• KAMNI RAJPUT Department of Botany and Microbiology, Gurukula Kangri (Deemed To Be University), Haridwar, Uttarakhand, India.
  • RAMESH CHANDRA DUBEY Department of Botany and Microbiology, Gurukula Kangri (Deemed To Be University), Haridwar, Uttarakhand, India.



Probiotics bacteria, Lactic acid bacteria, Enterococcus spp, Antidiabetic effect, Antioxidant activity, Intestinal attachment


Objective: In vitro antioxidant activity, in vivo antidiabetic property and intestinal attachment by two potential probiotic bacterial strains, namely, Enterococcus faecium and Enterococcus hirae were studied using albino rats.

Methods: Antioxidant the activity was assessed using 2,2-Diphenyl-1-picrylhydrazyl radicals scavenging assay. Alloxan was administered intraperitoneally to induce diabetic conditions in experimental rats. Animals were treated with oral administration of Enterococcus spp., such as E. faecium, and E. hirae isolated from goat and sheep milk. The control animal group received normal saline for the same days. Glibenclamide drug was used as a positive control against probiotic bacterial cells.

Results: However, administration of probiotic bacterial strains E. faecium and E. hirae, in albino rats significantly (p<0.05) at varying doses lowered blood glucose levels in diabetic rats as compared to the diabetic control group. Both the species of Enterococcus increased the bodyweight of experimental rats. However, E. faecium was the best antidiabetic strain having the antioxidant activities also in comparison to E. hirae. The attachment of probiotic bacterial cells E. faecium on the rat’s intestine wall against pathogens was examined. Furthermore, E. faecium showed its aggregation with pathogens by attachment of the intestines of albino rats. This showed that both the bacterial strains exhibited in vivo antidiabetic effect.

Conclusion: The results of this study showed that probiotic bacteria possess antioxidant, antidiabetic activities, and attachment of intestine.


Download data is not yet available.


Steven D, Ehrlich N. Solutions Acupuncture: A Private Practice Specializing in Complementary and Alternative Medicine. Diabetes VeriMed Healthcare Network; 2012. Available from: http://www.umm. edu/health/medical/altmed/condition/diabetes. [Last accessed on 2014 Apr 13].

Caballero AE, Arora S, Saouaf R, Lim SC, Smakowski P, Park JY, et al. Microvascular and macrovascular reactivity is reduced in subjects at risk for Type 2 diabetes. Diabetes 1999;48:1856-62.

Farvid MS, Siasi F, Jalai M. The impact of Vitamin C and E, magnesium and Zinc on glycemic control and insulin resistance in Type II diabetic patients. Tehran Univ Med J 2006;64:67-75.

Ceriello A. New insights on oxidative stress and diabetic complications may lead to a “causal” antioxidant therapy. Diabetes Care 2003;26:1589-96.

Vijayakumar M, Govindarajan R, Rao GM, Rao CV, Shirwaikar A, Mehrotra S, et al. Action of Hygrophila auriculata against streptozotocin-induced oxidative stress. J Ethnopharmacol 2006;104:356-61.

Prabhurajeshwar, C, Chandrakanth, RK. Development of in vitro methodologies for inhibition of pathogenic bacteria by potential probiotic Lactobacillus sps: An evidence for production of antimicrobial substances. Int J Pharm Pharm Sci 2016;8:277-86.

Gomes AC, Bueno AA, de Souza RG, Mota JF. Gut microbiota, probiotics and diabetes. Nutr J 2014;13:60.

Choudhary J, Dubey RC, Sengar G, Dheeman S. Evaluation of probiotic potential and safety assessment of Lactobacillus pentosus MMP4 isolated from mare’s lactation. Probiotics Antimicrob Proteins 2018;11:403-12.

Debapriya M, Pratima R. Evaluation of probiotic and antimicrobial properties of lactobacillus strains isolated from dairy products. Int J Pharm Pharm Sci 2016;8:230-34.

Rajput K, Dubey RC. Probiotic potential and safety characterization of Enterococcus hirae G24 isolated from indigenous raw goat milk. Int J Pharm Sci Drug Res 2020;12:1-9.

Heo SJ, Cha SH, Lee KW, Cho SM, Jeon YJ. Antioxidant activities of Chlorophyta and phaeophyta from Jeju Island. Algae 2005;20:251-60.

Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative effect of Diasulin, a polyherbal formulation in alloxan induced hyperglycemic rats. BMC Complement Altern Med 2005;5:14.

Litchfield JJ, Wilcoxon F. A simplified method of evaluating dose-effect experiments. J Pharmacol Exp Ther 1949;96:99-113.

Duzguner V, Kaya S. Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits. Free Rad Biol Med 2007;42:1481-86.

Chang S, Hypolite JA, DiSanto ME, Changolkar A, Wein AJ, Chacko S. Increased basal phosphorylation of detrusor smooth muscle myosin in alloxan-induced diabetic rabbit is mediated by upregulation of Rho-kinase β and CPI-17. Am J Physiol Renal Physiol 2006;290:650-56.

Halliwell B, Gutteridge JM. Free radicals in biology and medicine. J Free Radic Biol Med 1985;1:331-4.

Nikkilä EA, Kekki M. Plasma triglyceride transport kinetics in diabetes mellitus. Metab Clin Exp 1973;22:1-22.

Huang X, Vaag A, Hansson M, Weng J, Laurila ES, Groop L. Impaired insulin-stimulated expression of the glycogen synthase gene in skeletal muscle of Type 2 diabetic patients is acquired rather than inherited. J Clin Endocrinol Metab 2000;85:1584-90.

Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes 1991;40:405-12.

Beachey EH. Bacterial adherence: Adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J Infect Dis 1981;143:325-45.



How to Cite

RAJPUT, K., and R. CHANDRA DUBEY. “IN VITRO ANTIOXIDANT AND IN VIVO ANTIDIABETIC ACTIVITY OF TWO POTENTIAL PROBIOTIC ENTEROCOCCUS SPP. ON ALLOXAN-INDUCED DIABETIC RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 14, no. 3, Mar. 2021, pp. 94-98, doi:10.22159/ajpcr.2021.v14i3.40321.



Original Article(s)