CYTOTOXIC POTENTIAL AND PHYTOCHEMICAL ANALYSIS OF JUSTICIA BEDDOMEI AND ITS ENDOPHYTIC ASPERGILLUS SP
Introduction; Endophytic fungi are mutualistic symbionts of plants producing bioactive secondary metabolites. Objective; The study was carried out to screen for phytochemicals and demonstrate the cyotoxicity, the ethyl acetate extracts of the plant Justicia beddomei and its endophytic fungi. Methods; Preliminary phytochemical screening was carried by standard procedures. The MTT assay of the extracts was carried out on A549 lung adenocarcinoma cells. Results; The endophytic Aspergillus had an IC 50 of 22.73Âµg/ml, while the plant extract had an IC 50 ofÂ Â 6.25Âµg/ml. The phytochemical screening of the extracts showed the presence of phenols, flavonoids, alkaloids, tannins and glycosides. Conclusion; The present study indicates the presence of anticancer compounds in the plant and its endophytic Aspergillus sp. The endophyte was shown to possess bioactivity three times than that of the host plant.
D Wilson, Ecology of woody plant endophytes, in Microbial Endophytes, Marcel Dekker, New York, NY, USA, 2000.
G Strobel and B Daisy,Bioprospecting for microbial endophytes and their natural products .Microbiology and Molecular Biology Reviews 2003;vol 67,no 4:491-502.
A.Stierle,G Strobel and D Stierle,Taxol and taxane production by Taxomyces andreanae,an endophytic fungus of Pacific Yew.Science 1993;vol 260,no5105:214-216.
Sadia Sultan,Syed Adnan Ali Shah,Lin Sun, Kalavathy Ramasami,Anthony Cole,John Blunte et al. Bioactive Fungal metabolites of 9PR2 isolated from roots of Callophyllum ferrugineum. International Journal of Pharmacy and Pharmaceutical Science 2011; Vol 3, Suppl 1: 7-9.
Jianglin Zhao, Yan Mou, Tijiang Shan , Yan Li , Ligang Zhou, Mingan Wang And Jingguo Wang . Antimicrobial Metabolites From The Endophytic Fungus Pichia guilliermondii Isolated From Paris polyphylla var. Yunnanensis,.Molecules 2000; 15; 7961-7970
Tan, R. X. and Zou, W. X.Nat. Prod. Rep. 2001;18:448-459.
Strobe G,Daisy B, Castill U and Harper. Natural products from endophytic microorganisms. Journal of Natural Product. 2004; 67: 257-268.
Srinivasa U, Venkateshwara RJ, Krupanidhi AM, Shivanand K. Antimicrobial activity of Leaves of Justicia beddomei. Indian J Nat Prod 2006;22(3):35.
Jalgaonwala R E, B V Mohite, R T Mahajan. Evaluation of Endophytes for their antimicrobial activity from Indigenous Medicinal Plants belonging to North Maharashtra region India, International Journal on Pharmaceutical and Biomedical Research 2010 ; Vol 1(5):136-141.
Suthep Wiyakrutta, Nongluksna Sriubolmas,Wattana Panphut,Nuntawan Thongon,Kannawat Danwisetkanjana,Nisri Ruangrungsi & Vithaya Meevootisom .Endophytic fungi with antimicrobial, anticancer and antimalarial activities isolated from Thai medicinal plants. World Journal of Microbiology & Biotechnology 2004; 20: 265-272.
Swarnalatha L,Neelakanta Reddy P. Hepatoprotective activity of Sphaeranthus amaranthoides on D-galactosamine induced hepatitis in albino rats.Asian Pacific Journal of Tropical Biomedicine 2012;S1900-S1905.
Maniyar Y,Bhixavatimath P.Evaluation of the hypolycaemic and hypolipidaemic activities of the aqeous extract of the leaves of Ixora coccinea Linn in diabetic rats. J clin Diagn Res 2011;5(7):1381-1384.
M C Alley, D A Scudiero, A Monks, M L Hursey, M J Czerwinski, and D. L Fin.,Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research 1988 ; vol. 48, no. 3: 589â€“601.
LDSette,M.R.ZPassarini,C.Delarmerina,F.Salati,M.C.T.Duarte.Molecular characterisation and antimicrobial activity of endophytic fungi from coffee plants. World J Microbiol Biotechnol 2006;22:1185-1195.
Nameirakpam Nirjanta Devi, John Prabhakaran J , Femina Wahab. Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica.Asian Pacific Journal of Tropical Biomedicine 2012;1-5.
Rashmi Pa,Linu Mathew. Antimicrobial activity of leave extracts of Justicia adhathoda L. in comparison with vasicine. Asian Pacific Journal of Tropical Biomedicine 2012;S1556-S156
Huang W Y, Cai Y Z, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants:Potential use for cancer prevention.Nutr Cancer 2010;62(1):1-20.
Mahendra Rai,Aniket Gade,Dnyaneshwar Rathod,Mudasir Dar,Ajit Varma. Mycoendophytes in medicinal plants:Diversity and bioactivities.Bioscience 2012;Vol 4, No 2:86-96.
Rajesh Kumar Tenguria , Firoz Naem Khan, Sadaf Quereshi. Endophytes-mines of pharmacological therapeutics .World Journal of Science and Technology 2011;1(5):127-149.
Prasetyawan Yunaianto,Syofi Rosmalawati, Indra Rachmavati ,Wahyudi Priyono Suwarso and Wahono Sumaryono. Isolation and Identification of Endophytic fungi from Srikaya Plants (Annona squamosa) having potential secondary metabolites as antibreast cancer activity.Microbiology 2012;Vol 6,No 1:23-29.
R. Vennila, S. V. Thirunavukkarasu, J. Muthumary. In vivo studies on anticancer activity of taxol isolated from an endophytic fungus Pestalotiopsis pauciseta sacc. VM1. Asian Journal of Pharmaceutical and Clinical Reasearch 2010 ; Vol 3 ,issue 4: 30- 34.
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