Antiproliferativeand Antioxidant Effects of Eruca Sativa (Jarjeer) Leaves Extract on Carcinoma of Women’s Breast
Carcinoma of women’s breast is the second highest common cancer worldwide and is considered to be the main reason for cancer-related mortality in women. The traditional or standard chemotherapy have different severe side effects on cancer patients. However, "plant-derived” natural products have a considerable potential as substitute drugs for preventing and treating diseases. Objective:The aim of this work is to investigate the influence of Eruca sativa leaves extract on the cell viability of the breast carcinoma cell line (MCF-7).Methods: In vitro, breast cancer cell line (MCF-7) treated by Eruca sativaleaves extract for 48 hours. Thecell viability, proliferation and apoptosis were assessed using colorimetric MTT assay, flow cytometric technique, antioxidant enzymes (SOD, CAT) measurement. Results: This study demonstrated that the incubation of MGF-7 cells with E. sativa for 48 h caused a significativereduction in cell viability and proliferation of MGF-7 cell line. In parallel, E. sativa treatment induce a significant increase in apoptosis of MGF-7 cells compared to control. Moreover, flow cytometry analysis demonstrated that the inhibition of MGF-7 cell proliferation existed at the G2and M phase in the cell-division cycle. Finally, the intracellular antioxidant enzymes SOD, CAT activities were significantly increased in the administered cells compared withunadministered MCF-7 cells. Conclusions: Taken together, E. sativa treatment reduces cell viability and proliferation concomitant with enhanced antioxidant enzymes expression and apoptosis of breast cancer cell line MGF-7.This may help in protection from breast cancer or preclinical recommendation.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.