ANTI-INFLAMMATORY ACTIVITY OF TRAUMEEL (A HOMEOPATHIC PREPARATION) IN EXPERIMENTAL ANIMALS-RATS
Objective: To evaluate the anti-inflammatory effect of traumeel on acute and chronic inflammatory experimental animal models (rats).
Methods: The traumeel, a homeo medicinal preparation was tested for its efficacy in acute and chronic inflammatory rats. In acute models: Rat paw
edema method was used. In this model, rats were divided into four groups, six rats each. Group-I (NC), received 2% GA 2 ml, Group-II (PC) received
aspirin (200 mg/kg), Group-III, intravenous received low dose (6CH) and high dose (300CH) of traumeel, preparation orally. Edema was developed in
injected paw. Immediately the foot edema volume was measured at 0 hr and at the end of 3rd hr with a plethysmograph. The percentage of inhibition
of edema by drugs was measured and compared between the test, control and standard groups. In chronic models: Granuloma method was used,
and test drugs were given for 7 days. The animals were divided and treated same as in the acute model. After 7 days treatment, on 8th day animals
were sacrificed each implanted pellet was extracted with adherent granulation tissue. These pellets were dried and weighed. The known weight was
deducted from granulation tissue weight. The mean weight of granulation tissue and difference in weight of granulation tissue for each group was
calculated, and the percentage of inhibition of inflammation was calculated.
Results: The percentage of inhibition of edema in the acute model with 6CH, 300CH after 3 hrs is 51%, 76% respectively and with aspirin is 79%. In
chronic models, the percentage of inhibition of granulation tissue with 6CH, 300CH after 7 days is 19.5%, 32.85%, respectively and with aspirin is
41.95%. The reduction of inflammation was statistically significant in each group is (p<0.001).
Conclusion: Traumeel, a homeo preparation is a best alternative drug for the treatment of acute inflammatory conditions with no side effects.
Keywords: Inflammation, Traumeel preparation, Carrageenan, Aspirin.
Mitchell RN, Cotran RS. In: Robinsons Basic Pathology. 7th ed. New Delhi, India: Harcourt Pvt. Ltd.; 2000. p. 33-42.
Ialenti A, Ianaro A, Moncada S, Di Rosa M. Modulation of acute inflammation by endogenous nitric oxide. Eur J Pharmacol 1992;211(2):177-82.
Di Rosa M, Willoughby DA. Screens for anti-inflammatory drugs. J Pharm Pharmacol 1971;23(4):297-8.
Salvemini D, Wang ZQ, Bourdon DM, Stern MK, Currie MG, Manning PT. Evidence of peroxynitrite involvement in the carrageenan-induced rat paw edema. Eur J Pharmacol 1996;303(3):217-20.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
Ahmad F, Khan RA, Rasheed S. Study of analgesic and anti inflammatory activity from plant extracts of Lactuca scariola and Artemisia absinthium. J Isl Acad Sci 1992;5:111-4.
Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proc Soc Exp Biol Med 1962;111:544-7.
Higgs GA, Moncada S, Vane JR. Eicosanoids in inflammation. Ann Clin Res 1984;16(5-6):287-99.
HernÃ¡ndez-PÃ©rez M, Rabanal RM. Evaluation of the antinflammatory and analgesic activity of Sideritis canariensis var. pannosa in mice. J Ethnopharmacol 2002;81:43-7.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265-75.
Della Loggia R, Tubaro A, Dri P, Zilli C, Del Negro P. The role of flavonoids in the antiinflammatory activity of Chamomilla recutita. Prog Clin Biol Res 1986;213:481-4.
Anderson AJ, Bocklehurst WE, Wills AL. Evidence for the role of lysosomes in the formation of prostaglandins during Carrageenan induced inflammation in rat. Pharmacol Res Commun 1971;3:13-7.
Nair RB, Ravishankar B, Vijayan NP, Sasikala CK, Saraswathy VN. Anti-inflammatory effect of Strbilanthus heyneanus leaves- abiochemical study. J Res Ay Sid 1988;9:46-50.
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