DEVELOPMENT AND VALIDATION OF ULTRAVIOLET–VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF GLYCYRRHIZIN IN METHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA L
Objective: The pure form of glycyrrhizin was bought from Yucca Enterprises, Mumbai-37, India. The pure form of drug used in treatment of acne vulgaris disease. Pharmacognostical studies of Glycyrrhiza glabra L. indicated surprising antibacterial action against Propionibacterium acnes.
Methods: A simple, rapid, accurate, precise, and economic spectrophotometric technique for estimation of glycyrrhizin in methanolic extract of G. glabra L. have been developed. Glycyrrhizin exhibit absorbance most at 254 nm when phosphate buffer (pH-6.8) methanol is used as solvent in 70:30 proportion, so absorbance was once measured at the identical wave lengths for the determination of glycyrrhizin. Glycyrrhizin obeys the Beer Lambert’s law in the concentration range of 4–24 μg/ml.
Results: This method was validated according to International Council for Harmonization guidelines and can be adopted for the general analysis of glycyrrhizin in hydroalcoholic extract of G. glabra. The approach is simple, rapid, safe, accurate, affordable, and beneficial for standardization of the licorice products.
Conclusion: The results applied in the routine analysis and quality control of pharmaceutical dosage forms containing glycyrrhizin.
2. Xu H, Fabricant DS, Piersen CE, Bolton JL, Pezzuto JM, Fong H, et al. A preliminary RAPD-PCR analysis of Cimicifuga species and other botanicals used for women’s health. Phytomedicine 2002;9:757-62.
3. Nam C, Kim S, Sim Y, Chang I. Anti-acne effects of oriental herb extracts: A novel screening method to select anti-acne agents. Skin Pharmacol Physiol 2003;16:84-90.
4. Panichayupakaranant P, Tewtrakul S, Yuenyongsawad S. Antibacterial, anti-inflammatory and anti-allergic activities of standardised pomegranate rind extract. Food Chem 2010;123:400-3.
5. Kaur R, Kaur H, Dhindsa AS. Glycyrrhiza glabra: A phytopharmacological review. Int J Pharm Sci Res 2013;4:2470-7.
6. Ram S. A bibliometric assessment of liquorice (Glycyrrhiza glabra) research trends. Ann Lib Inf Stud 2015;62: ???.
7. Armanini D, Fiore C, Mattarello MJ, Bielenberg J, Palermo M. History of the endocrine effects of licorice. Exp Clin Endocrinol Diabetes 2002;110:257-61.
8. Baker ME. Endocrine activity of plant-derived compounds: An evolutionary perspective. Proc Soc Exp Biol Med 1995;208:131-8.
9. Norman HA, Pillai P, Baker ME. Licorice-derived compounds inhibit linoleic acid (C: 18: 2?6) desaturation in soybean chloroplasts. FEBS Lett 1995;368:135-8.
10. Der Marderosian A, Beuther JA. The Review of Natural Products. St. Louis: Facts and Comparisons; 2001. p. 405-9.
11. Tanaka A, Horiuchi M, Umano K, Shibamoto T. Antioxidant and anti?inflammatory activities of water distillate and its dichloromethane extract from licorice root (Glycyrrhiza uralensis) and chemical composition of dichloromethane extract. J Sci Food Agric 2008;88:1158-65.
12. Folkers G. In: Lund W, editor. The Pharmaceutical Codex, Principles and Practice of Pharmaceutics. London: The Pharmaceutical Press; 1994. p. 1100.
13. Nash RA, Wachter AH. Pharmaceutical Process Validation. 3rd ed., Vol. 129. New York: Revised and Expanded, Marcel Dekkar Inc.; 2003. p. 760-92.
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