• ADYA CHATURVEDI Department of Zoology, University of Rajasthan, Jaipur, Rajasthan, India.
  • BHAVNA GUPTA Department of Microbiology, Government Medical College, Kota, Rajasthan, India.
  • ASHUTOSH CHATURVEDI Department of ???, Mahatma Gandhi Medical College, Jaipur, Rajasthan, India.
  • RASHMI SISODIYA Department of Zoology, University of Rajasthan, Jaipur, Rajasthan, India.
  • RAJNI SHARMA Department of Microbiology SMS Medical College, Jaipur, Rajasthan, India.



UTI, Risk factors, ESBL, PCDDT, DDAT, E-test, Sensitivity, Specificity


Objective: Globally, urinary tract infections (UTIs) caused by Escherichia coli that produce extended-spectrum lactamase (ESBL) have become more common. Our study determined the magnitude of UTI occurring due to ESBL-producing E. coli and associated risk factors. Different methods for their phenotypic detection were also compared.

Methods: Uropathogenic E. coli isolated in significant numbers were assayed microbiologically. E. coli isolates (n=247) that were found significant in number tested for ESBL production using three different phenotypic methods: Phenotypic combined disk diffusion test (PCDDT), double-disk approximation test (DDAT), and E-test for ESBL production. An antibiotic susceptibility test was performed for different antibiotics. Various risk factors associated with UTIs were correlated with ESBL- and non-ESBL-producing E. coli.

Results: We found that diabetes mellitus type 2 was the most common risk factor for UTI caused due to ESBL-producing E. coli (25%). Pregnant females and patients having recurrent UTI showed less positivity for ESBL production. DDAT detected 32 ESBL-positive isolates and PCDDT detected 37 positive isolates. E-test was taken as the gold standard for ESBL detection which detected 49 isolates as ESBL producers. The highest sensitivity (71.2%) and specificity (75%) were shown by PCDDT.

Conclusion: According to the study conducted, it was concluded that PCDDT was the most reliable and economic method for phenotypic detection of ESBL.


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How to Cite

CHATURVEDI, A., B. GUPTA, A. CHATURVEDI, R. SISODIYA, and R. SHARMA. “STUDY OF MAGNITUDE OF UTI CAUSED BY ESBL-PRODUCING ESCHERICHIA COLI AND ASSOCIATED RISK FACTORS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 15, no. 6, June 2022, pp. 19-21, doi:10.22159/ajpcr.2022.v15i6.43478.



Original Article(s)