ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF ERUCA SATIVA (JARJEER) LEAVES EXTRACT ON CARCINOMA OF WOMEN’S BREAST

  • SAFAA A DERBALA Urology and Nephrology Center, Mansoura University, Egypt.
  • MANAR E ELKADY Urology and Nephrology Center, Mansoura University, Egypt.
  • REHAB A ELBANHAWY Urology and Nephrology Center, Mansoura University, Egypt.
  • ABDEL-AZIZ AF Department of Biochemistry, Faculty of Science, Mansoura University, Mansoura, Egypt.

Abstract

Objective: This work aims to investigate the influence of Eruca sativa leaves extract on the cell viability of the breast carcinoma cell line (MCF-7).


Methods: In vitro, breast cancer cell line (MCF-7) treated by E. sativa leaves extract for 48 h. The cell viability, proliferation, and apoptosis were assessed using colorimetric (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric technique, and antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT]) measurement.


Results: This study demonstrated that the incubation of MGF-7 cells with E. sativa for 48 h caused a significative reduction in cell viability and proliferation of MGF-7 cell line. In parallel, E. sativa treatment induces a significant increase in apoptosis of MGF-7 cells compared to control. Moreover, flow cytometry analysis demonstrated that the inhibition of MGF-7 cell proliferation existed at the G2 and M phase in the cell-division cycle. Finally, the intracellular antioxidant enzymes SOD and CAT activities were significantly increased in the administered cells compared with unadministered MCF-7 cells.


Conclusions: Taken together, E. sativa treatment reduces cell viability and proliferation concomitant with enhanced antioxidant enzymes expression and apoptosis of breast cancer cell line MGF-7. This may help in protection from breast cancer or preclinical recommendation.

Keywords: Eruca sativa, Cell line of breast cancer, Apoptosis, DNA fragmentation, Antioxidants

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A DERBALA, S., M. E ELKADY, R. A ELBANHAWY, and A.-A. AF. “ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF ERUCA SATIVA (JARJEER) LEAVES EXTRACT ON CARCINOMA OF WOMEN’S BREAST”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 2, Jan. 2021, pp. 89-92, doi:10.22159/ajpcr.2021.v14i2.39337.
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