• LIPI NOGAI Department of Pharmacy, Invertis Institute of Pharmacy, Invertis University, Bareilly, Uttar Pradesh, India.
  • TIRATH KUMAR Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India.
  • PANKAJ LOHUMI Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India.


Objective: The work is aimed to evaluate the anti-inflammatory and antioxidant activity of the ethanolic leaf extract of Salvia lanata.

Methods: Anti-inflammatory activity of the leaf extract of S. lanata at a dose of 100 mg/kg and 200 mg/kg against the standard drug indomethacin at a dose of 10 mg/kg i.p. was evaluated by carrageenan-induced rat paw edema and protein denaturation method. Antioxidant activity was determined by 1, 1 diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method, reducing power method, and nitric oxide scavenging assay.

Results: S. lanata leaf extract showed highly significant dose-dependent efficacy against carrageenan-induced paw edema at a dose of 200 mg/kg and lesser effect at 100 mg/kg. It inhibited heat-induced albumin denaturation with a maximum inhibition of 79.26% at 160 μg/ml. DPPH free radical scavenging activity of extract exhibited inhibition of 25.96%–87.74% within the concentration range of 10 μg/ml–160 μg/ml, nitric oxide assay from 12.26% to 79.22% in the same concentration range. In reducing power assay with an increase in concentrations, an increase in the absorbance of the reaction mixture was observed. Antioxidant activity was compared to standard drug ascorbic acid.

Conclusion: The leaf extract of S. lanata has potent anti-inflammatory and antioxidant activity.

Keywords: Salvia lanata, acute oral toxicity, anti-inflammatory, antioxidant, indomethacin, ascorbic acid

Author Biography

LIPI NOGAI, Department of Pharmacy, Invertis Institute of Pharmacy, Invertis University, Bareilly, Uttar Pradesh, India.

Department of Pharmaceutical Sciences, Kumaun university, Bhimtal campus, Nainital M.Pharm student


1. Fatima ZE, Abderrahmane BA, Seddik KH, Lekhmici AR. Antioxidant activity assessment of Tamus communis L. roots. Int J Pharm Pharm Sci 2016;8:64-71.
2. Liu J, Wang C, Wang Z, Zhang C, Lu S, Liu J. The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides. Food Chem 2011;126:261-9.
3. Namita P, Mukesh R, Tirath K. Evaluation of anti-inflammatory potential of Kigelia pinnata leaf extract in Wistar rats. Asian J Pharm Clin Res 2012;5:95-7.
4. Ekor M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 2014;4:177.
5. Murugan R, Parimelazhagan T. Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn. An in vitro approach. J King Saud Univ Sci 2014;26:267-75.
6. Medzhitov R. Origin and physiological roles of inflammation. Nature 2008;454:428-35.
7. Malaviya AN, Kapoor SK, Singh RR, Kumar A, Pande I. Prevalence of rheumatoid arthritis in the adult Indian population. Rheumatol Int 1993;13:131-4.
8. Akira S, Uematsu S, Takeuchi O. Pathogen recognition and innate immunity. Cell 2006;124:783-801.
9. Busse WW. Leukotrienes and inflammation. Am J Respir Crit Care Med 1998;157:S210-3.
10. Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: Importance of NOD2 and NALP3 in interleukin-1? generation. Clin Exp Immunol 2007;147:227-35.
11. Siracusa MC, Kim BS, Spergel JM, Artis D. Basophils and allergic inflammation. J Allergy Clin Immunol 2013;132:789-801.
12. Checker R, Sandur SK, Sharma D, Patwardhan RS, Jayakumar S, Kohli V, et al. Potent anti-inflammatory activity of ursolic acid, a triterpenoid antioxidant, is mediated through suppression of NF-?B, AP-1 and NF-AT. PLoS One 2012;7:e31318.
13. Aruoma OI. Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc 1998;75:199-212.
14. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118.
15. Lü JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: Experimental approaches and model systems. J Cell Mol Med 2010;14:840-60.
16. Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993;49:481-93.
17. Irda F, Evelyne N, Komar RW. In vitro antioxidant activities, total flavonoid, phenolic and carotenoid content from various extracts of four species Asteraceae herb. Int J Pharm Pharm Sci 2017;7:192-7.
18. Sarac N. Antioxidant, mutagenic, and antimutagenic activities of Tragopogon longirostris var. Longirostis, an edible wild plant in Turkey. Indian J Pharmacol 2015;47:414.
19. Njomen GB, Kamgang R, Oyono JL, Njikam N. Antioxidant potential of the methanol-methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress. Indian J Pharmacol 2008;40:266.
20. Li S, Chen G, Zhang C, Wu M, Wu S, Liu Q. Research progress of natural antioxidants in foods for the treatment of diseases. Food Sci Hum Wellness 2014;3:110-6.
21. Khandelwal K. Practical Pharmacognosy. Pune: Pragati Books Pvt. Ltd.; 2008.
22. Kulkarni SK. Hand Book of Experimental Pharmacology. New Delhi: Vallabh Prakashan; 1987.
23. Ullah HA, Zaman S, Juhara F, Akter L, Tareq SM, Masum EH, et al. Evaluation of antinociceptive, in-vivo and in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Complement Altern Med 2014;14:346.
24. Gülç?n ?, Oktay M, K?reçc? E, Küfrev?o?lu Ö?. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem 2003;83:371-82.
25. Ferreira IC, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from Northeast Portugal: Individual cap and stipe activity. Food Chem 2007;100:1511-6.
26. Kumaran A. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem 2006;97:109-14.
27. Mukherjee KS, Bhattacharya MK, Ghosh PK. A triterpene acid constituent of Salvia lanata. Phytochemistry 1982;21:2416-7.
28. Mukherjee KS, Ghosh PK, Mukherjee RK. Diterpenoid quinones of Salvia lanata. Phytochemistry 1983;22:1296-7.
29. Mukherjee KS, Ghosh PK, Badruddoza S. Diterpenoid quinones of Salvia lanata. Phytochemistry 1981;20:1441.
30. Shishodia S, Majumdar S, Banerjee S, Aggarwal BB. Ursolic acid inhibits nuclear factor-?B activation induced by carcinogenic agents through suppression of I?B? kinase and p65 phosphorylation: Correlation with down-regulation of cyclooxygenase 2, matrix metalloproteinase 9, and cyclin D1. Cancer Res 2003;63:4375-83.
31. Sarkar MA, Biswas PR, Samanta AM. In vivo anti-inflammatory and in vitro antioxidant studies on methanolic and aqueous extract of Leucas indica Linn. Asian J Pharm Clin Res 2013;6:284-90.
32. Shukla P, Shukla P, Mishra SB, Gopalakrishna B. Screening of anti-inflammatory and antipyretic activity of Vitex leucoxylon Linn. Indian J Pharmacol 2010;42:409.
33. Mansouri MT, Hemmati AA, Naghizadeh B, Mard SA, Rezaie A, Ghorbanzadeh B. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw oedema in rats. Indian J Pharmacol 2015;47:292.
24 Views | 48 Downloads
How to Cite
NOGAI, L., T. KUMAR, and P. LOHUMI. “EVALUATION OF SALVIA LANATA LEAF EXTRACT FOR ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 12, Dec. 2020, pp. 113-7, doi:10.22159/ajpcr.2020.v13i12.39516.
Original Article(s)