TAXONOMIC CHARACTERIZATION, ANTIMICROBIAL ACTIVITY AND ANTIOXIDANT POTENTIAL OF RARE ACTINOBACTERIUM ISOLATED FROM MANGROVE ECOSYSTEM OF MACHILIPATNAM, ANDHRAPRADESH, INDIA
Keywords:Keywords: Mangrove actinomycete, Pseudonocardia parietis VLCH-6, Antimicrobial activity, Antioxidant potential, Bioactive compounds.
Objective: The objective of the present study is to isolate, characterize the potent actinomycete strain from mangrove habitats of Machilipatnam, South coast of Andhra Pradesh, India.
Methods: The samples collected from mangrove habitats were pretreated with calcium carbonate, diluted, plated on yeast extract malt extract dextrose (YMD) agar and Humic acid vitamin (HV) agar media to isolate actinomycetes. The two selective media were supplemented with streptomycin (25 μg/mL) and nystatin (25 μg/mL) to inhibit the growth of bacteria and fungi.
Results: A total of 10 actinobacterial strains were isolated and designated as VLCH-1 to VLCH-10. The isolates were screened and evaluated for their antimicrobial and antioxidant activities. Among them, one strain such as VLCH-6 was found to be potent as it exhibited high antimicrobial potential and it was identified as Pseudonocardia parietis VLCH-6 through polyphasic taxonomy (Genbank accession number: MZ951166). The growth pattern of the isolate was studied by culturing it in YMD broth. The culture broth extracted with ethyl acetate was tested for antimicrobial activity and found to be active against E. coli, X. campestris and A.niger. The antioxidant potential of the strain VLCH-6 was evaluated by DPPH radical scavenging activity. In the screening, the culture extract exhibited strong antioxidant activity against DPPH free radicals.
Conclusion: Over all, it was evident from the present study that mangrove habitats of Machilipatnam serve as a good source for potent actinomycetes possessing broad spectrum of antimicrobial and antioxidant metabolites.
Keywords: Mangrove actinomycete, Pseudonocardia parietis VLCH-6, Antimicrobial activity, Antioxidant potential, Bioactive compounds.
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