EFFICACY OF THE SOLVENTS ON THE PHYTOCHEMICAL LOAD AND POLYPHENOL CONTENT OF THE AQUATIC PLANT AZOLLAMICROPHYLLA
Keywords:Azollamicrophylla, Phytochemicals, Soxhlet apparatus, Solvent, Aqueous
Objectives: Azolla microphylla which is an aquatic macrophyte is used to find out efficacy of the different solvents on phytochemical load and polyphenol content. The whole plant was extracted in the Soxhlet apparatus using different solvents such as aqueous, hydro-ethanol, hydro-methanol, and hydro-acetone.
Methods: The four plant extracts were subjected to phytochemical screening by standard procedure. The polyphenol content was estimated in the four extracts using Folin-Ciocalteu method.
Results: The phytochemicals such as flavonoids, phenols, saponins, diterpenes, and protein were present in all the four solvent extracts. The higher proportions of phytochemicals were seen in hydro-methanol extract. The polyphenol content was also higher in the hydro-methanol extract.
Conclusion: The results showed that the extraction of the phytochemicals varied not only plant wise but also from solvent to solvent. The present study revealed the fact that the combination of water and organic solvents gave a better result than aqueous extract.
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