• ARUNA KUMARI SRRM Government College, Jhunjhunu, Rajasthan, India.
  • R.A. SHARMA Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India.




Ailanthus excelsa, GC-MS, Methanolic extracts, Retention time, Novel drugs


Objective: The objective of the study was to evaluate the phytochemical compounds present in the methanolic fruit extract of Ailanthus excelsa.

Methods: Phytochemical screening was carried out using the GC-MS instrument following the standard protocol.

Results: GC-MS studies revealed the presence of 65 compounds in fruit extract of Ailanthus excelsa. Among them, highest peak area (37.67%) was obtained for Mome Inositol (Six hydroxyl group polysaccharide) (Retention time 14.873).

Conclusion: This study identifies the presence of pharmacologically active compounds which can be constructive for the formulation of novel drugs.


Download data is not yet available.


Qazi MA, Molvi K. Herbal medicine: A comprehensive review. Int J Pharmacol Res 2016;8:1-5.

Adhikari PP, Paul SB. History of Indian traditional medicine: A medical inheritance. Asian J Pharm Clin Res 2018;11:421-6. doi: 10.22159/ ajpcr.2018.v11i1.21893

Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch C, Uhrin P, et al. Discovery and resupply of pharmacologically active plant derived natural products: A review. Biotechnol Adv 2015;33:1582-614. doi: 10.1016/j.biotechadv.2015.08.001, PMID 26281720

Ashraf K, Halim H, Lim SM, Ramasamy K, Sultan S. In vitro antioxidant, antimicrobial and antiproliferative studies of four different extracts of Orthosiphon stamineus, Gynura procumbens and Ficus deltoidea. Saudi J Biol Sci 2020;27:417-32. doi: 10.1016/j. sjbs.2019.11.003, PMID 31889866

Rasouli H, Yarani R, Pociot F, Popović-Djordjević J. Anti-diabetic potential of plant alkaloids: Revisiting current findings and future perspectives. Pharmacol Res 2020;155:104723. doi: 10.1016/j. phrs.2020.104723, PMID 32105756

Maema LP, Potgieter M, Masevhe NA, Samie A. Antimicrobial activity of selected plants against fungal species isolated from South African AIDS patients and their antigonococcal activity. J Complement Integr Med 2020;17:87. doi: 10.1515/jcim-2019-0087, PMID 32301751

Ben-Shabat S, Yarmolinsky L, Porat D, Dahan A. Antiviral effect of phytochemicals from medicinal plants: Applications and drug delivery strategies. Drug Deliv Transl Res 2020;10:354-67. doi: 10.1007/ s13346-019-00691-6, PMID 31788762

Ahmad R, Khan MA, Srivastava AN, Gupta A, Srivastava A, Tanvir R, et al. Anticancer potential of natural dietary products: A comprehensive review. Anti Cancer Agents Med Chem 2020;20:122-236.

Baker DA. Plants against Helicobacter pylori to combat resistance: An ethnopharmacological review. Biotechnol Rep (Amst) 2020;26:00470. doi: 10.1016/j.btre.2020.e00470, PMID 32477900

Islam R, Rahman MS, Rahman SM. GC-MS analysis and antibacterial activity of Cuscuta reflexa against bacterial pathogens. Asian Pac J Trop Dis 2015;5:399-403. doi: 10.1016/S2222-1808(14)60804-5

Adamik K, Brauns FE. Ailanthus glandulosa (tree-of-heaven) as a pulpwood. Tech Asso Pulp 1957;40:522-7.

Jat HS, Singh RK, Mann JS. Ardu (Ailanthus sp.) in arid ecosystem: A compatible species for combating with drought and securing livelihood security of resource poor people. Ind J Trad Knwl 2011;10:102-13.

Sharma M, Sharma N, Srivastava KK, Parmar A. Population dynamics of major insect pests on Ailanthus excelsa Roxb. and their management in arid and semi-arid areas of Rajasthan and Gujarat. Ind Fores 2016;142:900-12.

Lavhale MS, Mishra SH. Nutritional and therapeutic potential of Ailanthus excelsa-a review. Phcog Rev 2007;1:105-13.

Elzaki OT, Khider TO. Strength properties of Ailanthus excelsa Roxb. (tree of heaven) from western Sudan. J Appl Ind Sci 2013;1:38-40.

Dubal KN, Ghorpade PN, Kale MV. Studies on bioactive compounds of Tectaria coadunate. Asian J Pharm Clin Res 2013;6:186-7.

Upadhye A, Kumbhojkar MS, Vartak VD. Observations on wild plants used in folk medicine in the rural areas of the Kolhapur district. Anc Sci Life 1986;6:119-21. PMID 22557559

Cowan MM. Plant products antimicrobial agents. Clin Microbiol Rev 1999;14:564-82.

Ronald Hites A. Gas chromatography-mass spectroscopy. In: Settle FA, editor. Handbook of Instrumental Techniques for Analytical Chemistry. New Jersey: Prentice Hall; 1997. p. 609-24.

Madhavi N, Kumar EA, Maheswar T, Arya N. Standardization of Swasari Vati with reference to gas chromatography, scanning electron microscopy and EDAX. Int J Res Ayurveda Pharm 2014;5:126-31. doi: 10.7897/2277-4343.05226

Jie MS, Choi CY. GC-MS analysis and phytochemical screening of a rare pteridophyte Nephrolepis cardifolia (L.) Presl. from Tirumala Hills. J Int Fed Clin Chem 1991;3:122.

Betz JM, Gay ML, Mossoba MM, Adams S, Portz BS. Chiral gas chromatographic determination of ephedrine type alkaloids in dietary supplements containing Ma Huang. J AOAC Int 1997;80:303-15. doi: 10.1093/jaoac/80.2.303, PMID 9086588

Hassanpouraghdam MB. Flower head volatile oil composition of soil less culture grown Chrysanthemum balsamitta L. Nat Prod Res 2009;23:672-7. doi: 10.1080/14786410802591182, PMID 19401923

Standard Reference Data. National Institute of Standards and Technology (NIST) Mass Database and Software. Version 3.02. United States: Standard Reference Data; 1990.

Lafferly MF. Registry of Mass Spectral Data. 5th ed. New York: Wiley Publishers; 1989.

Sridharan S, Meena V, Kavitha V, Nayagam AA. GC-MS study and phytochemical profiling of Mimosa pudica Linn. J Pharm Res 2011;4:741-2.

Rahman MM, Ahmad SH, Mohamed MT, Ab Rahman MZ. Antimicrobial compounds from leaf extracts of Jatropha curcas, Psidium guajava, and Andrographis paniculata. ScientificWorldJournal 2014;2014:635240.

Das S, Vasudeva N, Sharma S. Chemical composition of ethanol extract of Macrotyloma uniflorum (Lam.) Verdc. using GC-MS spectroscopy. Org Med Chem Lett 2014;4:13. doi: 10.1186/s13588-014-0013-y, PMID 26548989

Hiramoto K, Nasuhara A, Michikoshi K, Kato T, Kikugawa K. DNA strand-breaking activity and mutagenicity of 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one (DDMP), a Maillard reaction product of glucose and glycine. Mutat Res 1997;395:47-56. doi: 10.1016/s1383-5718(97)00141-1, PMID 9465913

Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex nigundo. Afr J Biochem Res 2010;4:191-5.

Yu X, Zhao M, Liu F, Zeng S, Hu J. Identification of 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one as a strong antioxidant in glucose-histidine maillard reaction products. Food Res Int 2013;51:397-403. doi: 10.1016/j.foodres.2012.12.044

Biswas S, Bhattacharyya J, Dutta AG. Oxidant induced injury of erythrocyte-role of green tea leaf and ascorbic acid. Mol Cell Biochem 2005;276:205-10. doi: 10.1007/s11010-005-4062-4, PMID 16132702

Dosek A, Ohno H, Acs Z, Taylor AW, Radak Z. High altitude and oxidative stress. Respir Physiol Neurobiol 2007;158:128-31. doi: 10.1016/j.resp.2007.03.013, PMID 17482529

Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998;46:4113-7. doi: 10.1021/jf9801973

Alamin MA, Samia MA, Alqurashi AM, Elsheikh AS. Bactericidal activity of Psidium guajava leaves against some pathogenic microbes. IOSR J Dent Med Sci 2016;15:61-70.

Dilika F, Bremner PD, Meyer JJ. Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: A plant used during circumcision rites. Fitoterapia 2000;71:450-2. doi: 10.1016/ s0367-326x(00)00150-7, PMID 10925024

Zheng CJ, Yoo JS, Lee TG, Cho HY, Kim YH, Kim WG. Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids. FEBS Lett 2005;579:5157-62. doi: 10.1016/j.febslet.2005.08.028, PMID 16146629

Chandrasekaran M, Senthilkumar A, Venkatesalu V. Antibacterial and antifungal efficacy of fatty acid methyl esters from leaves of Sesuvium portulacastrum L. Eur Rev Med Pharmacol Sci 2011;15:775-80. PMID 21780546

Uraku AJ. GC/MS determination of bioactive constituents of methanol fraction of Spilanthes uliginosa (Sw) leaves. Res J Med Plants 2016;10:42-54. doi: 10.3923/rjmp.2016.42.54

Geetha T, Varalakshmi P. Anti-inflammatory activity of lupeol and lupeol linoleate in rats. J Ethnopharmacol 2001;76:77-80. doi: 10.1016/ s0378-8741(01)00175-1, PMID 11378285

Saleem M. Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett 2009;285:109-15. doi: 10.1016/j. canlet.2009.04.033, PMID 19464787

Poudyali B, Singh B. Potential antimicrobial and antioxidant properties of aqueous, ethanol and methanol extracts of Tectaria macrodonta C. CHR. Int J Pharm Sci Res 2019;10:3785-94.

Bihana S, Dhimin A, Singh G, Satija S. Gas chromatography-mass spectrometry analysis of bioactive compounds in the whole plant parts of ethanolic extract of Asclepias curassavica L. Int J Green Pharm 2018;12:107-14.

Yamuna P, Abirami P, Vijayashalini P, Sharmila M. GC-MS analysis of bioactive compounds in the entire plant parts of ethanolic extract of Gomphrena decumbens Jacq. J Med Plants Stud 2017;5:31-7.

Shah A, Singh T, Vijayvergia R. GC-MS analysis of bioactive phytoconstituents from Rumex vesicarius L. Int Res J Pharm 2015;6:269-72. doi: 10.7897/2230-8407.06459

Socolsky C, Salvatore A, Asakawa Y, Bardon A. Bioactive new bitter testing p-hydroxy styrene glycoside and other constituents from the fern Elaphaglossum spathulatum. Arkivoc 2003;10:347-55.

Wierckx N, Koopman F, Bandounas L, De Winde JH, Ruijssenaars HJ. Isolation and characterization of Cupriavidus basilensis HMF14 for biological removal of inhibitors from lignocellulosic hydrolysate. Microb Biotechnol 2010;3:336-43. doi: 10.1111/j.1751- 7915.2009.00158.x, PMID 21255332

Rajashekar Y, Raghavendra A, Bakthavatsalam N. Acetylcholinesterase inhibition by biofumigant (coumaran) from leaves of Lantana camara in stored grain and household insect pests. BioMed Res Int 2014;2014:187019. doi: 10.1155/2014/187019, PMID 25025036



How to Cite

KUMARI, A., and R. SHARMA. “GC-MS ANALYSIS OF THE METHANOL FRACTION OF AILANTHUS EXCELSA ROXB. FRUIT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 15, no. 6, June 2022, pp. 51-55, doi:10.22159/ajpcr.2022.v15i6.44551.



Original Article(s)