THE EFFECT OF CHITOSAN ON OSTEOCLAST VIABILITY, BONE RESORPTION AND RADICAL OXYGEN PRODUCTION OF PRIMARY OSTEOCLAST CULTURE OF MOUSE BONE

  • Dewi Fatma Suniarti universitas indonesia
  • Sri Angky Soekanto
  • Nurtami Soedarsono
  • Basril Abbas

Abstract

Objective: The purpose of this investigation was to determine the effect of chitosan on osteoclast cells by observing cell viability, bone resorption,
and radical oxygen species (ROS) production.
Methods: Osteoclast cells were obtained from the primary culture of bone marrow mouse. The osteoclast cells were identified by tartrate-resistant
acid phosphatase (TRAP) marker both on the cells and the culture medium. The osteoclast cell viability was observed with (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium
bromide)
tetrazolium
assay
and Bradford
assay
for
total
protein
medium
culture,
while
ROS
production
was
measured

with
malondialdehyde
(MDA)
assay.
Slices
of cow
cortical
bone
were
used as a substrate
for
osteoclastic
resorption
and concentrated
hydrochloric

acid
were
used to
activate
resorption
and pit formation
by
any
osteoclasts.
Results: Osteoclast cells were identified by TRAP marker and chitosan treated group cells showed lower optical density value compared to control
(p<0.05) on TRAP assay medium culture. Cell viability indicated lower on chitosan group than control (p<0.05). There was a qualitative difference of
the pits formed on the bone surface between the control and the chitosan group. There was a significant difference in MDA (mmol/ml) between the
control and the chitosan group with (p<0.05).
Conclusion: Based on this research, we conclude that chitosan inhibits the viability of osteoclast cells, decreases ROS production and bone resorption.
Keywords: Chitosan, Osteoclast proliferation, Radical oxygen species, Bone resorption.

Author Biography

Dewi Fatma Suniarti, universitas indonesia
oral biology

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How to Cite
Suniarti, D. F., S. A. Soekanto, N. Soedarsono, and B. Abbas. “THE EFFECT OF CHITOSAN ON OSTEOCLAST VIABILITY, BONE RESORPTION AND RADICAL OXYGEN PRODUCTION OF PRIMARY OSTEOCLAST CULTURE OF MOUSE BONE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 282-6, https://innovareacademics.in/journals/index.php/ajpcr/article/view/7400.
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Original Article(s)

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