PHYTOCHEMICAL SCREENING, FREE RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITY OF CROTON LEPTOSTACHYUS KUNTH LEAF EXTRACTS.

  • Wilmer Sánchez Universidad del Tolima
  • Luis Fernando Ospina Universidad Nacional de Colombia
  • Claudia Cristina Pérez Universidad del Tolima
  • Walter Arango Murillo Universidad del Tolima
  • Jonh Jairo Mendez Universidad del Tolima

Abstract

Objective: To study the biological potential of Croton leptostachyus Kunth (Mosquero) leaves through phytochemical screening and determination of the free radical scavenging capacity (against DPPH. and ABTS.+) and anti-inflammatory activity via a TPA induced auricular edema model in ICR mice of the crude ethanolic extract and subextracts (of n-hexane, ethyl acetate and ethanol).

Materials and methods: The C. lepstostachyus leaf extracts were obtained via ethanolic maceration and subsequent partition of the dry ethanolic crude extract with n-hexane and ethyl acetate, the aforementioned samples were tested for phytochemical composition, antiradical activity (DPPH. and ABTS.+), and anti-inflammatory activity (TPA induced auricular edema model in mice).

Results: The phytochemical plant profile showed a diversity of secondary metabolites such as terpenic compounds (triterpenes and saponins), phenolics (flavonoids, tannins and phenylpropanoids), alkaloids and a minor proportion of iridoids and coumarins. Free radical scavenging assays indicated a presumable combined effect of the C. leptostachyus leaf metabolites considering the fact that the crude extract was the most active scavenger of DPPH. and ABTS.+. However, between the subextracts, the ethanolic subextract showed the highest antiradical activity, indicating a presumable major contribution of phenolic compounds in this result. The topical anti-inflammatory action of ethyl acetate subextract showed a possible enhanced effect due to a higher concentration of medium polarity compounds;  no anti-inflammatory activity of the subsequent fractions of this sample was observed,  indicating  a probably interaction of the metabolites contained therein.

Conclusion: C. leptostachyus leaf extracts showed potential as free radical scavengers and anti-inflammatory compounds sources.

Keywords: Croton leptostachyus, Free radical scavenging activity, Anti-inflammatory activity.

Author Biographies

Wilmer Sánchez, Universidad del Tolima
Rersearcher, Grupo de Investigación en Productos Naturales-GIPRONUT.  Universidad del Tolima.
Luis Fernando Ospina, Universidad Nacional de Colombia
Professor, Bioterium Director, Departament of Pharmacy. Universidad Nacional de Colombia.
Claudia Cristina Pérez, Universidad del Tolima
Rersearcher, Grupo de Investigación en Productos Naturales-GIPRONUT.  Universidad del Tolima.
Walter Arango Murillo, Universidad del Tolima

Professor, Chemistry Department, Universidad del Tolima

Researcher, Grupo de Investigación en Productos Naturales-GIPRONUT, Universidad del Tolima

 

Jonh Jairo Mendez, Universidad del Tolima

Professor, Chemistry Department, Universidad del Tolima

Director, Grupo de Investigación en Productos Naturales-GIPRONUT, Universidad del Tolima

 

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How to Cite
Sánchez, W., L. F. Ospina, PérezC. C., W. A. Murillo, and J. J. Mendez. “PHYTOCHEMICAL SCREENING, FREE RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITY OF CROTON LEPTOSTACHYUS KUNTH LEAF EXTRACTS.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 190-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/7236.
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