keywords: Aspergillus niger, Penicillium digitatum, Rhizopus nigricans, Relative antifungal activity AFR, Usnea varieties


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How to Cite
GACHA, D. C., L. P. OSPINA, J. A. PALACIOS, and O. R. AGUIRRE. “Objective: Antifungal Activity of Total Ethanolic Extract and Fractions of Usnea Varieties Were Proved Against Aspergillus Niger, Penicillium Digitatum, and Rhizopus Nigricans Fungi. Methods: To Perform the Study of Relative Antifungal Activity (AFR), Flu”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 10, Aug. 2020, pp. 171-4, doi:10.22159/ajpcr.2020.v13i10.38962.
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