• Boominathan Meena Department of Zoology, Presidency College (Autonomous), Chennai - 600 005.
  • Ayothi Suresh Department of Zoology, Presidency College (Autonomous), Chennai - 600 005.
  • Rose Sumit Department of Zoology, Presidency College (Autonomous), Chennai - 600 005.
  • Ramalingam Mani Department of Biotechnology, St.Peter's University, Avadi, Chennai - 54.


Objectives: Metallothioneins (MTs) have been widely considered for their potential use as a specific biomarkers to reflect the existence of heavy
metal pollution, because their induction has been observed to be obviously elevated after heavy metal exposure in a large number aquatic
organisms. However, relatively fewer efforts have been made to study the related effects of MT in fish species, such as Ctenopharyngodon idella
(C. idella), a globally important aquaculture species. The objective of this study was to determine the cadmium (Cd) levels and MT induction in
muscle, gill, kidney, and liver in C. idella during acute Cd exposure, to study the relationship between tissue-specific Cd accumulation and MT
Methods: Cd accumulation and MT induction levels was determined according to the methods of Ma et al., 2007. Cd concentration was determined
using an atomic absorption spectrophotometer (Perkin Elmer Optima-5300 DV).
Results: The MT induction levels were found in an increasing order of liver>kidney>gill >muscle. A positive correlation was shown between MT
induction and Cd accumulation. The accumulation of Cd levels in all tissues is distinct by time-dependent and dose-dependent.
Conclusion: These results suggest that MT in the liver played an important role to detoxify high quantities of Cd. Cd accumulation showed a positive
correlation with MT induction in all the tissues studied. Hence, the present investigation marks that MT levels can be considered as a biomarker for
acute waterborne Cd.
Keywords: Heavy metals, Atomic absorption spectrophotometry, Biomarker protein, Metallothionein, Ctenopharyngodon idella.


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How to Cite
Meena, B., A. Suresh, R. Sumit, and R. Mani. “INDUCTION OF METALLOTHIONEIN WITH CADMIUM CHLORIDE IN A ECONOMICALLY IMPORTANT FRESHWATER FISH-GRASS CARP, CTENOPHARYNGODON IDELLA (VALENCIENNES, 1844)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 276-81,
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