GC-MS/MS ANALYSIS OF BROWN RICE
Objective: The present study was undertaken to study the phytoconstituents quantitatively through gas chromatography-mass spectrometry
(MS)/MS, as well as its antibacterial, antifungal activities.
Methods: The ethanol extract of brown rice was used for the study. 25gram of brown rice powder was used for the extraction process.
Results: Each peak in the chromatogram represents the signal created by a compound that elutes from the GC column into the detector. The size of
the peaks is proportional to the quantity of the equivalent substances in the specimen analyzed.
The results obtained shows the highest peak for 6-(1-hydroxymethylvinyl)-4, 8a-dimethyl-3, 5, 6, 7, 8, 8a-hexahydro-1H-naphthalen-2one,
26-Nor-5-cholesten-3Î²-ol-25-one, Propane, 1,1-diethoxy-, and stigmastan-3,5-diene. The remaining seven compounds (Stigmasteryl
tosylate, Tetradecanedioic acid, 2H-1-Benzopyran-2-one, 6-acetyl-7-(acetyloxy)-4-methyl-, 3-Isopropoxy-1,1,1,7,7,7-hexamethyl,-3,5,5tris
(trimethylsiloxy) tetrasiloxane, Dodecanoic acid, ethyl ester, t-Butyl hydrogen phthalate) showed moderate levels of a peak area percent except
3, 4-Dihydroxy-Î±-(isopropylaminomethyl)-benzyl alcohol (isoproterenol) as it shows only 1.73 as peak area percent, which was found to be low
compared to other compounds identified. No compound was present in trace amount. The range of retention time falls within the range of 4.79
Conclusion: The antimicrobial property could be attributed due to the phytochemicals present in brown rice. Essential oil, sterols and various other
bioactive constituents present in it would have been responsible for the antibacterial, antifungal activity. Brown rice is a whole grain which could be
germinated to improve its overall health benefits. Brown rice extract finds applications in pharmaceutical preparations.
Keywords: Antibacterial, Antifungal, Brown rice, Gas chromatography-mass spectrometry/mass spectrometry.
therapy in Type 2 diabetes mellitus. Asian J Pharm Clinical Res
2. Bauer AW, Perry DM, Kirby WM. Single-disk antibiotic-sensitivity
testing of staphylococci; an analysis of technique and results. AMA
Arch Intern Med 1959;104(2):208-16.
3. Krishnaveni M, Dhanalakshmi R. Phytoconstituent study of brown rice.
World J Pharm Res 2014;3(8):1092-9.
4. Krishnaveni M, Magesh P, George JS, Dhanalakshmi R, Ponraj K,
Lavanya K, et al. Antioxidant potential of brown rice. Int J Pharm Sci
Rev Res 2014;28(1):134-7.
5. Madari H, Panda D, Wilson L and Jacobs RS. Dicoumarol: unique
microtubule stabilizing natural product that is synergistic with Taxol.
Cancer Res, 2003; 63(6): 1214-20.
6. Kostova I, Synthetic and natural coumarins as cytotoxic agents, Curr
Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 76-79
Med Chem Anticancer Agents 2005; 5(1): 9-46.
7. Takeuchi Y, Xie L, Cosentino LM and Lee KH. Anti-AIDS agents-
XXVIII. Synthesis and anti-HIV activity of methoxy substituted 3â€™,4â€™di-
Chem Lett 1997; 7: 2573-8.
8. Shikishima Y, Takaishi Y, Honda G, Ito M, Takfda Y, Kodzhimatov O K,
Ashurmetov O, Lee KH. Chemical constituents of Prangos
tschiniganica; structure elucidation and absolute configuration of
coumarin and furanocoumarinderivatives with anti-HIV activity. Chem
Pharm Bull 2001; 49(7): 877-80.
9. Manolov I, Maichle-Moessmer C and Danchev N. Synthesis,
structure,toxicological and pharmacological investigations of
4-hydroxycoumarin derivatives. Eur J Med Chem 2006; 41(7): 882-90.
10. Jung J, Kin J and Park O. Simple and cost effective syntheses of 4
hydroxycoumari n. Synth.Commun 1999; 29: 3587-95.
11. Ostrov DA, Hernandez Prada JA, Corsino PE, Finton KA, Le Nand
Rowe TC. Discovery of novel DNA gyrase inhibitors by highthroughput
12. Musiciki B, Periers AM, Laurin P, Ferroud D, Benedetti Y,Lachaud S,
Chatreaux F, Haesslein JL, Ltis A, Pierre C, KhiderJ, Tessol N,
Airault M, Demassey J, Dupuis-Hamelin C, Lassaigne P, Bonnefoy A,
Vicat P and Klich M. Improved antibacterial activities of coumarin
antibiotics bearing 5â€™,5â€™-dialkylnoviose: biological activity of RU
79115. Bioorg Med Chem Lett. 2000; 10: 1695-9.
13. Koshy L, Dwarakanath BS, Raj HG, Chandra R and Mathew TL.
Suicidal oxidative stress induced by certainantioxidants. Indian J Exp
Biol. 2003; 41(11): 1273-8.
14. Fylaktakidou KC, Hadjipavlou-Litina DJ, Litinas KE and
Nicolaides DN. Natural and synthetic coumarin derivatives with
anti-inflammatory/antioxidant activities. Curr Pharm Des. 2004;10(30):
15. Ghate M, Manohar D, Kulkarni V, Shobha R and Kattimani SY.
Synthesis of vanillin ethers from 4-(bromomethyl) coumarins asantiinflammatory
agents. Eur J Med Chem, 2003; 38(3): 297- 302.
16. Kontogiorgis CA, Hadjipavlou-Litina DJ. Synthesis and anti
inflammatory activity of coumarin derivatives, J Med Chem. 2005;
17. Partridge JE, Shannon LM, Gumpf DJ, Colbaugh P. Glycoprotein in the
capsid of plant viruses as a possible determinant of seed transmissibility.
Nature 1974; 247: 391-2.
18. Khripach VA, Zhabinskii VN, Konstantinova OV, Antonchick AP,
Schneider B. Synthesis of 26-2H(3) brassinosteroids. Steroids 2002;
19. Boligon AA, Janovik, V, Frohlich JK, Spader TB, Froeder ALF,
Alves SH, Athayde ML. Antimicrobial and cytotoxic activities of
leaves, twigs and stem bark of Scutia buxifolia Reissek. Nat. Prod. Res.
2012; 26: 939-44.
20. Tg Siti Amirah Tg Kamazeri, Othaman Abd Samah, Muhammad
Taher, Deny Susanti, Haitham Qaralleh. Antimicrobial activity and
essential oils of Curcuma aeuroginosa, Curcuma mangga, and Zingiber
cassumunar from Malaysia. Asian pacific journal of Tropical Medicine
21. Meenakshi VK, Gomathy S. Chamundeswari KP. GC-MS analysis of
the simple ascidian Microcosmus exasperatus Heller 1878. Inter. J.
ChemTech. Res. 2012; 4: 55-62.
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