SANATIVE EFFECT OF MULTIHERBAL FORMULATION – AKSS16-LIV01 ON CCL4-INDUCED HEPATIC DYSFUNCTION IN MICE

  • SOUMENDRA DARBAR Department of Life Science and Biotechnology, Faculty of Science, Jadavpur University, Kolkata, West Bengal, India.
  • SRIMOYEE SAHA Department of Physics, Jadavpur University, Kolkata, West Bengal, India.
  • KAUSIKISANKAR PRAMANIK Department of Chemistry, Jadavpur University, Kolkata, West Bengal, India.
  • ATISKUMAR CHATTOPADHYAY Department of Life Science and Biotechnology, Faculty of Science, Jadavpur University, Kolkata, West Bengal, India.

Abstract

Objectives: Hepatic dysfunction is a critical public health problem affecting the global population, characterized by excessive deposition of extracellular matrix components due to increased matrix production and decreased matrix degradation. The present work was aimed to evaluate hepatoprotective effect of AKSS16-LIV01 a newly developed multiherbal formulation against carbon tetrachloride (CCl4)-induced liver dysfunction in Swiss albino mice to establish it as a bench to bedside formulation catering to the various facets of hepatic malfunction.


Methods: Thirty-six Swiss adult albino Wister mice divided into six groups. Group-I control untreated animals, Group-II received AKSS16-LIV01 (400 mg/kg), Group-III received CCl4 (1 ml/kg-bw), Group-IV received AKSS16-LIV01 (200 mg/kg) after 2 weeks CCl4 induction, Group-V received AKSS16-LIV01 (400 mg/kg) after 2 weeks CCl4 induction, and Group-VI received standard drug silymarin (100 mg/kg). At the end of the experimental period, all the animals were fasted overnight and blood was collected through retro-orbital plexus for preparation of serum and was analyzed for biochemical parameters, lipid profile, and total plasma protein. Liver tissue was collected for histological study.


Results: The combined plant extract including six Indian medicinal herbs and three medicinal spices (AKSS16-LIV01) showed significant hepatoprotective effect by controlling the various essential biochemical parameters in serum. Moreover, treatment with AKSS16-LIV01 raised the level of serum total protein, normalizes the serum biochemical and lipid profiles parameters. Pre-treatment with AKSS16-LIV01 in mice also restored the alteration of various liver parameters such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, blood urea nitrogen, total bilirubin, and direct bilirubin on CCl4-induced liver damage. Gross liver morphology and normal histological examination of the liver also supported hepatic protection by AKSS16-LIV01.


Conclusion: Taken together, these results suggest that AKSS16-LIV01 may induce remarkable protective effects against hepatic injury induced by CCl4 treatment.

Keywords: Hepatoprotective, Natural therapy, Herbal formulation, Liver function test, Histopathological studies

References

1. Svegliati-Baroni G, De Minicis S, Marzioni M. Hepatic fibrogenesis in response to chronic liver injury: Novel insights on the role of cell-to-cell interaction and transition. Liver Int 2008;28:1052-64.
2. Winau F, Quack C, Darmoise A, Kaufmann SH. Starring stellate cells in liver immunology. Curr Opin Immunol 2008;20:68-74.
3. Khanjarsim V, Karimi J, Khodadadi I, Mohammadalipour A, Goodarzi MT, Solgi G, et al. Ameliorative effects of nilotinib on CCl4 induced liver fibrosis via attenuation of RAGE/HMGB1 gene expression and oxidative stress in rat. Chonnam Med J 2017;53:118-26.
4. Mohamed MK, Khalaf MM, Abo-Youssef AM, Abo-Saif AA. Caffeineas a promising antifbrotic agent against CCL4-induced liver fibrosis. Drugs 1985;85:17143.
5. Wu J, Zern MA. Hepatic stellate cells: A target for the treatment of liver fibrosis. J Gastroenterol 2000;35:665-72.
6. Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model. Crit Rev Toxicol 2003;33:105-36.
7. Pierce RA, Glaug MR, Greco RS, Mackenzie JW, Boyd CD, Deak S. Increased procollagen mRNA levels in carbon tetrachloride-induced liver fibrosis in rats. J Biol Chem 1987;262:1652-58.
8. Hernández-muñoz R, Díaz-muñoz M, Suárez J, de Sánchez VC. Adenosine partially prevents cirrhosis induced by carbon tetrachloride in rats. Hepatology 1990;12:242-8.
9. Frei B, Higdon JV. Antioxidant activity of tea polyphenols in vivo. Evidence from animal studies. J Nutr 2003;133:3275S-84S.
10. Asadi-Samani M, Bahmani M, Rafieian-Kopaei M. The chemical composition, botanical characteristic and biological activities of Borago officinalis: A review. Asian Pac J Trop Med 2014;7:S22-8.
11. Asadi-Samani M, Kooti W, Aslani E, Shirzad H. A systematic review of Iran’s medicinal plants with anticancer effects. J Evid Based Complementary Altern Med 2016;21:143-53.
12. Gholamian-Dehkordi N, Luther T, Asadi-Samani M, Mahmoudian- Sani MR. An overview on natural antioxidants for oxidative stress reduction in cancers; a systematic review. Immunopathol Persa 2017;3:e12.
13. Kooti W, Hasanzadeh-Noohi Z, Sharafi-Ahvazi N, Asadi-Samani M, Ashtary-Larky D. Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa). Chin J Nat Med 2016;14:732-45.
14. Mahmoudian-Sani MR, Asadi-Samani M, Luther T, Saeedi- Boroujeni A, Gholamian N. A new approach for treatment of Type 1 diabetes: Phytotherapy and phytopharmacology of regulatory T cells. J Renal Injury Prevent 2017;6:158-63.
15. Thompson M, Jaiswal Y, Wang I, Williams L. Hepatotoxicity: Treatment, causes and applications of medicinal plants as therapeutic agents. J Phytopharmacol 2017;6:186-93.
16. Karunamoorthi K, Jegajeevanram K, Vijayalakshmi J, Mengistie E. Traditional medicinal plants: A source of phytotherapeutic modality in resource-constrained health care settings. J Evid Based Complement Altern Med 2013;18:67-74.
17. Bello IA, Ndukwe GI, Audu OT, Habila JD. A bioactive flavonoid from Pavetta crassipes K. Schum. Org Med Chem Lett 2011;1:1-5.
18. Rabee AA, Bennasir H. Hesperidin an antioxidant flavonoid prevents carbon tetrachloride-induced hepatic toxicity in male albino rats. J Innov Pharm Biol Sci 2018;5:127-32.
19. De S, Suresh R, Babu AM, Aneela S. In-vivo hepatoprotective activity of methanolic extracts of Sphaeranthus amaranthoides and Oldenlandia umbellata. Pharmacogn J 2017;9:98-101.
20. Adhikari A, Darbar S, Chatterjee T, Das M, Polley N, Bhattacharyya M, et al. Spectroscopic studies on dual role of natural flavonoids in detoxification of lead poisoning: Bench-to-bedside preclinical trial. ACS Omega 2018;3:15975-87.
21. Kashyap M, Hynd B, Robinson K. A rapid and simple method for measurement of total protein in very low density lipoproteins by the Lowry assay. J Lipid Res 1980;21:491-5.
22. Adhikari A, Polley N, Darbar S, Pal SK. Therapeutic potential of surface functionalized Mn3O4 nanoparticles against chronic liver diseases in murine model. Mater Focus 2017;6:280-9.
23. Darbar S, Saha S, Pramanik K, Chattopadhyay A. Preliminary acute oral toxicity study of a newly developed herbal formulation. World J Pharm Res 2018;7:924-30.
24. Rajina P, Dominic S. Toxicity evaluation of ethanolic extract of Astercantha longifolia seeds. Hyg J Drugs Med 2013;5:152-63.
25. Dahiru D, Obidoa O. Pretreatment of albino rats with aqueous leaf extract of Ziziphus mauritiana protects against alcohol-induced liver damage. Trop J Pharm Res 2007;6:705-10.
26. Halliwell B, Gutteridge JM. Oxygen free radicals and iron in relation to biology and medicine: Some problems and concepts. Arch Biochem Biophys 1986;246:501-14.
27. Nordmann R, Ribière C, Rouach H. Implication of free radical mechanisms in ethanol-induced cellular injury. Free Radic Biol Med 1992;12:219-40.
28. Kanchana N, Sadiq AM. Hepatoprotective effect of Plumbago zeylanica on paracetamol induced liver toxicity in rats. Int J Pharm Pharm Sci 2011;3:151-4.
29. Singh SK, Rajasekar N, Raj NA, Paramaguru R. Hepatoprotective and antioxidant effects of Amorphophallus campanulatus against acetaminophen induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2011;3:202-5.
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DARBAR, S., S. SAHA, K. PRAMANIK, and A. CHATTOPADHYAY. “SANATIVE EFFECT OF MULTIHERBAL FORMULATION – AKSS16-LIV01 ON CCL4-INDUCED HEPATIC DYSFUNCTION IN MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 1, Jan. 2021, pp. 101-6, doi:10.22159/ajpcr.2021.v14i1.39595.
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