IN VITRO ANTICANCER POTENTIAL OF STATIN FROM ASPERGILLUS TAMARII GRD119
Objectives: Fungi have been largely targeted for their biopotential mainly in pharmaceutical industry. Recently, there has been an increase in screening of biopharmaceuticals from microorganisms so as to eliminate side-effects and chemical damages from non-natural sources.
Methods: In the present study, Aspergillus tamarii GRD119 (JX110981) was screened for the production of statin by conducting bioassays against Candida and filamentous fungi.
Results: Based on the results obtained showing effective inhibition potential against Candida spp and A. fumigatus, further analysis of purification of statin by several chromatographic analyses such as column chromatography, TLC, HPTLC and HPLC was conducted. The extracted statin shows strong cytotoxic activity against HEP G2 cell line.
Conclusions: These results designate the fungal strain and the statin produced can serve as candidates for potential genetic transformation and further structural analysis respectively.
Keywords: Aspergillus tamarii, chromatography, purification, statin, cytotoxicity.
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