ANTIFUNGAL ACTIVITY OF BACILLUS SPP. ISOLATED FROM CALOTROPIS PROCERA AIT. RHIZOSPHERE AGAINST CANDIDA ALBICANS


Mounyr Balouiri, Samira Bouhdid, El Houssaine Harki, Moulay Sadiki, Wessal Ouedrhiri

Abstract


 

Objective: This study aimed to investigate the antifungal activity of a microbial strain isolated from a non-exploited habitat (southeast region of
Morocco) against Candida albicans.
Methods: The antifungal producing strains were isolated from the rhizosphere of Calotropis procera Ait. The isolate (Cp-LMA-9) was selected based
on the primary antimicrobial screening. The optimization of the culture media for an antifungal production, and the extraction of bioactive metabolites
were performed. The bioautography and a partial characterization of these metabolites were also done.
Results: Based on the molecular identification, it was identified as Bacillus spp. with a similarity percentage of 98% with Bacillus tequilensis and
Bacillus subtilis. The maximum antifungal production against C. albicans was observed with malt extract-yeast extract-agar by solid-state fermentation
and malt extract-yeast extract-broth under stationary conditions. Antifungal fraction was extracted successfully from the solid-state fermentation by
acetone and methanol. It maintained the anti-Candida activity after heat treatment (autoclaving at 121°C for 15 minutes and boiling for 30 minutes),
within a pH range of 2-10, and after treatment with proteolytic enzymes.
Conclusion: The bacterium isolated and selected exhibit a remarkable antifungal effect against C. albicans. The resistance of the antifungal metabolites
extracted to heat, alkaline, and acidic conditions and to proteolitic enzymes making theme a promising antifungal compounds for the control of
Candida infections.

Keywords: Antifungal activity, Bacillus spp, C. albicans, Rhizosphere, Molecular identification.


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About this article

Title

ANTIFUNGAL ACTIVITY OF BACILLUS SPP. ISOLATED FROM CALOTROPIS PROCERA AIT. RHIZOSPHERE AGAINST CANDIDA ALBICANS

Date

01-03-2015

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 8 Issue 2 (March - April) 2015 Page: 213-217

Print ISSN

0974-2441

Online ISSN

2455-3891

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436 Views | 649 Downloads

Authors & Affiliations

Mounyr Balouiri
Laboratory of Medicinal and Aromatic Plants and Natural Substances, National Institute of Medicinal and Aromatic Plants – Taounate, Sidi Mohamed Ben Abdellah University, Fez, Morocco; Laboratory of Microbial Biotechnology,. Faculty of Science and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco
Morocco

Samira Bouhdid

El Houssaine Harki

Moulay Sadiki

Wessal Ouedrhiri


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