QUANTIFICATION OF ANTIOXIDANT –PHYTOCHEMICAL STUDIES IN VITIS VINIFERA .L.VARIETIES

Authors

  • SRAVANTHI J
  • GANGADHAR RAO S

Abstract

objective: To quantify the antioxidant - Phytochemicals in Vitis vinifera varieties.
Methods: In this study, different antioxidant phytochemicals in V. vinifera varieties was determined in Thompson seedless, flame seedless, Kismis
chorni, Pusa navrang, and Rizamat by spectrophotometric method.
Results: Antioxidant is a compound that can delay or prevent the oxidation of lipids or other molecules by inhibiting the initiation or propagation
of an oxidizing chain reaction .The Quantification levels of various antioxidants in selected grape varieties which possess predominant quantities
of antioxidants are phenols, flavonols, flavonoids, ascorbic acid, anthocyanin, catalase, peroxidase, polyphenol oxidase, radical scavenging assay
(FRAP, ABTS, DPPH) respectively, phenols was recorded more in Rizamat (25.7±0.52 mg/g dry wt ), followed by FRAP radical scavenging activity
was maximum in Kismiss chorni variety (34±0.167µM/g dry wt ) among all the varieties Kismis chorni , Pusa navrang were showing maximum
antioxidant activity. Fruits have an important function in maintaining the physiological balance, optimal levels of antioxidants such as vitamin C
and phenol compounds ,Anthocyanin, , and phenol acids, being the most important for the human body .Vitamin C present in grapes, fights against
reactive oxygen species and polyphenols are associated with the prevention of diseases caused by oxidative stress. Phenolic content of these fruits
is significantly correlated with antioxidant capacity. These findings revealed that phytochemicals in the selected grapes have potent antioxidant
activities.
Conclusions: This study shows that maximum antioxidant activity levels were in Kismis chorni followe0d by other varieties. The present experiments
on Vitis varieties were designed to find out the important antioxidants and nutrient potential Antioxidants are vital substances which possess the
ability to protect the body from damage caused by free radical induced oxidative stress. From above study it is concluded that, Vitis vinifera promising
antioxidant and free radical scavenging activity. Thus can function as powerful free radical scavengers.
Keywords: Vitis vinifera, Antioxidant - Phytochemical studies, Antioxidant scavenging activity (ferric reducing antioxidant property, 2,2'-azinobis3ethylbenzthiazoline-sulphonic
acid, 2,2-Diphenyl
-2-picryl
hydrazyl),
Spectrophotometric
method.

References

Gao L, Mazza G. Characterisation, quantitation and distribution of

anthocyanins and colorless phenolics in sweet cherries. J Agric Food

Chem 1995;43:343-6.

Wang H, Cao G, Prior RL. Oxygen radical absorbing capacity of

anthocyanins. J Agric Food Chem 1997;45:304-9.

Hou DX. Potential mechanisms of cancer chemoprevention by

anthocyanins. Curr Mol Med 2003;3(2):149-59.

Cook NC, Samman S. Flavonoids: Chemistry, metabolism

cardioprotective effects and dietery sources. J Nutr Biochem

;7:66-76.

Hogan S, Zhang L, Chung H, Huang Z, Zoecklien B, Li J. Antioxidant

properties and bioactive component of Norton nine (Vitis aestivalis)

and Cabernet Franc (Vitis vinifera) wine grapes. LWT Food Sci Technol

;42:1269-74.

Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R. Analysis

and biological activities of anthocyanins. Phytochemistry

;64(5):923-33.

Miele L. Plants as bioreactors for biopharmaceuticals: Regulatory

considerations. Trends Biotechnol 1997;15(2):45-50.

Soares DG, Andreazza AC, Salvador M. Sequestering ability of

butylated hydroxytoluene, propyl gallate, resveratrol, and vitamins C

and E against ABTS, DPPH, and hydroxyl free radicals in chemical and

biological systems. J Agric Food Chem 2003;51(4):1077-80.

Berardini N, Knodler M, Schieber A, Carle R. Utilization of mango

peels as a source of pectin and polyphenolics. Innov Food Sci Emerg

Technol 2005;6:442-52.

Dimitrios B. Sources of natural phenolic antioxidants. Trends Food Sci

Technol 2006;17:505-12.

Laloraya M, Kumar GP, Laloraya MM. A possible role of superoxide

anion radical in the process of blastocyst implantation in Mus musculus.

Biochem Biophys Res Commun 1989;161(12):762-70.

Parihar MS, Dubey AK, Javeri T, Prakash P. Lipid peroxidation and

ascorbic acid status in respiratory organ of male and female fresh water

fish, Heteropneustes fossils exposed to temperature increase. Camp

Biochem Physiol 1995;112:309-13.

Sies H. Strategies of antioxidant defense. Eur J Biochem

;215(2):213-9.

Athar M. Oxidative stress and experimental carcinogenesis. Indian J

Exp Biol 2002;40(6):656-67.

Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine.

New York: Oxford University Press; 1989.

Bandyopadhyay U, Das D, Banerjee RK. Reactive oxygen species:

Oxidative damage and pathogenesis. Curr Sci 1990;77:658-66.

van Acker SA, Koymans LM, Bast A. Molecular pharmacology of

vitamin E: Structural aspects of antioxidant activity. Free Radic Biol

Med 1993;15(3):311-28.

Stadtman ER. Protein oxidation and aging. Science

;257(5074):1220-4.

Yoon GA, Yeum KJ, Cho YS, Chen CY, Tang G, Blumberg JB, et al.

Carotenoids and total phenolic contents in plant foods commonly

consumed in Korea. Nutr Res Pract 2012;6(6):481-90.

Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. J Agric

Food Chem 2003;51(3):609-14.

Chang C, Yang M, Wen HM, Chern JC. Estimation of total flavonoid

content in propolis by two complementary colorimetric methods.

J Food Drug Anal 2002;10(3):178-82.

Kumaran A, Karunakaran Joel RJ. Activity guided isolation and

identification of free radical scavenging components from an aqueous

extract of Coleus aroomaticus. Food Chem 2006;97:109-14.

Sun JS, Tsuang, YW, Chen IJ, Huang WC, Hang YS, Lu FJ. An ultra a

weak chemiluminiscence study on oxidative stress in rabbits following

acute thermal injuty. Supplementation with specific vitamin/mineral

combinations, cancer incidence, and disease specific mortality in the

general population. J Natl Cancer Inst 1998;85:1483-92.

Giusti MM, Wrolstad RE. Anthocyanins. Characterization and

measurement with UV-visible spectroscopy. In: Wrolstad RE, editor.

Current Protocols in Food Analytical Chemistry. Unit F1.2.1-13.

New York: John Wiley; 2001.

Ahmed S. Ascorbic acid, carotenoids, total phenolic content and

antioxidant activity of various genotypes of Brassica olerecea

encephala. J Med Biol Sci 2009;3:1-7.

Barber JM. Catalase and peroxidases in primary leaves during

development and senescence. Z Pfl Physiol 1980;97:135-44.

Kar M. Inorganic pyrophosphatase activity during rice leaf senescence.

Can J Bot 1975;53(5):503-10.

Beutler E. Red Cell Metabolism a Manual of Biochemical Methods.

ed., Vol. 24. New York: Grune and Stratton; 1984. p. 225-31.

Gache RN, Potlawar HD, Shegokar, Jadhave AD. Evaluation of

rd

enzymatic and non enzymatic antioxidant potential of Vitis vinifera L.

Asian J Exp Biol Sci 2010;123:45-9.

Gan RY, Kuang L, Xu XR, Zhang Y, Xia EQ, Song FL, et al.

Screening of natural antioxidants from traditional Chinese medicinal

plants associated with treatment of rheumatic disease. Molecules

;15(9):5988-97.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C.

Antioxidant activity applying an improved ABTS radical cation

decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.

Brand-Willams W, Cuvelier M, Berset C. Use of a free radical method

to evaluate antioxidant activity. Lebenson Wiss Technol 1995;28:25-30.

AL-Saikhan MS. Antioxidant activity and total phenolic in different

genotypes of Solanum tuberosum. J Food Sci 1995;2(60):341-7.

Vinson JA, Dabbagh YA, Serry MM, Jang J. Plant flavonoids, especially

tea flavonols, are powerful antioxidants using an in vitro oxidation

model for heart disease. J Agric Food Chem 1995;43:2800-2.

Stratil P, Kuban V, Fojtova J. Comparsion of the total phenolic

content and total antioxidant activity in Wines as determined by

spectrophotometric method. Czech J Food Sci 2004;26(4):242-53.

Du B, Bao-Jiang H, Peng-Bao S, Geng-Ying L, Li J, Zhu FM. Phenolic

content and antioxidant activity of wine grapes and table grapes. J Med

Plant Res 2012;6(17):3381-7.

Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. Dietary

antioxidant flavonoids and risk of coronary heart disease: The zutphen

elderly study. Lancet 1993;342(8878):1007-11.

Knekt P, Jarvinen R, Reunanen A, Maatela J. Flavonoid

intake and coronary mortality in Finland: A cohort study. BMJ

;312(7029):478-81.

Yang J, Meyers KJ, van der Heide J, Liu RH. Varietal differences in

phenolic content and antioxidant and antiproliferative activities of

onions. J Agric Food Chem 2004;52(22):6787-93.

Lopes P, Drinkine J, Saucier C, Glories Y. Determination of l-ascorbic

acid in wines by directinjection liquid chromatoraphy using a

polymericcolumn. Anal Chim Acta 2006;555:242-5.

Rani P, Unni KM, Karthikeyan J. Evaluation of antioxidant properties

of berries. Indian J Clin Biochem 2004;19(2):103-10.

Breksa AP, Takeoka GR, Hidalgo MB, Vilches A, Vasse J. Antioxidant

activity and phenolic content of 16 raisin grape (Vitis vinifera L.)

cultivars and selections. Food Chem 2010;121:740-5.

Mulero J, Pardo F, Zafrilla P. Antioxidant activity and phenolic

composition of organic and conventional grapes and wines. J Food

Compos Anal 2010;23:569-74.

Wada M, Kido H, Ohyama K, Ichibangas T, Kishikaw N, Ohba Y, et al.

Chemiluminescent screening of quenching effects of natural colorants

against reactive oxygen species evaluation of grape seed, Monascus,

gardenia and red radish extracts as multi-functional food additives.

Food Chem 2007;101:980-6.

Oktay M, Culcin I, Kufrevioglu OI. Determination of in vitro antioxidant

activity of fennel (Foeniculum vulgare) seed extracts. Lebenson Wiss

Technol 2000;36:263-71.

Bao J, Cai Y, Sun M, Wang G, Corke H. Anthocyanins, flavonols, and

free radical scavenging activity of Chinese bayberry (Myrica rubra)

extracts and their color properties and stability. J Agric Food Chem

;53(6):2327-32.

Sanchez GM, Giuliani LR, Nunez-Selles AJ, Davison GP,

Le´onfernandez, Santos-Buelga C, et al. Proanthocyanidins and

tannin like compounds nature occurrence dietery intake and effects on

nutrition and health. J Sci Food Agric 2000;80:1094-117.

Sun JS, Tsuang, YW, Chen IJ, Huang WC, Hang YS, Lu FJ. An

Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 273-279

Sravanthi and Rao

ultra a weak chemiluminiscence study on oxidative stress in rabbits

following acute thermal injuty. Supplementation with specific

vitamin/mineralcombinations, Cancer incidence, and disease specific

mortality in the general population. J Natl Cancer Inst 1998; 85:

-92.

Xu J, Guo CJ, Yang JJ, Wei JY, Li YF, Pang W, et al. Intervention of

antioxidant system function of aged rats by giving fruit juices with

different antioxidant capacities. Chin J Prev Med 2005;39:80-3.

Longanayaki N, Manian S. In vitro antioxidant properties of indigenous

underutilized fruits. Food Sci Biotechnol 2010;19:724-34.

Raghavendra M, Madhusudhana Reddy A, Yadav PR, Sudarshan

Raju A, Siva Kumar L. Comparative studies on the vitro antioxidant

properties of methanolic leafy extracts from the six edible leafy

vegetable of India. Asian J Pharm Clin Res 2013;6(3):96-9.

Published

2015-09-01

How to Cite

J, S., and G. RAO S. “QUANTIFICATION OF ANTIOXIDANT –PHYTOCHEMICAL STUDIES IN VITIS VINIFERA .L.VARIETIES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 5, Sept. 2015, pp. 295-01, https://innovareacademics.in/journals/index.php/ajpcr/article/view/7553.

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