• Wilmer Sánchez Universidad del Tolima
  • Luis Fernando Ospina Universidad Nacional de Colombia
  • Claudia Cristina Pérez Universidad del Tolima
  • Walter Arango Murillo Universidad del Tolima
  • Jonh Jairo Mendez Universidad del Tolima


Objective: To study the biological potential of Croton leptostachyus Kunth (Mosquero) leaves through phytochemical screening and determination of the free radical scavenging capacity (against DPPH. and ABTS.+) and anti-inflammatory activity via a TPA induced auricular edema model in ICR mice of the crude ethanolic extract and subextracts (of n-hexane, ethyl acetate and ethanol).

Materials and methods: The C. lepstostachyus leaf extracts were obtained via ethanolic maceration and subsequent partition of the dry ethanolic crude extract with n-hexane and ethyl acetate, the aforementioned samples were tested for phytochemical composition, antiradical activity (DPPH. and ABTS.+), and anti-inflammatory activity (TPA induced auricular edema model in mice).

Results: The phytochemical plant profile showed a diversity of secondary metabolites such as terpenic compounds (triterpenes and saponins), phenolics (flavonoids, tannins and phenylpropanoids), alkaloids and a minor proportion of iridoids and coumarins. Free radical scavenging assays indicated a presumable combined effect of the C. leptostachyus leaf metabolites considering the fact that the crude extract was the most active scavenger of DPPH. and ABTS.+. However, between the subextracts, the ethanolic subextract showed the highest antiradical activity, indicating a presumable major contribution of phenolic compounds in this result. The topical anti-inflammatory action of ethyl acetate subextract showed a possible enhanced effect due to a higher concentration of medium polarity compounds;  no anti-inflammatory activity of the subsequent fractions of this sample was observed,  indicating  a probably interaction of the metabolites contained therein.

Conclusion: C. leptostachyus leaf extracts showed potential as free radical scavengers and anti-inflammatory compounds sources.

Keywords: Croton leptostachyus, Free radical scavenging activity, Anti-inflammatory activity.

Author Biographies

Wilmer Sánchez, Universidad del Tolima
Rersearcher, Grupo de Investigación en Productos Naturales-GIPRONUT.  Universidad del Tolima.
Luis Fernando Ospina, Universidad Nacional de Colombia
Professor, Bioterium Director, Departament of Pharmacy. Universidad Nacional de Colombia.
Claudia Cristina Pérez, Universidad del Tolima
Rersearcher, Grupo de Investigación en Productos Naturales-GIPRONUT.  Universidad del Tolima.
Walter Arango Murillo, Universidad del Tolima

Professor, Chemistry Department, Universidad del Tolima

Researcher, Grupo de Investigación en Productos Naturales-GIPRONUT, Universidad del Tolima


Jonh Jairo Mendez, Universidad del Tolima

Professor, Chemistry Department, Universidad del Tolima

Director, Grupo de Investigación en Productos Naturales-GIPRONUT, Universidad del Tolima



1. Okechukwu PN, Ekeuku S. Inhibition of arachidonic acid-induced ear
edema, mast-cell stabilizing and free radical effect of dichloromethane
crude extracts from the leaves of Labisia pumila. Asian J Pharm Clin
Res 2013;1(1):93-5.
2. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress,
inflammation, and cancer: How are they linked? Free Radic Biol Med
3. Lloyd CM, Saglani S. T cells in asthma: Influences of genetics,
environment, and T-cell plasticity. J Allergy Clin Immunol
4. Falconer CL, Cooper AR, Walhin JP, Thompson D, Page AS,
Peters TJ, et al. Sedentary time and markers of inflammation in people
with newly diagnosed type 2 diabetes. Nutr Metab Cardiovasc Dis
5. Ferreira ST, Clarke JR, Bomfim TR, De Felice FG. Inflammation,
defective insulin signaling, and neuronal dysfunction in Alzheimer’s
disease. Alzheimers Dement 2014;10 1 Suppl: S76-83.
6. Bowie A, O’Neill LA. Oxidative stress and nuclear factor-kappaB
activation: A reassessment of the evidence in the light of recent
discoveries. Biochem Pharmacol 2000;59(1):13-23.
7. Echeverri R, Nancy P, Mockus S. Factor nuclear κB (NF-κB):
Signalosoma y su importancia en enfermedades inflamatorias y cáncer.
Rev Fac Med Univ Nac Colomb 2008;56(2):133-46.
8. López-Bojorquez LN. Regulation of NF-kappaB transcription factor.
A molecular mediator in inflammatory process. Rev Invest Clin
9. Winrow VR, Winyard PG, Morris CJ, Blake DR. Free radicals in
inflammation: Second messengers and mediators of tissue destruction.
Br Med Bull 1993;49(3):506-22.
10. Kristián T, Siesjö BK. Calcium in ischemic cell death. Stroke
11. Martínez CD, Vargas CR, Arancibia SR. Oxidative stress and
Neurodegeneration. Rev Fac Med 2003;46(6):229-35.
12. Céspedes Miranda E, Castillo Herrera J. Lipid peroxidation in diagnosis
of oxidative stress of hypertensive patient. Rev Cubana Invest Bioméd
13. Cracowski JL. Isoprostanes: An emerging role in vascular physiology
and disease? Chem Phys Lipids 2004;128(1-2):75-83.
14. Galati G, Tafazoli S, Sabzevari O, Chan TS, O’Brien PJ. Idiosyncratic
NSAID drug induced oxidative stress. Chem Biol Interact
15. McAnulty SR, Owens JT, McAnulty LS, Nieman DC, Morrow JD,
Dumke CL, et al. Ibuprofen use during extreme exercise: Effects on
oxidative stress and PGE2. Med Sci Sports Exerc 2007;39(7):1075-9.
16. Adão CR, da Silva BP, Parente JP. A new steroidal saponin with
antiinflammatory and antiulcerogenic properties from the bulbs of
Allium ampeloprasum var. porrum. Fitoterapia 2011;82(8):1175-80.
17. Arawwawala LD, Arambewela LS, Ratnasooriya WD. Alpinia
calcarata Roscoe: A potent antiinflammatory agent. J Ethnopharmacol
18. Otuki MF, Vieira-Lima F, Malheiros A, Yunes RA, Calixto JB.
Topical antiinflammatory effects of the ether extract from Protium
kleinii and alpha-amyrin pentacyclic triterpene. Eur J Pharmacol
19. Salatino A, Salatino ML, Negri G. Traditional uses, chemistry and
pharmacology of Croton species (Euphorbiaceae). J Braz Chem Soc
20. Bingtao LI, Huaxing Q, Jin-Shuang M, Hua Z, Michael G, Gea JH.
Flora of China. Beijing: Missouri Botanical Garden Press; 2008.
21. Murillo J. Euphorbiaceae of Colombia. Biota Colomb
22. Caruzo MB, van Ee BW, Cordeiro I, Berry PE, Riina R. Molecular
Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 198-202
Peralta et al.
phylogenetics and character evolution of the “sacaca” clade: Novel
relationships of Croton section Cleodora (Euphorbiaceae). Mol
Phylogenet Evol 2011;60(2):193-206.
23. De Marino S, Gala F, Zollo F, Vitalini S, Fico G, Visioli F, et al.
Identification of minor secondary metabolites from the latex of Croton
lechleri (Muell-Arg) and evaluation of their antioxidant activity.
Molecules 2008;13(6):1219-29.
24. Khadabadi S, Baviskar B, Khangenbam R, Koli U, Daga N, Gadbail P,
et al. In vitro antioxidant activity and phenolic content of Croton
caudatum. In Vitro 2009;1(2):174-6.
25. Nardi GM, Felippi R, DalBó S, Siqueira-Junior JM, Arruda DC, Delle
Monache F, et al. Anti-inflammatory and antioxidant effects of Croton
celtidifolius bark. Phytomedicine 2003;10(2-3):176-84.
26. Nardi GM, Siqueira Jr JM, Delle Monache F, Pizzolatti MG, Ckless K,
Ribeiro-do-Valle RM. Antioxidant and anti-inflammatory effects of
products from Croton celtidifolius Bailon on carrageenan-induced
pleurisy in rats. Phytomedicine 2007;14(2):115-22.
27. Suárez AI, Compagnone RS, Salazar-Bookaman MM, Tillett S, Delle
Monache F, Di Giulio C, et al. Antinociceptive and anti-inflammatory
effects of Croton malambo bark aqueous extract. J Ethnopharmacol
28. Suárez AI, Blanco Z, Compagnone RS, Salazar-Bookaman MM, Zapata
V, Alvarado C. Anti-inflammatory activity of Croton cuneatus aqueous
extract. J Ethnopharmacol 2006;105(1-2):99-101.
29. Schultes RE. Members of euphorbiaceae in primitive and advanced
societies. Bot J Linn Soc 1987;94(1-2):79-95.
30. Barbosa PS, Abreu AdS, Batista EF, Guilhon GM, Müller AH, Arruda
MS, et al. Glutarimide alkaloids and terpenoids from Croton pullei var.
Glabrior Lanj. Biochem Syst Ecol 2007;35(12):887-90.
31. del Castillo Cotillo H, Simone FD, Feo VD. Proaporphine alkaloids
from Croton ruizianus Muell.-Arg. (Euphorbiaceae). Biochem Syst
Ecol (United Kingdom) 1996;24(5): 463-64.
32. Bhakuni D, Satish S, Dhar M. The alkaloids of Croton sparsiflorus.
Phytochemistry 1970;9(12):2573-80.
33. Amaral AC, Barnes RA. A tetrahydroprotoberberine alkaloid from
Croton hemiargyreus. Phytochemistry 1998;47(7):1445-7.
34. González-Vázquez R, King Díaz B, Aguilar MI, Diego N, LotinaHennsen
B. Pachypodol from Croton
Ort. as
enzyme inhibitor on thylakoids. J
Food Chem
35. Krebs HC, Ramiarantsoa H. Clerodane diterpenes of Croton hovarum.
Phytochemistry 1997;45(2):379-81.
36. Athikomkulchai S, Prawat H, Thasana N, Ruangrungsi N, Ruchirawat
S. COX-1, COX-2 inhibitors and antifungal agents from Croton
hutchinsonianus. Chem Pharm Bull (Tokyo) 2006;54(2):262-4.
37. Pardo Rodriguez DA, Ortíz Romero LT, Tacha AL, Murillo Perea
E, Mendez Arteaga JJ, Murillo Arango W. Phytochemical and
ethnobotanical study of Croton leptostachyus. Rev Acad Colomb Cienc
Exactas Físicas Nat 2014;38(149):356-63.
38. Hort MA, Straliotto MR, Duz MS, Netto PM, Souza CB, Schulz T,
et al. Cardioprotective effects of a proanthocyanidin-rich fraction
from Croton celtidifolius Baill: Focus on atherosclerosis. Food Chem
Toxicol 2012;50(10):3769-75.
39. Kolak U, Oztürk M, Ozgökçe F, Ulubelen A. Norditerpene alkaloids
from Delphinium linearilobum and antioxidant activity. Phytochemistry
40. Chau VM, Phan VK, Nguyen X, Nguyen XC, Nguyen PT, Nguyen HN,
et al. Cytotoxic and antioxidant activities of diterpenes and sterols from
the Vietnamese soft coral Lobophytum compactum. Bioorg Med Chem
Lett 2011;21:2155-9.
41. Domínguez XA. Métodos de Investigación Fitoquímica. México DF:
Limusa; 1973.
42. Braca A, Sortino C, Politi M, Morelli I, Mendez J. Antioxidant
activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol
43. Kuskoski EM, Asuero AG, García-Parilla MC, Troncoso AM, Fett R.
Actividad antioxidante de pigmentos antociánicos. Food Sci Technol
(Campinas) 2004;24(4):691-3.
44. De Young LM, Kheifets JB, Ballaron SJ, Young JM. Edema and cell
infiltration in the phorbol ester-treated mouse ear are temporally
separate and can be differentially modulated by pharmacologic agents.
Agents Actions 1989;26(3-4):335-41.
45. Payá M, Ferrándiz ML, Sanz MJ, Bustos G, Blasco R, Rios JL, et al.
Study of the antioedema activity of some seaweed and sponge extracts
from the mediterranean coast in mice. Phytother Res 1993;7(2):159-62.
46. Zhao J, Deng JW, Chen YW, Li SP. Advanced phytochemical analysis
of herbal tea in China. J Chromatogr A 2013;1313:2-23.
47. Ubillas R, Jolad SD, Bruening RC, Kernan MR, King SR, Sesin DF,
et al. SP-303, an antiviral oligomeric proanthocyanidin from the latex
of Croton lechleri (Sangre de Drago). Phytomedicine 1994;1(8):77-106.
48. Sylvestre M, Pichette A, Longtin A, Nagau F, Legault J. Essential oil
analysis and anticancer activity of leaf essential oil of Croton flavens L.
from Guadeloupe. J Ethnopharmacol 2006;103:99-102.
49. Motta LB, Furlan Cl, Santos DY, Salatino ML, Negri G, de Carvalho JE,
et al. Antiproliferative activity and constituents of leaf extracts of Croton
sphaerogynus Baill. (Euphorbiaceae). Ind Crops Prod 2013;50:661-5.
50. Lopes MI, Saffi J, Echeverrigaray S, Henriques JA, Salvador M.
Mutagenic and antioxidant activities of Croton lechleri sap in biological
systems. J Ethnopharmacol 2004;95(2-3):437-45.
51. Cai Y, Evans F, Roberts M, Phillipson J, Zenk M, Gleba Y. Polyphenolic
compounds from Croton lechleri. Phytochemistry 1991;30(6):2033-40.
52. Chohan M, Naughton DP, Jones L, Opara EI. An investigation of the
relationship between the anti-inflammatory activity, polyphenolic
content, and antioxidant activities of cooked and in vitro digested
culinary herbs. Oxid Med Cell Longev 2012;2012:627843.
53. Diaz P, Jeong SC, Lee S, Khoo C, Koyyalamudi SR. Antioxidant and
anti-inflammatory activities of selected medicinal plants and fungi
containing phenolic and flavonoid compounds. Chin Med 2012;7(1):26.
54. Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Smith PT,
et al. Antioxidant and anti-inflammatory activities of selected medicinal
plants containing phenolic and flavonoid compounds. J Agric Food
Chem 2011;59:12361-7.
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How to Cite
Sánchez, W., L. F. Ospina, PérezC. C., W. A. Murillo, and J. J. Mendez. “PHYTOCHEMICAL SCREENING, FREE RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITY OF CROTON LEPTOSTACHYUS KUNTH LEAF EXTRACTS.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 190-4,
Original Article(s)