INDUCTION OF METALLOTHIONEIN WITH CADMIUM CHLORIDE IN A ECONOMICALLY IMPORTANT FRESHWATER FISH-GRASS CARP, CTENOPHARYNGODON IDELLA (VALENCIENNES, 1844)

  • Boominathan Meena Department of Zoology, Presidency College (Autonomous), Chennai - 600 005.
  • Ayothi Suresh Department of Zoology, Presidency College (Autonomous), Chennai - 600 005.
  • Rose Sumit Department of Zoology, Presidency College (Autonomous), Chennai - 600 005.
  • Ramalingam Mani Department of Biotechnology, St.Peter's University, Avadi, Chennai - 54.

Abstract

Objectives: Metallothioneins (MTs) have been widely considered for their potential use as a specific biomarkers to reflect the existence of heavy
metal pollution, because their induction has been observed to be obviously elevated after heavy metal exposure in a large number aquatic
organisms. However, relatively fewer efforts have been made to study the related effects of MT in fish species, such as Ctenopharyngodon idella
(C. idella), a globally important aquaculture species. The objective of this study was to determine the cadmium (Cd) levels and MT induction in
muscle, gill, kidney, and liver in C. idella during acute Cd exposure, to study the relationship between tissue-specific Cd accumulation and MT
induction.
Methods: Cd accumulation and MT induction levels was determined according to the methods of Ma et al., 2007. Cd concentration was determined
using an atomic absorption spectrophotometer (Perkin Elmer Optima-5300 DV).
Results: The MT induction levels were found in an increasing order of liver>kidney>gill >muscle. A positive correlation was shown between MT
induction and Cd accumulation. The accumulation of Cd levels in all tissues is distinct by time-dependent and dose-dependent.
Conclusion: These results suggest that MT in the liver played an important role to detoxify high quantities of Cd. Cd accumulation showed a positive
correlation with MT induction in all the tissues studied. Hence, the present investigation marks that MT levels can be considered as a biomarker for
acute waterborne Cd.
Keywords: Heavy metals, Atomic absorption spectrophotometry, Biomarker protein, Metallothionein, Ctenopharyngodon idella.

References

1. Jabeen G. Studies on fish species specific metals bio-accumulation
patterns in relation to water, sediments and plankton in the river Ravi.
Ph.D. Thesis. Department of Zoology and Fisheries University of
Agriculture Faisalabad; 2012.
2. Azmat H, Javed M, Jabeen G. Acute toxicity of aluminium to the
fish (Catla catla, Labeo rohita and Cirrhina mrigala). Pak Vet
2012;31(1):85-7.
3. Jones MM, Cherian C. Cadmium a unique metal. Toxicol
1990;62(1):1-25.
4. Otchere FA. Heavy metals concentrations and burden in the bivalves
(Anadara (Senilia) senilis, Crassostrea tulipa and Pernaperna) from
lagoons in Ghana: Model to describe mechanism of accumulation/
excretion. Afr J Biotech 2003;2(9):280-7.
5. Amisah S, Adjei-Boateng D, Obirikorang KA, Quagrainie K. Effects of
clam size on heavy metal accumulation in whole soft tissues of Galatea
paradoxa (Born, 1778) from the Volta estuary, Ghana. Int J Fish Aquat
2009;1:014–21.
6. Ahmed MS, Aslam Y, Khan WA. Absorption and bioaccumulation of
water-borne inorganic mercury (Hg) in the fingerlings of grass carp,
Ctenopharyngodon idella. J Anim Plant Sci 2011;21(2):176-81.
7. Roberts JR. Metal toxicity in children. Training Manual on Pediatric
Environmental Health: Putting it into Practice. Emeryville, CA:
Children’s Environmental Health Network; 1999.
8. Järup L. Hazards of heavy metal contamination. Br Med Bull
2003;68:167-82.
9. Bryan GW, Langston WJ. Bioavailability, accumulation and effects of
heavy metals in sediments with special reference to United Kingdom
estuaries: A review. Environ Pollut 1992;76(2):89-131.
10. Atrno K, Volker R, Yona C. Cadmium binding by fractions of dissolved
organic matter and humic substances from municipal solid waste
compost. J Environ Qual 2002:31(6):1885-92.
11. Audry S, Schäfer J, Blanc G, Jouanneau JM. 50-year sedimentary
record of heavy metal pollution (Cd, Zn, Cu, Pb) in the Lot River
reservoirs (France). Environ Pollut 2004;132(3):413-26.
12. Chrastný V, Komárek M, Tlustos P, Svehla J. Effects of flooding on lead
and cadmium speciation in sediments from a drinking water reservoir.
Environ Monit Assess 2006;118(1-3):113-23.
13. Waisberg M, Joseph P, Hale B, Beyersmann D. Molecular and
cellular mechanisms of cadmium carcinogenesis. Toxicology
2003;192(2-3):95-117.
14. Margoshes M, Vallee BL. A cadmium protein from equine imaging
hyperintensity in Alzheimer’s disease: Correlation with kidney cortex.
J Am Chem Soc 1957;79:4813-4.
15. Syring RA, Brouwer TH, Brouwer M. Cloning and sequencing of
cDNAs encoding for a novel copper-specific metallothionein and two
cadmium-inducible metallothioneins from the blue crab Callinectes
sapidus. Comp Biochem Physiol C 2000;125(3):325-32.
16. Kägi JH, Schäffer A. Biochemistry of metallothionein. Biochemistry
1988;27(23):8509-15.
17. Sorensen EM. Cadmium. Metal Poisoning in Fish. Boston: CRC Press;
1991. p. 175-234.
18. Ma W, Wang L, He Y, Yan Y. Tissue-specific cadmium and
metallothionein levels in Sinopotamon henanense. Environ Toxicol
2007;23(3):393-400.
19. Pedersen KL, Pedersen SN, Højrup P, Andersen JS, Roepstorff P,
Knudsen J, et al. Purification and characterization of a cadmiuminduced
metallothionein
from
the
shore
crab
Carcinus
maenas
(L.).

Biochem
J 1994;297:609-14.
20. Jezierska B, Witeska M. Metal Toxicity to Fish. Siedlce, Poland:
University of Podlasie; 2001. p. 51-68.
21. Kay J, Thomas DG, Brown MW, Cryer A, Shurben D, Solbe JF,
et al. Cadmium accumulation and protein binding patterns in tissues
of the rainbow trout, Salmo gairdneri. Environ Health Perspect
1986;65:133-9.
22. Giles MA. Accumulation of cadmium by rainbow trout, Salmo gairdneri,
during extended exposure. Can J Fish Aquat Sci 1988;45:1045-53.
23. Glynn AW, Olsson PE. Cadmium turnover in minnows (Phoxinus
phoxinus) preexposed to water born cadmium. Environ Toxicol Chem
1991;10(3):338-94.
24. Gill TS, Epple A. Stress-related changes in the hematological profile
of the American eel (Anguilla rostrata). Ecotoxicol Environ Saf
1993;25(2):227-35.
25. Martinez M, Torreblanca A, Ramo JD, Pastor A, Diaz-Mayans J.
Cadmium induced metallothionein in hepatopancreas of Procambarus
clarkii: Quantification by a silver-saturation method. Comp Biochem
Physiol C 1993;105(2):263-7.
26. Wu JP, Chen HC. Metallothionein induction and heavy metal
accumulation in white shrimp Litopenaeus vannamei exposed to
cadmium and zinc. Comp Biochem Physiol C 2005;140(3-4):383-94.
136
Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 132-137
Suresh et al.
27. Hogstrand C, Haux C. Binding and detoxification of heavy metals in
lower vertebrates with reference to metallothionein. Comp Biochem
Physiol C 1991;100(1-2):137-41.
28. Kovarova J, Kizek R, Adam V, Harustiakova D, Celechovska O,
Svobodova Z. Effect of cadmium chloride on metallothionein levels in
carp. Sensors (Basel) 2009;9(6):4789-803.
29. Berntssen MH, Aspholm OO, Hylland K, Wendelaar-Bonga SE,
Lundebye AK. Tissue metallothionein, apoptosis and cell proliferation
responses in Atlantic salmon (Salmo salar L.) parr fed elevated dietary
cadmium. Comp Biochem Physiol C 2001;128(3):299-310.
30. Huang ZY, Zhang Q, Chen J, Zhuang ZX, Wang XR. Bioaccumulation
of metals and induction of metallothioneins in selected tissues
of common carp (Cyprinus carpio). Appl Organomet Chem
2007;21(2):101-7.
Statistics
134 Views | 493 Downloads
How to Cite
Meena, B., A. Suresh, R. Sumit, and R. Mani. “INDUCTION OF METALLOTHIONEIN WITH CADMIUM CHLORIDE IN A ECONOMICALLY IMPORTANT FRESHWATER FISH-GRASS CARP, CTENOPHARYNGODON IDELLA (VALENCIENNES, 1844)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 276-81, https://innovareacademics.in/journals/index.php/ajpcr/article/view/6941.
Section
Original Article(s)