PHARMACOLOGICAL SCREENING FOR POTENCY OF ANTIOBESITY ON FIVE PLANTS BASED ON ETHNOPHARMACOLOGICAL USE
Objective: The objective of this research was to screening potency of anti-obesity effect and antioxidant activity from Sapindus rarak Pulp., Punica
granatum Leaves, Jasminllum sambac Flos., Tamarindus indica Pulp., and Senna alexandrina Leaves.
Methods: This study was performed through in-vitro method to determine the potency of anti-obesity, such as its ability to inhibit pancreatic lipase
activity and radical-scavenging activity against free radicals like 1,1-diphenyl-2-picrylhydrazyl to determine the potency of these plants as antioxidant.
Results: The 50% inhibitory concentration of pancreatic lipase activity inhibitory and antioxidant activity consecutively, P. granatum Leaves
(20.64 ppm; 77.89 ppm), J. sambac Flos. (53.46 ppm; 127.95 ppm), T. indica Pulp. (62.02 ppm; 159.16 ppm), S. alexandrina Leaves (94.69 ppm;
142.28 ppm), and S. rarak Pulp. (796.32 ppm; 147.56 ppm).
Conclusion: P. granatum Leaves showed the best potency of anti-obesity compared to other plants based on its ability to inhibit pancreatic lipase
activity and antioxidant activity.
Keywords: Anti-obesity, Pancreatic lipase activity, Antioxidant.
1. Han LK, Zheng YN, Yoshikawa M, Okuda H, Kimura Y. Anti-obesity effects of chikusetsusaponins isolated from Panax japonicus rhizomes. BMC Complement Altern Med 2005;5:9.
2. Huttunen R, SyrjÃ¤nen J. Obesity and the risk and outcome of infection. Int J Obes (Lond) 2013;37:333-40.
3. Earthman CP, Beckman LM, Masodkar K, Sibley SD. The link between obesity and low circulating 25-hydroxyvitamin D concentrations: Considerations and implications. Int J Obes (Lond) 2012;36(3):387-96.
4. Patel RM, Patel NJ. In vitro antioxidant activity of coumarin compounds by DPPH, super oxide and nitric oxide free radical scavenging methods. J Adv Pharm Edu Res 2011;1:52-68.
5. Han LK, Kimura Y, Okuda H. Reduction in fat storage during chitin-chitosan treatment in mice fed a high-fat diet. Int J Obes 1999;23(2):174â€‘9.
6. Chaturvedi S, Joshi A, Dubey BK. Pharmacognostical, phytochemical, and cardioprotective activity of Tamarindus indica Linn. Bark. Int J Pharm Sci Res 2011;2(11):3019-27.
7. Pradono DI, Darusman LK, Susanti A. Pancreatic lipase in vitro inhibition by aqueous and ethanol extract of asam jawa leaves (Tamarindus indica) and kunci pepet rhizome (Kaempferiae rotundae). J Nat Indones 2011;13(2):146-54.
8. Rani N, Vasudeva N, Sharma SK. Quality assessment and anti-obesity activity of Stellaria media (Linn.) Vill. BMC Complement Altern Med 2012;12:145.
9. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, et al. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 2004;114(12):1752-61.
10. Lee H, Lee YJ, Choi H, Ko EH, Kim JW. Reactive oxygen species facilitate adipocyte differentiation by accelerating mitotic clonal expansion. J Biol Chem 2009;284(16):10601-9.
11. Higuchi M, Dusting GJ, Peshavariya H, Jiang F, Hsiao ST, Chan EC, et al. Differentiation of human adipose-derived stem cells into fat involves reactive oxygen species and Forkhead box O1 mediated upregulation of antioxidant enzymes. Stem Cells Dev 2013;22(6):878â€‘88.
12. Sfar S, Boussoffara R, Sfar MT, Kerkeni A. Antioxidant enzymes activities in obese Tunisian children. Nutr J 2013;12:18.
13. Savini I, Catani MV, Evangelista D, Gasperi V, Avigliano L. Obesity-associated oxidative stress: Strategies finalized to improve redox state. Int J Mol Sci 2013;14(5):10497-538.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.