Phytochemical Profiling, HPTLC fingerprint and antibacterial, anti-fungal and antioxidant properties of essential Oils extracted from Cumminum cyminum, Zingiber officinale, Trachyspermum ammi, Alipnia galanga, Cedrus deodara, and Elettaria cardamomum

Phytochemical profiling and bio-activity of essential Oils

Authors

  • Manas Ranjan Sahoo Mr https://orcid.org/0000-0001-8284-7229
  • Guruvaur appan AVN Ayurveda Formulation Pvt Ltd
  • Dr. Ramesh AVN Ayurveda Formulation Pvt Ltd
  • Mrs. Anithakumari AVN Ayurveda Formulation Pvt Ltd
  • Mrs. Maheswari
  • Mr. Ramehs AVN Ayurveda Formulation Pvt Ltd
  • Dr. Srikrishna AVN Ayurveda Formulation Pvt Ltd https://orcid.org/0000-0003-0812-254X

Keywords:

Antimicrobial activity, Bioautography, Essential Oils, Phytochemical analysis, HPTLC fingerprint.

Abstract

Essential oils (EOs) are traditionally used for aromatherapy, pain relief, respiratory infection, skin diseases, and digestive ailments including uses in pharmaceutical, cosmetic and home care applications. In the present study the essential oils extracted from Cumminum cyminum, Zingiber officinale, Trachyspermum ammi, Alipnia galanga, Cedrus deodara, and Elettaria cardamomum were studied. The essential oil was extracted using clavenger apparatus and the HPTLC fingerprint, phytochemical analysis was carried out for the essential oil. The antibacterial and antifungal activity were evaluated using agar well-diffusion method against two bacterial strains, Escherichia coli, Staphylococcus aureus and two fungal strains, Candida albicans, and Aspergillus brasiliensis. Positive controls ciprofloxacin-30mg, azithromycin-15mg and nystatin NS-50mg was used. Antioxidant potential of the essential oils were investigated by TLC-bioautography method using 1,1-diphenyl-2-picrylhydrazyl derivatization. The phytochemical analysis reveals presence of various phytochemical like steroids, terpenoids, phenylpropanoids. The HPTLC fingerprint is found to be unique for each of the oil. The Essential oil of Zingiber officinale and Trachyspermum ammi showed strong antibacterial activity against S.aureus. The essential oils of Cumminum cyminum, Tachyspermum ommi and Alipnia galanga displayed prominent antioxidant activity on TLC bioautography. The herbs Cuminum cynimun, Trachyspermum ammi, Cedrus deodara and Ellateria cardamomum produce reasonable amount of essentials oil, which can be explored for useful their industrial applications. These essential oils can be explored further for their antimicrobial activity. The HPTLC analysis along with derivatization with suitable chromogenic reagents can be an easy and speed tool for quality control of various essential oils.

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Author Biographies

Guruvaur appan , AVN Ayurveda Formulation Pvt Ltd

Pharmaceutcial microbiologist-M.Phil in Microbiology, Officer in QC-microbiology department 

Dr. Ramesh , AVN Ayurveda Formulation Pvt Ltd

Managing Director of the company. Provides the idea about the study and all necessary facilities. 

Mrs. Anithakumari , AVN Ayurveda Formulation Pvt Ltd

Sr. Officer and in R&D-Product development and Herbal API

Mrs. Maheswari

Chemist-API and Product development R&D

Mr. Ramehs , AVN Ayurveda Formulation Pvt Ltd

Chemist-Quality Control 

Dr. Srikrishna, AVN Ayurveda Formulation Pvt Ltd

Manager-in product marketing and clinical trials managment

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Published

13-01-2022

How to Cite

Sahoo, M. R., G. appan . Palanichamy, D. R. R. . Varrier, Rajendran Anithakumari, Harekrishna Maheswari, K Ramesh, and Srikrishna S. “Phytochemical Profiling, HPTLC Fingerprint and Antibacterial, Anti-Fungal and Antioxidant Properties of Essential Oils Extracted from Cumminum Cyminum, Zingiber Officinale, Trachyspermum Ammi, Alipnia Galanga, Cedrus Deodara, and Elettaria Cardamomum: Phytochemical Profiling and Bio-Activity of Essential Oils”. Asian Journal of Pharmaceutical and Clinical Research, vol. 15, no. 3, Jan. 2022, https://innovareacademics.in/journals/index.php/ajpcr/article/view/43737.

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