• CHELLARAM C Vel Tech Multitech
  • Alex John A


Objective: To screen and isolate peptide antibiotics producing epibiotic bacteria from the surface of the sea fan Juncella juncea, and partially purify
the active compound.
Methods: In this study, epibiotic bacteria were isolated by swabbing a small area of the coral surface with a sterile cotton swab, which was then
directly swabbed on to Zobell marine agar plates. Antibiotic production was screened using agar overlay method and the active peptide was partially
purified using anion exchange chromatography and reversed phase high-performance liquid chromatography (RP-HPLC). Phylogenetic identification
based on comparative sequence analysis of 16Ss rRNA gene indicated that the stain fell under the genera Streptomyces.
Results: In the initial screening using agar overlay method the strain was found to exhibit broad spectral activity exhibiting both antibacterial and
antifungal activities. Protease digestion of the crude extract resulted in the loss of activity indicating the proteinaceous nature of the active molecules.
Bioassay guided purification using anion exchange chromatography, and RP-HPLC was carried and the active peptide was partially purified. An
epibiotic Streptomyces strain CN3 producing potent broad spectral peptide was isolated.
Conclusion: This study highlights the importance of epibiotic bacteria associated with corals as a potential source for the discovery of novel
antimicrobials and other natural products.
Keywords: Coral, Matrix-assisted laser desorption/ionization time-of-flight, Selected pathogens.

Author Biography

CHELLARAM C, Vel Tech Multitech



1. Wahl M. Goeche F, Labes A, Dobretsov S, Weinberger F. The second skin: Ecological role of epibiotic biofilms on marine organisms. Front Microbiol 2012;3:192-292.
2. Steinberg PD, Rice SA, Campbell AH, McDougald D, Harder T. Interfaces between bacterial and eukaryotic “neuroecology.” Integr Comp Biol 2011;51(5):794-806.
3. Chellaram C, Anand TP. Antagonistic effect of hard coral associated bacteria from Tuticorin coastal waters. Int J Pharm Pharm Sci 2012;4 Suppl 1:580-3.
4. Chellaram C, Anand TP, Shailaja, NR, Kesavan D. Herbicidal effects of marine animal, Trochus tentorium from gulf of Mannar, Southeastern India. Asian J Anim Vet Adv 2012;7(3):250-5.
5. Burja AM, Hill RT. Microbial symbionts of the Ausralian great barrier reef sponge, Candida spongia flabellate. Hydrobiologia 2001;461:41-7.
6. Hentschel U, Fieseler L, Wehrl M, Gernert C, Steinert M, Hacker J, et al. Microbial diversity of marine sponges. In: Müller WE, editor. Marine Molecular Biotechnology. Berlin: Springer - Verlag; 2003. p. 59-88.
7. Webster NS, Taylor MW. Marine sponges and their microbial symbionts: Love and other relationships. Environ Microbiol 2012;14(2):335-46.
8. Chellaram, C, Edward JK. Anti-inflammatory potential of coral reef associated gastropod, Drupa margariticola. Indian J Sci Technol 2009a;2(2):75-7.
9. Jensen PR, Fenical W. Strategies for the discovery of secondary metabolites from marine bacteria: Ecological perspectives. Annu Rev Microbiol 1994;48:559-84.
10. Boyd KG, Mearns-Spragg A, Burgess JG. Screening of marine bacteria for the production of microbial repellents using a spectrophotometric chemotaxis assay. Mar Biotechnol (NY) 1999;1(4):359-63.
11. Lemos ML, Toranzo AE, Barja JL. Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds. Microb Ecol 1985;11(2):149‑63.
12. Burgess JG, Jordan EM, Bregu M, Mearns-Spragg A, Boyd KG. Microbial antagonism: A neglected avenue of natural products research. J Biotechnol 1999;70(1-3):27-32.
13. Chellaram C, Anand TP, Kesavan D, Priya G, Chandrika M, Gladis C. Enhanced cultivability of antagonistic bacterial strains from soft coral Sinularia sp., Gulf of Mannar, Southeastern India. Afr J Microbiol Res 2011;5(12):1521-6.
14. Chellaram C, Edward JK. Antimicrobial activity of the marine Mollusk Nassa costata from the Tuticorin coastal waters, Southeastern India. Asian J Microbiol Biotechnol Environ Sci 2009;11(1):41-8.
15. Schubert PF, Finn RK. Alcohol precipitation of proteins: The relationship of denaturation and precipitation of catalase. Biotechnol Bioeng 1981;23:2569-90.
16. Pidiyar VJ, Jangid K, Dayananda KM, Kaznowski A, Gonzalez JM, Patole MS, et al. Phylogenetic affiliation of Aeromonas culicicola MTCC 3249(T) based on gyrB gene sequence and PCR-amplicon sequence analysis of cytolytic enterotoxin gene. Syst Appl Microbiol 2003;26(2):197-202.
17. Renner MK, Shen YC, Cheng XC, Jensen PR, Frankmoelle W, Kauffman CA, et al. Cyclomarins A-C, new anti inflammatory cyclic peptides produced by a marine bacterium (Streptomyces sp.). J Am Chem Soc 1999;121:11273-6.
18. Nam SJ, Kauffman CA, Jensen PR, Fenical W. Isolation and characterization of actinoramides A-C, highly modified peptides from a Marine Streptomyces sp. Tetrahedron 2011;67(35):6707-12.
19. Anand TP, Chellaram C, Mark Praveen M, Guru GS, Shanthini FC. Herbicidal and growth promoter activity of three saltmarsh plants from Pulikat coast. Int J Chem Tech Res 2014;6(2):1051-4.
20. Chellaram C. Structural elucidation of a chemical compound from mollusk, Drupa margariticola against fish pathogens. Res J Biotechnol 2014;9(6):52-8.
21. Valli S, Suvathi SS, Aysha OS, Nirmala P, Vinoth KP, Reena A. Antimicrobial potential of Actinomycetes species isolated from marine environment. Asian Pac J Trop Biomed 2012;2(6):469-73.
22. Selvakumar D, Arun K, Suguna S, Kumar D, Dhevendaran K. Bioactive potential of Streptomyces against fish and shellfish pathogens. Iran J Microbiol 2010;2(3):157-64.
23. Hu Y, MacMillan JB. A new peptide isolated from a marine derived Streptomyces bacillaris. Nat Prod Commun 2012;7(2):211-4.
24. Chellaram C, Jonesh S, Anand TP, Edward JK. Insecticidal bustle of corallivorous gastropod, Drupa margariticola. J Biol Sci 2009;9(7):784‑7.
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How to Cite
C, C., and A. John A. “BIOACTIVE POTENTIAL OF AN EPIBIOTIC STREPTOMYCES STRAIN, CN3 FROM THE SEA FAN JUNCELLA JUNCEA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 4, July 2015, pp. 188-91,
Original Article(s)