EVALUATION OF ANTIOXIDANT AND ANTICANCER ACTIVITIES OF CIPADESSA BACCIFERA

  • Rajani Pobba
  • Rama Kotaiah M
  • Jayaveera Kn
  • Chandra Sekhar Kb

Abstract

Objective: This study was conducted to evaluate the antioxidant and anticancer activities of methanolic extract of Cipadessa baccifera leaves.
Methods: The total phenolic content in the extract was assessed by using Folin–Ciocalteu reagent. Antioxidant activity was assesses by different
methods such as phosphomolybdate assay, ferric reducing power, 1,1-Diphenyl-2-picryl-hydrazyl, superoxide scavenging assay. Cytotoxic potential
of the extract against Ehrlich Ascites carcinoma (EAC), colon carcinoma (HT-29), and breast adenocarcinoma (MCF-7) cell lines was evaluated by
3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay.
Results: The total phenolic content present in the methanolic extract of C. baccifera was found to be 338.38 mg GAL/L. In phosphomolybdate assay,
C. baccifera extract showed good antioxidant activity (half maximal inhibitory concentration [IC
]=0.42 mg/ml) which was comparable to the
standard ferulic acid (IC
50
=0.22 mg/ml). The extract showed better cytotoxicity against EAC (IC
50
50
3
=4.22 mg/ml) and HT-29 cell lines (IC
=1.86 mg/ml)
compared the MCF-7 cell lines (IC
50
=34.28 mg/ml).
Conclusion: The extract showed good radical scavenging activity and anticancer activity against EAC and HT-29 cell lines. However, the activity was
less compared to the standard. Further analysis is required to confirm the in vivo activity of the extract. The extract showed negligible cytotoxic effect
against MCF-7 cell lines.
Keywords: Anticancer, Antioxidant, Methanolic extract, Cipadessa baccifera, Radical scavenging, 1,1-Diphenyl-2-picryl-hydrazyl assay, Ehrlich Ascites
carcinoma, HT-29, MCF-7.

References

1. Santhosh Kumar S, Subramanian A, Suja SK, Sudarshan M,
Chakraborty A. Free radical scavenging capacity and antioxidant
activity of an ayurvedic medicinal plant Solanum rubrum. Res J Pharm
Biol Chem Sci 2013;4:417-26.
2. Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of
chronic disease. Crit Rev Food Sci Nutr 2004;44(4):275-95.
3. Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Bahramian F,
Bekhradnia AR. Antioxidant and free radical scavenging activity
of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C.
speciosum. Pak J Pharm Sci 2010;23(1):29-34.
4. Mangal M, Sagar P, Singh H, Raghava GP, Agarwal SM. NPACT:
Naturally occurring plant-based anti-cancer compound-activity-target
database. Nucleic Acids Res 2013;41:D1124-9.
5. Vongtau HO, Abbah J, Chindo BA, Mosugu O, Salawu AO,
Kwanashie HO, et al. Central inhibitory effects of the methanol
extract of Neorautanenia mitis root in rats and mice. J Pharm Biol
2005;43:113-20.
6. Oluyemi KA, Okwuonu UC, Baxter DG, Oyesola TO. Toxic effects
of methanolic exract of Aspilia africana leaf on the estrous cycle and
uterine tissues of Wistar rats. Int J Morphol 2007;25:609-14.
7. Wealth of India (Raw Materials). Publication and Information
Directorate. Vol. X. New Delhi: CSIR; 1976. p. 562.
8. Chetty KM, Sivaji K, Rao KT. Flowering Plants of Chittor District
Andhra Pradesh, India. 1st ed. Tirupati: Students Offset Printers;
2008. p. 64.
9. Roy A, Saraf S. Limonoids: Overview of significant bioactive triterpenes
distributed in plants kingdom. Biol Pharm Bull 2006;29(2):191-201.
10. Kindo I, Britto SJ, Arulappan MT, Thomas S, George M, Marandi R.
Evaluation of the antioxidant activity of ethanolic extracts of three
ethnomedicinal plants. Eur J Biomed Pharm Sci 2014;1(2):190-8.
11. Liang L, Zhong CC, Xiao ZY. Study on chemical constituents of
Cipadessa cinerascens. Zhongcaoyao 1991;22:6-8.
12. Luo XD, Wu SH, Ma YB, Wu DG. Components of Cipadessa baccifera.
Phytochemistry 2000;55(8):867-72.
13. Pereira C, Barreto Cleber B Jr, Kuster RM, Simas NK, Sakuragui CM,
Porzel A, et al. Flavonoids and a neolignan glucoside from Guarea
macrophylla (Meliaceae). Quím Nova 2012;35(6).
14. Fang X, Di YT, Hao XJ. The advances in the limonoid chemistry of the
meliaceae family. Curr Organic Chem 2011;15(9):1363-91.
15. Gan LS, Wang XN, Wu Y, Yue JM. Tetranortriterpenoids from
Cipadessa baccifera. J Nat Prod 2007;70(8):1344-7.
16. Siva B, Suresh G, Poornima B, Venkanna A, Babu KS, Prasad KR. et al.
Cipadessin-type limonoids from the leaves of Cipadessa baccifera.
Tetrahedron Lett 2013;54:2934-7.
17. Thirunavukarasu T, Santhana Lakshmi K, Tamilarasan M, Sivamani S,
Sangeetha D, Rajash TP. In vitro antimicrobial, antioxidant, haemolytic,
thrombolytic activities and phytochemical analysis of Cipadessa
baccifera leeeaves extracts. Int J Phytomed 2014;6(1):109-14.
18. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total
phenols and other oxidation substrates and antioxidants by means of
folin-ciocalteu reagent. Methods Enzymol 1999;299:152-78.
19. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of
antioxidant capacity through the formation of a phosphomolybdenum
complex: Specific application to the determination of vitamin E. Anal
Biochem 1999;269:337-41.
20. Oyaizu M. Studies on products of browning reactions: Antioxidative
activities of product browning reaction prepared from glucosamine. Jpn
J Nutr 1986;44:307-15.
21. Sanchen-Moreno C, Larrauri JA, Saura Calixto F. A procedure to
measure the antiradical efficiency of polyphenols. J Sci Food Agric
1998;76:270-6.
22. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid
content in mulberry and their scavenging effects on superoxide radicals.
Food Chem 1999;64:555-9.
23. Mosmann T. Rapid colorimetric assay for cellular growth and survival:
Application to proliferation and cytotoxicity assays. J Immunol
Methods 1983;65(1-2):55-63.
24. Siva B, Poornima B, Venkanna A, Prasad KR, Sridhar B, Nayak VL,
et al. Methyl angolensate and mexicanolide-type limonoids from the
seeds of Cipadessa baccifera. Phytochemistry 2014;98:174-82.
Statistics
211 Views | 415 Downloads
How to Cite
Pobba, R., R. K. M, J. Kn, and C. S. Kb. “EVALUATION OF ANTIOXIDANT AND ANTICANCER ACTIVITIES OF CIPADESSA BACCIFERA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 312-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/7700.
Section
Original Article(s)