• Mahendra Shivshankar Khyade Department of Botany, S. N. Arts, D. J. M. Commerce and B.N. S. Science College, Sangamner- 422605, Maharashtra, India. Affiliated to Savitribai Phule Pune University (formerly University of Pune).
  • Suresh P. Kamble
  • Anil R. Kurhe


Objective: To evaluate the comparative Fourier Transform Infrared spectroscopic analysis and the in vitro antioxidant screening of leaf extract of
three Cassia species.
Methods: The 80% leaf methanol extract of all three species was tested for their total phenols, flavonoids, antioxidant screening (DPPH, ABTS, Nitric
oxide, radical reducing power and total antioxidant capacity) and along with spectroscopic study.
Results: The results revealed that C. uniflora had the highest total phenolic content (276.54±0.031 µg/ mg DW) followed by C. occidentalis and C. tora,
while the highest total flavonoid content (43.71±0.086 µg /mg DW) was also recorded to C. uniflora followed by C. tora and C. occidentalis. FTIR
screening revealed the presence of various functional groups such as aldehydes, alcohols, carboxylic acid, alkenes, aromatic compounds, esters, nitro
compounds and alkyl halides in all the three species. Overall all the species exhibited dose dependent antioxidant activity in tested in vitro models.
The antioxidant activity was varied to each species; the highest IC50 values for DPPH (58.55±0.213 μg/ml) were depicted in C. occidentalis followed
by C. tora and C. uniflora. The prominent IC50 values for ABTS radical scavenging was reported in C. uniflora extract (IC50value of 0.074±0.003 μg/ml)
followed by C. occidentalis and C. tora (with IC50 values of 24.388±0.183 and 29.388±0.368 μg/ml equivalent to ascorbic acid).
Conclusion: The results of the present study indicate that, of the Cassia species evaluated, C. occidentalis exhibited strong activity followed by C. tora
and C. uniflora, and can be used as a potent source of natural antioxidants.
Keywords: Cassia species, Leaf extract, Fourier transform infrared spectroscopy, Antioxidant activity, Ascorbic acid.



1. El-Hashash MM, Abdel-Gawad MM, El-Sayed MM, Sabry WA,
Abdel-Hameed el-SS, Abdel-Lateef Eel-S. Antioxidant properties of
methanolic extracts of the leaves of seven Egyptian Cassia species.
Acta Pharm 2010;60:361-7.
2. Anonymous. The Wealth of India. Raw Materials. Vol. II. Delhi: CSIR;
3. Ganapaty S, Thomas PS, Ramana KV, Vidyadhar K, Chakradhar VA.
Review of phytochemical studies of Cassia species. J Nat Remedies
4. Liogier HA. Descriptive Flora of Puerto Rico and Adjacent Islands.
Spermatophyla. Vol. 2. Rio Piedras, PR: Editorial de la Universidad de
Puerto Rico; 1988. p. 481.
5. Stevens WD, Ulloa UC, Pool A, Monitel OH. Flora de Nicaragua.
Monographs of Systematic Botany, 85. St. Louis, MO: Missouri
Botanical Garden Press; 2001.
6. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. US:
Springer; 2007.
7. Kirtikar KR, Basu BD. Indian Medicinal Plant. Allahabad: Allahabad
Lalit Mohan Basu; 1933.
8. Nadkarni AK. Indian Materia Medica. Bombay: Popular Publication;
9. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants.
New Delhi: CSIR; 1980.
10. Warrier PK, Nambiar VP. Indian Medicinal Plants: A Compendium of
500 Species. Hyderabad: Orient Orient Longman Ltd.; 1994.
11. Patil MV, Patil DA. Ethnobotany of Nasik District of Maharashtra.
New Delhi: Daya Books; 2006.
12. Pardo de Tavera TH. The Medicinal Plants of the Philippines.
Philadelphia: P. Blakiston’s Son & Co.; 2008.
13. Duke JA. Duke’s Handbook of Medicinal Plants of Latin America.
Boca Raton: CRC Press Taylor & Francis Group; 2009.
14. Coimbra R. Manual de Fitoterapia. Belém, Brazil: Editora CEJUP;
15. Di Stasi LC, Hiruma - Rasp AC. Plants Medicine on Amazônia and on
Forest Atlantic. Publishing company UNESP São Paul; 2002 p.604.
16. Silva MG, Aragão TP, Vasconcelos CF, Ferreira PA, Andrade BA,
Costa IM, et al. Acute and subacute toxicity of Cassia occidentalis L.
stem and leaf in Wistar rats. J Ethnopharmacol 20112;136(2):341-6.
17. Daniel M. Medicinal Plants: Chemistry and Properties. Hauppauge, NY,
USA: Scientific Publishers; 2005. p. 175.
18. Rai PM, Shok M. Anthraquinone glycosides from plant parts of Cassia
occidentalis. Indian J Pharm Sci 1983;45:87-8.
19. Tiwari RD, Singh J. Anthraquinone pigments from cassia occidentalis.
Planta Med 1977;32(4):375-7.
20. Tiwar RD, Singh J. Flavonoids from the leaves of Cassia occidentalis.
Phytochemistry 1977;16:1107-8.
21. Hussain HS, Deeni YY. Plants in Kano ethnomedicine: Screening for
antimicrobial activity and alkaloids. Int J Pharmacogn 1991;29:51-6.
22. Muanza DN, Dangala NL, Mpay O. Zairean medicinal plants as
diarrhea remedies and their antibacterial activities. Afr Study Monogr
23. Ali MS, Azhar I, Amtul Z, Ahmad VU, Usmanghani K. Antimicrobial
screening of some Caesalpiniaceae. Fitoterapia 1999;70:299-304.
24. Tona L, Ngimbi NP, Tsakala M, Mesia K, Cimanga K, Apers S,
et al. Antimalarial activity of 20 crude extracts from nine African
medicinal plants used in Kinshasa, Congo. J Ethnopharmacol
25. Tona L, Cimanga RK, Mesia K, Musuamba CT, De Bruyne T, Apers S,
et al. In vitro antiplasmodial activity of extracts and fractions from
seven medicinal plants used in the democratic republic of Congo.
J Ethnopharmacol 2004;93(1):27-32.
26. Sadique J, Chandra T, Thenmozhi V, Elango V. Biochemical modes
of action of Cassia occidentalis and Cardiospermum halicacabum in
inflammation. J Ethnopharmacol 1987;19(2):201-12.
27. Arya V, Yadav JP. Antioxidant properties of the methanol extracts of
the leaves seeds and stem of Cassia occidentalis. Res J Med Plants
28. Sheeba Rani M, Emmanuel S, Sreekanth MR, Ignacimuthu S.
Evaluation of in vivo antioxidant and hepatoprotective activity of
Cassia occidentalis L. against paracetamol induced liver toxicity in
rats. Int J Pharm Pharm Sci 2010;2:67-70.
29. Jain S, Patil UK. Phytochemical and pharmacological profile of Cassia
tora L. - An overview. Indian J Nat Prod Resour 2010;1:430-7.
30. Rejiya CS, Cibin TR, Abraham A. Leaves of Cassia tora as a novel cancer
therapeutic – An in vitro study. Toxicol In Vitro 2009;23(6):1034-8.
31. Daiziel AJ. The Useful Plants of West Tropical Africa. London: Crown
Agents for Overseas Governments and Administrations; 1995.
32. Irvine FR. Woody Plants of Ghana. London: Oxford University Press;
33. Chidume FC, Kwanashie HO, Adekeye JO, Wambebe C,
Gamaniel KS. Antinociceptive and smooth muscle contracting activities
of the methanolic extract of Cassia tora leaf. J Ethnopharmacol
34. WHO. Medicinal Plants of Vietnam. Manila: World Health
Organization; 1989.
35. Maity TK, Mandal SC, Mukharjee PK, Saha K, Das J, Pal M, et al.
Studies on Anti-inflammatory effect of Cassia tora leaf extract
(Fam.- Leguminosae). Phytother Res 1998;12:221-3.
36. Maity TK, Mandal SC, Mukharjee PK, Saha K, Das J, Pal M, et al.
Evaluation of hepatoprotective potential of Cassia tora leaf extract. Nat
Prod Sci 1997;3(2):122-6.
37. Dhanasekaran M, Ignacimuthu S, Agastian P. Potential hepatoprotective
activity of ononitol monohydrate isolated from Cassia tora L. on carbon
tetrachloride induced hepatotoxicity in wistar rats. Phytomedicine
38. Chidume FC, Kwanashie HO, Adekeye JO, Wambebe C,
Gamaniel KS. Antinociceptive and smooth muscle contracting activities
of the methanolic extract of Cassia tora leaf. J Ethnopharmacol
39. John J, Mehta A, Mehta P. Evaluation of antioxidant and anticancer
potential of Cassia tora leaves. Asian J Tradit Med 2012;7:260-7.
40. Mukherjee PK. Quality Control of Herbal Drug. New Delhi: Business
Horizons; 2002.
41. Amala S. Traditional Medicines for Modern Times. USA: Taylor and
Francis; 2005.
42. Yun-Choi HS, Kim JH, Takido M. Potential inhibitors of platelet
aggregation from plant sources, V. Anthraquinones from seeds of Cassia
obtusifolia and related compounds. J Nat Prod 1990;53(3):630-3.
43. Shibata S, Morishita E, Kaneda M, Kimura Y, Takido M, Takahashi S.
Chemical studies on the oriental plant drugs. XX. The constituents
of Cassia tora L 1. The structure of torachrysone. Chem Pharm Bull
(Tokyo) 1969;17(3):454-7.
44. Cooke T. The Flora of the Presidency of Bombay. Calcutta: BSI, 1955.
45. Pradhan SG, Singh NP. Flora of Ahmednagar District (M.S). Dehradun,
India: Bishen Singh Mahendra Pal Singh; 1999.
46. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques
of Plant Analysis. 3
ed. London: Academic Press; 1998.
47. Mahida Y, Mohan JS. Screening of Indian plant extracts for antibacterial
activity. Pharm Biol 2006;44(8):627-31.
48. Trease GE, Evans WC. Pharmacognosy. 12
ed. London: Baillieere
Tindal; 1983.
49. Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid-rich
extract of Hypericum perforatum L. in vitro. J Agric Food Chem
50. Cliffe S, Fawer M, Maier G, Takata K, Ritter G. Enzyme assays for the
phenolic content of natural juices. J Agri Food Chem 1994;42:1824-8.
51. Janakiraman N, Sahaya SS, Johnson M. UV-VIS and FTIR spectroscopic
studies on Peristrophe bicalyculata (Retz.) Nees. Asian J Pharm Clin
Res 2011;4(4):125-9.
52. Basavegowda N, Idhayadhulla A, Lee YR. Phyto-synthesis of gold
nanoparticles using fruit extract of Hovenia dulcis and their biological
activities. Ind Crops Prod. 2014;52:745-51.
53. Yen GC, Chen HY. Antioxidant activity of various tea extracts in
relation to their anti-mutagenicity. J Agric Food Chem 1995;43:27-32.
54. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M,
Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 121-127
Khyade et al.
Rice-Evans C. Antioxidant activity applying an improved ABTS radical
cation decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.
55. Ilavarasan R, Mallika M, Venkataraman S. Anti-inflammatory and
antioxidant activites of Cassia fistula Linn bark extract. Afr J Tradit
56. Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: Direct
measure of total antioxidant activity of biological fluids and modified
version for simultaneous measurement of total antioxidant power and
ascorbic acid concentration. Methods Enzymol 1999;299:15-27.
57. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of
antioxidant capacity through the formation of a phosphomolybdenum
complex: Specific application to the determination of vitamin E. Anal
Biochem 1999;269(2):337-41.
58. Shirwaikar A, Rajendran K, Kumar CD. In vitro antioxidant studies of
Annona squamosa Linn. leaves. Indian J Exp Biol 2004;42(8):803-7.
59. Olajide OA, Aderogba MA, Adedapo AD, Makinde JM. Effects of
Anacardium occidentale stem bark extract on in vivo inflammatory
models. J Ethnopharmacol 2004;95(2-3):139-42.
60. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and
inhibition of intercellular communication by antioxidant catechins
isolated from Chinese green tea. Carcinogenesis 1989;10(6):1003-8.
61. Ogunleye DS, Ibitoye SF. Studies of antimicrobial activity and
chemical constituents of Ximenia americana. Trop J Pharm Res
62. Decorti RC, Creacy WA. In: Dekker M, Taylor W, Fanworths NR.
The Cantharadus Alkaloids. New York: Taylor W and Fanworths NR
Publishers; 1975. p. 237-78.
63. Oloyede KG, Oke MJ, Raji Y, Olugbade AT. Antioxidant and
anticonvulsant alkaloids in Crinum ornatum bulb extract. World J
Chem 2010;5:26-31.
64. Cheeke PP. Surprising benefits of desert plants. Corvallis, Oregon:
Oregon University, in the Linus Paulin Institute; 2005.
65. Mu L, Kou J, Zhu D, Yu B. Comparison of neuroprotective effects of
flavonoids, terpenoids, and their combinations from Ginkgo biloba on
ischemia-reperfusion-injured mice. Pharm Biol 2007;45:728-33.
66. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in
selected herbs. J Agric Food Chem 2001;49(11):5165-70.
67. Arya V, Yadav JP. Antioxidant activity and total phenolics in leaves
extracts of Cassia Tora L. Pharmacol Online 2010;2:1030-6.
68. Arya V, Yadav JP. Comparative assessment of relative antioxidant
activity of sequential leaf extracts of Cassia occidentalis L. and C. Tora
L. Pharmacol Online 2011;1:529-43.
69. Ksouri R, Megdiche W, Falleh H, Trabelsi N, Boulaaba M, Smaoui A,
et al. Influence of biological, environmental and technical factors on
phenolic content and antioxidant activities of Tunisian halophytes. C R
Biol 2008;331(11):865-73.
70. Khoudja NK, Makhlouf LB, Madani K. Antioxidant capacity of crude
extracts and their solvent fractions of selected Algerian lamiaceae. Ind
Crops Prod 2014;52:177-82.
71. Ismail HI, Wei CK, Mariod AA, Ismail M. Phenolic content and
antioxidant activity of cantaloupe (Cucumis melo) methanolic extracts.
Food Chem 2010;119:643-7.
72. Wang SS, Wang DM, Pu WJ, Li DW. Phytochemical profiles,
antioxidant and antimicrobial activities of three Potentilla species.
BMC Complement Altern Med 2013;13:321.
73. Apak R, Güçlü K, Demirata B, Ozyürek M, Celik SE, Bektasoglu B,
et al. Comparative evaluation of various total antioxidant capacity
assays applied to phenolic compounds with the CUPRAC assay.
Molecules 2007;12(7):1496-547.
74. Cotelle N, Bernier JL, Catteau JP, Pommery J, Wallet JC, Gaydou EM.
Antioxidant properties of hydroxy-flavones. Free Radic Biol Med
75. Sathya AV, Ambikapathy V, Panneer Selvam A. Studies on the
phytochemistry, antimicrobial activity and antioxidant properties of
Cassia occidentalis L. Asian J Plant Sci Res 2012;2(4):530-3.
76. Gbadegesin MA, Odunola OA. In vitro antioxidant/radical scavenging
activities and hepatoprotective roles of ethanolic extract of Cassia
occidentalis leaves in sodium arsenite-treated male wistar rats. Br J
Med Med Res 2013;3:2141-56.
77. Sirappuselvi S, Chitra M. In vitro antioxidant activity of Cassia tora
Lin. Int Res J Biol Sci 2012;1:57-61.
78. John J, Mehta A, Mehta P. Evaluation of antioxidant and anticancer
potential of Cassia tora leaves. Asian J Tradit Med 2012;7(6):260-7.
79. Sahu RK, Kar M, Routray R. DPPH Free radical scavenging activity
of some leafy vegetables used by tribals of Odisha. India. J Med Plants
Stud 2013;1(4):21-7.
80. Kim DO, Lee KW, Lee HJ, Lee CY. Vitamin C equivalent antioxidant
capacity (VCEAC) of phenolic phytochemicals. J Agric Food Chem
81. Gouveia S, Gonc alves J, Castilho PC. Characterization of phenolic
compounds and antioxidant activity of ethanolic extracts from flowers
of Andryala glandulosa ssp. varia (Lowe ex DC.) R. Fern., an endemic
species of Macaronesia region. Ind Crops Prod 2013;42:573-82.
82. Xi W, Zhang Y, Sun Y, Shen Y, Ye X, Zhou Z. Phenolic composition
of Chinese wild mandarin (Citrus reticulate Balnco.) pulps and their
antioxidant properties. Ind Crops Prod 2014;52:466-74.
83. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative
properties of xanthone on the auto oxidation of soybean in cylcodextrin
emulsion. J Agric Food Chem 1992;40:945-8.
84. Dorman HJ, Peltoketo A, Hiltunen R, Tikkanen MJ. Characterisation
of the antioxidant properties of deodourisation aqueous extracts from
selected Lamiaceae Herbs. Food Chem 2003;83:255-6.
85. Contreras-Calderón J, Calderón-Jaimes L, Guerra-Hernández E,
García-Villanova B. Antioxidant capacity, phenolic content and
vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia.
Food Res Int 2011;44:2047-53.
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How to Cite
Khyade, M. S., S. P. Kamble, and A. R. Kurhe. “COMPARATIVE FTIR ANALYSIS AND FREE RADICAL QUENCHING PROPERTIES OF THREE CASSIA SPECIES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 119-25,
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