• SAKTHI ABIRAMI M Department of Pharmacology, College of Pharmacy, Madras Medical College, Chennai, Tamil Nadu, India.
  • MUTHUSAMY P Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai, Tamil Nadu, India.



Breast cancer, Polycarpaea corymbosa Lamk, Ehrlich ascites carcinoma, Phytochemistry, Flavonoids, Cancer markers, Membrane transport protein, Nuclei acids, Anticancer


Objective: The current investigation focuses on the study of efficacy of whole plant of Polycarpaea corymbosa Lamk in Ehrlich ascites carcinoma (EAC) inoculated Swiss albino mice.

Methods: The whole plant of P. corymbosa Lamk (WPC) was extracted with solvents of increasing polarity and their percentage yields were calculated. The major phytoconstituents present in the plant extracts were determined by standard chemical tests. Tumor was induced in mice by intraperitoneal injection of EAC cells (1×106 cells/mouse). The in vivo antitumor effect of extracts was assessed by monitoring the mean survival time, tumor volume, effect on hematological parameters, determination of lysosome specific cancer markers (cathepsin-D), β-D glucuronidase and acid phosphatase, liver marker enzymes (5’-nuclotidase and lactate dehydrogenase), membrane bound ATPase (Na+/K+ ATPase and Mg2+ ATPase), DNA, and RNA content.

Results: The percentage yield obtained were 9.87%w/w, 7.88%w/w, and 16.56% w/w for petroleum ether, ethyl acetate, and ethanol extract, respectively. The phytochemical screenings of those extracts were performed. The order of activity of extracts was ethanol extract > ethyl acetate > petroleum ether. Among the extracts, Ethanol extract of P. corymbosa Lamk. showed a significant increase in life span and decrease in viable cancer cell number and tumor volume. The protective effect of the extract on the hemopoietic system at the dose 200mg∕kg was noted. The alterations in the hematological profile, lysosome-specific cancer markers, liver-specific cancer markers, and membrane-bound ATPases DNA and RNA were restored.

Conclusion: The ethanolic extract of P. corymbosa Lamk. possesses in vivo anticancer activity when compared to the tumor control group.


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Rui-Hua X, Pelicano H, Zhou Y, Carew J, Li F, Bhalla KN, et al. Inhibition of glycosides in cancer cells: A novel strategy to overcome drug resistance associated with mitochondrial respiratory defect and hypoxia. Cancer Res 2005;65:613-21.

Perillo B, Di Donato M, Pezone A, Di Zazzo E, Giovannelli P, Galasso G, et al. ROS in cancer therapy: The bright side of the moon. Exp Mol Med 2020;52:192-203.

Jakimiuk K, Wink M, Tomczyk M. Flavonoids of the Caryophyllaceae. Phytochem Rev 2021;2021:1302.

Nadkrarni KM. Indian Materia Medica with Ayurvedic, Unani Tibbi, Siddha, Allopathic, Homeopathic, Naturopathic and Home Remedies, Appendices and Indexes. Vol. 1. Bombay: Popular Prakashan Pvt. Ltd.; 1976. p. 997.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. Vol. 1. New Delhi: CSIR; 1956. p. 199.

Chetty KM, Shivaji K, Rao KT. Polycorpaea corymbosa L, Flowering Plants of Chitoor District, Andrapradesh, India; 2008.

Nishanthini A, Mohan V. Antioxidant activity of Polycarpaea corymbosa L. whole plant of alloxan induced diabetic rats. World J Pharm Pharm Sci 2013;4:343-51.

Nishanthini A, Balamurugan K, Mohan VR. Hepatoprotective and antioxidant effect of Polycarpaea corymbosa against CCl4 induced hepatotoxicity in rats. Int J Adv Life Sci 2012;5:104-11.

Balamurugan K, Devi GS, Mohan VR. In vitro antioxidant activity of whole plant extract of Polycarpaea corymbosa (L.) Lam. (Caryophyllaceae). World J Pharm Pharm Sci 2013;2:3676-90.

Sindhu S, Manorama S. Screening of Polycarpaea corymbosa Lam. (Caryophyllaceae) for its in vitro antioxidant activity. Asian J Pharm Clin Res 2012;5:175-8.

Balamurugan K, Devi GS, Mohan VR. Anti-inflammatory activity of whole plant of Polycarpaea corymbosa Lam. (Caryophyllaceae). Pharm Sci Mon 2012;3:3336-41.

Sindhu S, Manorama S. Anti-inflammatory activity of methanolic extract of Polycarpaea corymbosa Lam. (Caryophyllaceae). Int J Adv Res 2013;1:1-5.

Balamurugan K, Devi GS, Mohan VR. Antiulcer activity of Polycarpaea corymbosa (L.) Lam. whole plant extracts (Caryophyllaceae). Int J Biol Med Res 2013;4:3379-82.

Sindhu S, Manorama S. Study of the anti-ulcerogenic activity of the methanolic extracts of Polycarpaea corymbosa Lam. (Caryophyllaceae) in wistar rats. Int J Pharm Res Schl 2014;3:126-30.

Reddy KY. Acute toxicity and anti-ulcer activity of Polycarpaea corymbosa L. in albino wistar rats. Int J Pharm Pract Drug Res 2012;2:39-45.

Balamurugan K, Devi GS. Anti-diarrhoeal activity of Polycarpaea corymbosa (L.) Lam. whole plant extracts (Caryophyllaceae). J Harmon Res Pharm 2013;2:100-3.

Sindhu S, Manorama S. Antimicrobial activity of Polycarpaea corymbosa Lam. (Caryophyllaceae) against human pathogens. J Chem Pharm Res 2012;4:4014-9.

Sindhu S, Manorama S. Investigating anti nociceptive activity of Polycarpaea corymbosa Lam. (Caryophyllaceae) in standard experimental animal. Int J Pharm Pharm Sci 2014;6:161-3.

Varadharajan R, Rajalingam D. Hepatoprotective effect of Polycarpaea corymbosa L. against CCl4 induced hepatotoxicity in rats. Int J Pharmacother 2012;2:18-23.

Abirami MS, Monika N. Induction of apoptosis in human liver carcinoma cell line and the safety of liver by Polycarpaea corymbosa Lam. J Glob Trends Pharm Sci 2018;9:5861-6.

Mohan VR, Balamurugan K, Devi GS. Fertility enhancement of Polycarpaea corymbosa (L.) Lam (Caryophyllaceae) whole plant on male albino rats. Asian J Pharm Clin Res 2013;6:151-5.

Abirami MS, Muthusamy P. In-vitro cytotoxic activity of various extracts from whole plant of Polycarpaea corymbosa Lam. J Glob Trends Pharm Sci 2016;7:2997-3000.

Prabhavathi RM, Prasad MP, Jayaraman M. Studies on qualitative and quantitative phytochemical analysis of Cissus quadrangularis. Adv Appl Sci Res 2016;7:11-7.

Harborne JB. Phytochemical Methods. 2nd ed. London: Chapman and Hall; 1973. p. 49-188.

OECD 423. OECD Guideline For Testing of Chemicals Acute Oral Toxicity-Acute Toxic Class Method Adopted. Paris: OECD; 2001.

Chandran A, Arunachalam G. Evaluation of in-vivo anticancer activity of Scaevola taccada Roxb. Against Ehrlich Ascites Carcinoma in Swiss albino mice. J Pharm Sci Res 2015;7:626-32.

Mukherjee K. Medical Laboratory Technology a Procedure Manual for Routine Diagnostics Tests. New York: Tata McGraw Hill Publishing Company Limited; 2010.

Sapolsky AI, Atlman RD, Li DS. Cathepsin D activity in normal and osteoarthritic human cartilage. Fed Proc 1973;32:1489-93.

Kawai Y, Anno K. Mucopolysaccharide degrading enzymes from the liver of squid, Ommastrephes slonai pacificus I. Hyaluronidase. Biochem Biophys Acta 1971;242:428-36.

Fiske GH, Subbarow. The calorimetric determination of phosphorus. J Biol Chem 1925;5:375-400.

Heppel LA. Hilmoe RJ. Purification and properties of 5’-nuclotidase. J Biol Chem 1951;188:665-76.

King J. The hydrolases or oxidoreductase, Lactate dehyrogenase. In: Van D, editors. Practical Clinical Enzymology. Kolkata, West Bengal: Norst and Company Ltd.; 1965. p. 83-93.

Bonting SL. In: Bittar EE, editor. Na+- K+ activated Adenosine Triphosphate and Cation Transport in Membrane and Ion Transport. London: Wiley Interscience; 1970. p. 257-363.

Ohinishi T, Suzuki T, Ozawa K. A comparative study of plasma membrane magnesium ion ATPase activities in normal regenerating and malignant cells. Biochem Biophys Acta 1982;684:67-74.

Schneider WC. Phosphorous compounds in animal tissues. Isolation, extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid. J Biol Chem 1945;161:293-303.

Arora A, Byrem TM, Nair MG, Strasburg GM. Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Arch Biochem Biophys 2000;373:102-9.

Novikoff AB, Hers HG, Van Hoff F. Lysosomes and Storage Diseases. Vol. 5. New York: Academic Press; 1973. p. 1-41.

Papac RJ. Origins of cancer therapy. Yale J Biol Med 2001;74:391-8.

Fecchio D, Sirois P, Russo M, Jancar S. Studies on inflammatory response induced by Ehrlich tumor in mice peritoneal cavity. Inflammation 1990;14:125-32.

Motzer RJ, Mazumder M, Bacik J, Berg W. Survival and Prognostic stratification of 670 patients with advanced renal cell carcinoma. J Clin Oncol 1999;17:2530-40.

Fenninger LD, Mider GB. Energy and nitrogen metabolism in cancer. Adv Cancer Res 1954;2:229.

Zein N, Enaiat K, Fatma M, EL Sayed Z. Anti- Proliferative effect of Eucalyptus camaldulensis against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice in vivo. World J Pharm Res 2015;4:272-86.

Fenninger LD, Mider GB. In: Grenstein JP. Haddow A, editors. Advances in Cancer Research. Vol. 2. New York: Academic Press; 1954. p. 244.

Geetha R. Effect of Tocopherol on doxorubicin induced changes in rat heart lysosomal enzymes. Ind J Exp Biol 1993;31:288-90.

Mathieu M, Rochefort H, Barenton B, Prebois C, Vignon F. Interactions of cathepsin-D and insulin like growth factor II (IGF-II) on the IGF-II/mannose -6- phosphate receptor in human breast cancer cells and possible consequences on mitogenic activity of IGF-II. Mol Endocrinl 1990;4:1327-35.

Garcia M, Derocq D, Pujol P, Rochefort H. Over expression of transfected cathepsin-D in transformed cells increases their malignant phenotype and metastatic potency. Oncogene 1990;5:1809-14.

Karunairatnam MC, Kerr LM, Levvy GA. The glucuronide synthesizing system in the mouse and its relationship to β-D-glucuronidase. Biochem J 1949;45:496-9.

Huang XF, Wang CM, Dai XW, Li ZJ, Pan BR, Yu LB, et al. Expression of chromogranin A and cathepsin D in human primary hepatocellular carcinoma. World J Gastroenterol 2000;6:693-8.

Cohen SL, Bittner JJ. The effect of mammary tumors on the glucuronidase and esterase activities in a number of mouse strains. Cancer Res 1951;11:723-6.

De Duve C, Pressman BC, Gianetto R, Wattiaux R, Applemans F. Tissue fractionation studies, Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J 1955;60:604-17.

Matsura SS, Kato EK, Tashiro Y. Ferritin immune electron microscopic localization of 5’ nucleotidase on rat liver cell surface. J Cell Biol1984;99:166-73.

Ayorinde BT, Akanji MA, Yakubu MT. Alterations in some marker enzymes of liver and kidney damage following chronic administration of aqueous extract of Tapinanthus globiferus leaves to rats. Pharmacogn Mag 2008;2008:S9-14.

Veena K, Shanthi P, Sachdanandam P. Anticancer effect of kalpaamruthaa on mammary carcinoma in rats with reference to glycoprotein components, lysosomal and marker enzymes. Biol Pharm Bull 2006;29:565-9.

Walia M, Mahajan M, Singh K. Serum adenosine deaminase, 5’- nucleotidase and alkaline phosphatase in breast cancer patients. Indian J Med Res 1995;101:247-9.

Sunderman FW. The clinical biochemistry of 5’- nucleotidase. Ann Clin Lab Sci 1990;20:123-39.

Suzuki S, Takada T, Sugawara Y, Muto T. Quercetin induces recombinational mutations in cultured cells as detected by DNA finger printing. Jpn J Cancer Res 1991;82:1061-4.

Bioj B, Moreto RD, Farias RN. Membrane lipids fatty acids and regulation of membrane bound enzymes. Biochem Biophys Acta 1973;311:67-9.

Lu A, Gokkuou E, Palandiz O. Vitamin E and ATPases: Protection of ATPase activities by Vitamin E supplementation in various tissues of hypercholesterolemic rats. Int J Vitam Nutr Res 2000;70:3-7.

Skekhoren MA, Bonting Sl. Transport ATPases: Properties and functions. Physiol Rev 1981;61:1-76.

Al-Numair KS, Veeramani C, Alsaif MA, Chandramohan G. Influence of Kaempferol, a flavonoids compound on membrane-bound ATPases in streptozotocin-induced diabetic rats. J Pharm Biol 2014;53:1372-8.

Salmon TB, Evert BA, Song B, Doetsch PW. Biological consequences of oxidative stress induced DNA damage in Saccharomyces cerevisiae. Nucleic Acids Res 2004;32:3712-23.

Das BK, Koti BC, Gadad PC. Role of Lycopersicon esculentum in diethyl nitrosamine induced and phenol barbital promoted hepato cellular carcinoma. Indian J Health Sci Biomed Res 2016;9:147-52.

Lundholm K, Ekman L, Edstrom S, Karlberg I. Protein synthesis in liver tissue under the influence of a methylcholanthrene-induced sarcoma in mice. Cancer Res 1979;39:657-61.

Kumar RS, Kumar SV, Rajkapoor B, Pravin N, Dharmasivam M. Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats. J Appl Pharm Sci 2016;6:199-209.

Modi K, Shah M. Phytochemical investigation and pharmacognostic standardization of Polycarpaea corymbosa Lam. Pharmacogn J 2017;9:895-9.

Sindhu S, Manorama S. GC-MS determination of bioactive components of Polycarpaea corymbosa Lam. (Caryophyllaceae) Hygeia. J D Med 2013;5:5-9.

Manase MJ, Mitaine OA, Miyamoto T, Tanaka C, Delemasure S, Dutartre P, et al. Triterpenoid saponins from Polycarpaea corymbosa Lamk. var. eriantha Hochst. Phytochemistry 2014;100:150-5.

Chiang HC. Studies on the constituents of Polycarpaea corymbosa Lam. Taiwan Yaoxue Zazhi 1978;30:114-20.

Pitchai D, Roy A, Ignatius C. In vitro evaluation of anticancer potentials of lupeol isolated from Elephantopus scaber L. on MCF -7 cell line. J Adv Pharm Technol Res 2014;5:179-84.

Baby TS, Michael BP, Jerard C, Vijayakumar N, Ramachandran R. Study on the antimetastatic and anticancer activity of triterpene compound lupeol in human lung cancer. Int J Pharm Sci Res 2019;10:721-7.

Liu K, Zhang X, Xie L, Deng M, Chen H, Song J, et al. Lupeol and its derivatives as anticancer and anti-inflammatory agents: Molecular mechanisms and therapeutic efficacy. Pharmacol Res 2020;164:105373.

Ayaz M, Sadiq A, Wadood A, Junaid M, Ullah F, Khan Z. Cytotoxicity and molecular docking studies on phytosterols isolated from Polygonum hydropiper L. Steroids 2018;141:30-5.

Ghosh T, Maity TK, Singh J. Evaluation of antitumor activity of stigmasterol, active constituents isolated from Bacopa monneri Linn. Aerial parts against Ehrlich ascites carcinoma in mice. Orient Pharm Exp Med 2011;11:41-9.



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