Antiproliferativeand Antioxidant Effects of Eruca Sativa (Jarjeer) Leaves Extract on Carcinoma of Women’s Breast

  • Manar Elkady Urology&Nephrology Center, Mansoura University,Mansoura,Egypt.

Abstract

Carcinoma of women’s breast is the second highest common cancer worldwide and is considered to be the main reason for cancer-related mortality in women. The traditional or standard chemotherapy have different severe side effects on cancer patients. However, "plant-derived” natural products have a considerable potential as substitute drugs for preventing and treating diseases. Objective:The aim of this work is to investigate the influence of Eruca sativa leaves extract on the cell viability of the breast carcinoma cell line (MCF-7).Methods: In vitro, breast cancer cell line (MCF-7) treated by Eruca sativaleaves extract for 48 hours. Thecell viability, proliferation and apoptosis were assessed using colorimetric MTT assay, flow cytometric technique, antioxidant enzymes (SOD, CAT) measurement. Results: This study demonstrated that the incubation of MGF-7 cells with E. sativa for 48 h caused a significativereduction in cell viability and proliferation of MGF-7 cell line. In parallel, E. sativa treatment induce a significant increase in apoptosis of MGF-7 cells compared to control. Moreover, flow cytometry analysis demonstrated that the inhibition of MGF-7 cell proliferation existed at the G2and M phase in the cell-division cycle. Finally, the intracellular antioxidant enzymes SOD, CAT activities were significantly increased in the administered cells compared withunadministered MCF-7 cells. Conclusions: Taken together, E. sativa treatment reduces cell viability and proliferation concomitant with enhanced antioxidant enzymes expression and apoptosis of breast cancer cell line MGF-7.This may help in protection from breast cancer or preclinical recommendation.

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Elkady, M. “Antiproliferativeand Antioxidant Effects of Eruca Sativa (Jarjeer) Leaves Extract on Carcinoma of Women’s Breast”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 2, Jan. 2021, https://innovareacademics.in/journals/index.php/ajpcr/article/view/39937.
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