TAXONOMIC CHARACTERIZATION, ANTIMICROBIAL ACTIVITY AND ANTIOXIDANT POTENTIAL OF RARE ACTINOBACTERIUM ISOLATED FROM MANGROVE ECOSYSTEM OF MACHILIPATNAM, ANDHRAPRADESH, INDIA

Authors

Keywords:

Keywords: Mangrove actinomycete, Pseudonocardia parietis VLCH-6, Antimicrobial activity, Antioxidant potential, Bioactive compounds.

Abstract

ABSTRACT

Objective: The objective of the present study is to isolate, characterize the potent actinomycete strain from mangrove habitats of Machilipatnam, South coast of Andhra Pradesh, India.

Methods: The samples collected from mangrove habitats were pretreated with calcium carbonate, diluted, plated on yeast extract malt extract dextrose (YMD) agar and Humic acid vitamin (HV) agar media to isolate actinomycetes. The two selective media were supplemented with streptomycin (25 μg/mL) and nystatin (25 μg/mL) to inhibit the growth of bacteria and fungi.

Results: A total of 10 actinobacterial strains were isolated and designated as VLCH-1 to VLCH-10. The isolates were screened and evaluated for their antimicrobial and antioxidant activities. Among them, one strain such as VLCH-6 was found to be potent as it exhibited high antimicrobial potential and it was identified as Pseudonocardia parietis VLCH-6 through polyphasic taxonomy (Genbank accession number: MZ951166). The growth pattern of the isolate was studied by culturing it in YMD broth. The culture broth extracted with ethyl acetate was tested for antimicrobial activity and found to be active against E. coli, X. campestris and A.niger.  The antioxidant potential of the strain VLCH-6 was evaluated by DPPH radical scavenging activity. In the screening, the culture extract exhibited strong antioxidant activity against DPPH free radicals.

Conclusion: Over all, it was evident from the present study that mangrove habitats of Machilipatnam serve as a good source for potent actinomycetes possessing broad spectrum of antimicrobial and antioxidant metabolites.

Keywords: Mangrove actinomycete, Pseudonocardia parietis VLCH-6, Antimicrobial activity, Antioxidant potential, Bioactive compounds.

Downloads

Download data is not yet available.

References

Palla MS, Guntuku GS, Muthyala MKK, Pingali S, Sahu PK. Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil. Beni-Suef University Journal of Basic and Applied Sciences 2018; 7:250-256.

Lu QP, Ye JJ, Huang YM, Liu D, Liu LF, Dong K et al. Exploitation of potentially new antibiotics from mangrove actinobacteria in maowei sea by combination of multiple discovery strategies. Antibiotics 2019; 8:p. 236.

Sharma M. Actinomycetes: Source, identification and their applications. Int J Curr App Sci 2014; 3: 801-832.

Mangamuri U, Muvva V, Poda S, Naragani K, Munaganti RK, Chitturi B et al. Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity. 3 Biotech 2016; 6: 1-8.

Sengupta S, Pramanik A, Ghosh A, Bhattacharyya M. Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem. BMC microbiology 2015; 15: 1-16.

Indupalli MD, Muvva V, Munaganti RK, Mangamuri UK. Saccharomonospora oceani VJDS 3, A potent actinobacterial strain from mangrove ecosystem. Asian J Pharm Clin Res 2016; 9: 363-366.

Bhat SA, Nazir R, Malik TA, Shah FA. Secondary metabolites of actinomycetes as potential source of antibiotics. Stem Cell 2013; 4: 41-46.

Suresh RS, Younis EM, Fredimoses M. Isolation and molecular characterization of novel Streptomyces sp. ACT2 from marine mangrove sediments with antidermatophytic potentials. Journal of King Saud University-Science 2020; 32: 1902-1909.

Mangamuri UK, Muvva V, Poda S, Kamma S. Isolation, identification and molecular characterization of rare actinomycetes from mangrove ecosystem of Nizampatnam. Malaysian Journal of Microbiology 2012; 8: 83-91.

Tanvir R, Sajid I, Hasnain S, Kulik A, Grond S. Rare actinomycetes Nocardia caishijiensis and Pseudonocardia carboxydivorans as endophytes, their bioactivity and metabolites evaluation. Microbiological research 2016; 185: 22-35.

Huang Ying, Liming Wang, Zhitang Lu, Long Hong, Zhihen Liu, Geok Yuan et al. Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia, and description of Pseudonocardia zijingensissp. nov. International journal of systematic and evolutionary microbiology 2002; 52: 977-982.

Kavitha A, Vijayalakshmi M, Sudhakar P, Narasimha G. Screening of actinomycete strains for the production of antifungal metabolites. Afr J Microbiol Res 2011; 4: 27-32.

BSSN HB, Muvva V, Munaganti RK, Naragani K, Konda S, Dorigondla KR. Production of antimicrobial metabolites by Streptomyces lavendulocolor VHB-9 isolated from granite mines. Brazilian Archives of Biology and Technology 2017; 60.

Mogili MS, Muvva V. Selective isolation and characterization of nocardiopsis flavescens vjms-18 from coastal regions of Andhra Pradesh, India. Int J Pharm Res 2019; 10: 1000-08.

Janaki T. Antimicrobial activity of mangrove actinomycetes from soil sample of Rhizophora apiculata. J Biotech Res 2016; 1: 44-52.

Gebreselema G, Feleke M, Samuel S. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethipia. Asian Pac J Trop Biomed 2013; 3: 426-35.

Lakshman M. Application of Conventional Electron Microscopy in Aquatic Animal disease Diagnosis. A review 2019; 7: 470-475.

Mantada PK, Sankar GC, Prabhakar GT. Isolation and characterization of potent antibiotic producing marine actinomycetes from tiruchendur and kulasekarapattinam, tamilnadu. Global J. Sci. Front. Res. 2013; 13: 1-5

Yasmeen S, Vijayalakshmi M, Rajesh Kumar M. Taxonomic characterization of rare actinobacteria isolated from mangrove ecosystem. Int J Curr Research 2016; 8: 31060-31066.

Reddy NG, Ramakrishna DPN, Rajagopal SV. A morphological physiological and biochemical studies of Streptomyces rochei MTCC 10109 showing antagonistic activity against selective human pathogenic microorganisms. Asian J BiolSci 2011; 4: 1-14.

Ragalatha R, Vijayalakshmi M, Mallikarjuna K, Veer Kishore I. Bioactivity and taxonomic studies of Nocardiopsis dassonvillei and Nocardiopsis species from Marine sediments of Bay of bengal, India. Int J Pharm Sci. and Resea.2020; 1: 1790-1801.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 2013; 30: 2725-29.

Jafari N, Behroozi R, Farajzadeh D, Farsi M, Akbari Noghabi K. Antibacterial activity of Pseudonocardia sp. JB05, A rare salty soil actinomycete against Staphylococcus aureus. BioMed Res Int 2014: 1-7.

Kapur MK, Solanki R, Das P, Kumar M, Kumar P. Antimicrobial activity analysis of bioactive compounds from soil actinomycetes. J Pharm Chem BiolSci 2018; 6: 178-187.

Tan LTH, Chan KG, Pusparajah P, Yin WF, Khan TM, Lee LH, et al. Mangrove derived Streptomyces sp. MUM265 as a potential source of antioxidant and anticolon-cancer agents. BMC microbiology 2019; 19: 1-16.

Dini Ryandini, Ocky K Radjasa, Oedjijono. Isolate actinomycetes SA32 origin of Segara Anakan mangrove rhizosphere and its Capability in Inhibiting Multi-Drugs Resistant Bacteria Growth. J Microb Biochem Technol 2018; 10: 1-7.

Govindarajan G, Santhi VS, Jebakumar SRD. Antimicrobial potential of phylogenetically unique actinomycete, Streptomyces sp. JRG-04 from marine origin. Biologicals 2014; 42: 305-311.

Kiranmayi MU, Sudhakar P, Sreenivasulu K, Vijayalakshmi M. Optimization of culturing conditions for improved production of bioactive metabolites by Pseudonocardia sp. VUK-10. Mycobiology 2011; 39: 174-181.

Kavitha A, Vijayalakshmi M. Cultural parameters affecting the production of bioactive metabolites by Nocardia levis MK_VL-113. J Appl Sci Res 2009;5:2138-2147.

Swapna MM and Vijayalakshmi M. Influence of cultural parameters on the production of bioactive metabolites by Streptomyces rectiverticillatus VJMS-8 isolated from south coastal regions of Andhra Pradesh, India. Int J Pharm Sci & Res 2020; 11(10) : 4966-4973.

Published

12-01-2022

How to Cite

CH.CHIRANJEEVI. “TAXONOMIC CHARACTERIZATION, ANTIMICROBIAL ACTIVITY AND ANTIOXIDANT POTENTIAL OF RARE ACTINOBACTERIUM ISOLATED FROM MANGROVE ECOSYSTEM OF MACHILIPATNAM, ANDHRAPRADESH, INDIA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 15, no. 3, Jan. 2022, https://innovareacademics.in/journals/index.php/ajpcr/article/view/43666.

Issue

Section

Original Article(s)