• SUDHA SRI KESAVAN S Assistant Professor, Department of Biotechnology,Sathyabama University, chennai-119


Objectives: To isolate and screen Actinomycetes with anti bacterial and antifungal activity from garden soil samples of Sathyabama University, Chennai.

Methods: Six soil samples were collected, serially diluted and plated on starch casein agar supplemented with nalidixic acid and cyclohexamide for inhibition of bacteria and fungi, respectively. Primary screening of actinomycetes isolates was done by following cross streak method against test organisms. Submerged fermentation was followed for the production of crude antibiotics. Agar well diffusion method was done to determine the antimicrobial activity of the crude extract. The MIC also quantified for the crude extract by microtiter plate assay. The promising isolate was characterized by conventional methods.

Results: On primary screening 13 out of 22 actinomycete isolates (59%) showed potential antimicrobial activity against one or more test bacteria and/or fungus.  The isolate BN8 shows antagonistic activity against all the tested bacteria and fungi, isolates BN5 and BN16 were active against only bacteria not fungi, and Isolate BN2 was active against all tested fungi. The zone of inhibition was measured by using the crude extracts of all the four isolates. The crude extract produced by isolate BN8 showed zone on inhibition against all the tested bacterium in 100 µg/ml against Pseudomonas aeroginosa (22mm), Klebsiella pneumonia (25mm), Bacillus cereus (20mm), Staphylococcus aureus (22mm),  Escherichia coli (15mm),  Aspergillus flavus (14mm),  Aspergillus niger (20mm),   Aspergillus fumicatus (10mm) respectively. The crude extracts of isolates BN2, BN5, BN16 were not exhibited any zone of inhibition against the test microbes on agar well diffusion assay. The MIC of crude extract against Pseudomonas aeroginosa (50 μg/ml), Staphylococcus aureus(25 μg/ml), Klebsiella pneumonia (25 μg/ml) and Bacillus cereus(25 μg/ml) . The MIC value of the crude extract against the bacterium Escherichia coli was 12.5 μg/ml. The MIC of 12.5 μg/ml was observed in the crude extract against Aspergillus flavus, 25 μg/ml against Aspergillus niger, and 50 μg/ml against Aspergillus fumicatus respectively.

Conclusion: This groundwork concluded that the soil samples of Sathyabama University have actinomycetes with metabolites inhibits bacterial and fungal pathogens. One of the promising isolate was identified which active against the test bacteria and fungi. The crude metabolite exhibits very large zone of inhibition when compared to the control antibiotic. The type of antimicrobial agents produced by the isolate and the phylogenic relationship of the isolate has to be investigated as well.

Author Biography

SUDHA SRI KESAVAN S, Assistant Professor, Department of Biotechnology,Sathyabama University, chennai-119

Department of Biotechnology 

Assistant Professor


1. Talbot GH, Bradley J, Edwards JE, Gilbert D, Scheld M, Bartlett JG. Bad Bugs Need Drugs: An update on the development pipeline from the antimicrobial availability task force of the infectious diseases society of America. Clin. Infect. Dis 2006; 42: 657-668.

2. Kurtboke DI, Wildman HG. Accessing Australian biodiversity. Towards an improved detection of actinomycetes. An activity report. Actinomycetes 1998;9:1-02.

3. Takizawa M, Colwell RR, Hill RT. Isolation and diversity of actinomycetes in the chasapeake bay. Appl Environ Microbiol 1993; 59:997–1002.

4. Baltz, 2005 R. Baltz Antibiotic discovery from actinomycetes: will a renaissance follow the decline and fall. SIM News 2005: 55 (5):186–196.

5. Oskay M, Tamer AU, Azeri C. Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African Journal of Biotechnology 2004; 3 (9): 441-446.

6. Zulaybar T, Papa I, Marfori E, and Mercado B. Proceedings of the 5th Asia-Pacific Biotechnology Congress and the 35th Annual Convention of the Philippine Society for Microbiology, Inc (PSM), May 13-15, 2006, Bohol, Philippines.

7. Gurung TD, Sherpa C, Agrawal VP, Lekhak B. Isolation and characterization of antibacterial actinomycetes from soil samples of Kalapatthar, Mount Everest Region. Nepal J Sci Technol 2009; 10: 173-182.

8. Nanjwade BK, Chandrashekhara S, Shamarez AM, Goudanavar SP, Manvi VF. Isolation and morphological characterization of antibiotic producing actinomycetes. Trop J Pharma Res 2010; 9(3): 231-236.

9. Thenmozhi M, Kannabiran K. Studies on isolation, classification and phylogenetic characterization of novel antifungal Streptomyces sp. VITSTK7 in India. Curr Res J Biol Sci 2010; 2(5): 306-312.

10. Zied Zarai, Adel Kadri, Ines Ben Chobba, Riadh Ben Mansour, Ahmed Bekir, Hafedh Mejdoub, and Néji Gharsallah. L. essential oil grown in Tunisia 2010; 10(1):161.

11. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol 1966; 16: 313-340.
12. Abo-Shadi M, Sidkey NM, Al-Mutrafy AM. Antimicrobial agent producing microbes from some soils’ rhizosphere. J Am Sci 2010; 6(10): 915-925.

13. Kannan RR, Vincent SG. Molecular characterization of antagonistic streptomyces isolated from a mangrove swamp. Asian J Biotechnol 2011; 3(3): 237-243.

14. Abo-Shadi M, Sidkey NM, Al-Mutrafy AM. Antimicrobial agent producing microbes from some soils’ rhizosphere. J Am Sci 2010; 6(10): 915-925.

15. Thakur D, Yadav A, Gogoi BK, Bora TC. Isolation and screening of Streptomyces in soil of protected forest areas from the states of Assam and Tripura, India, for antimicrobial metabolites. J Med Mycol 2007; 17: 242-249.

16. Sudha sri kesavan S and Masilamani selvam M. Antibiotic compound from marine actinomycetes (Streptomyces cavourensis sp.SU 3 Nov): Isolation and purification. Asian Jr. of Microbiol. Biotech.Env.Sc 2013; 15(1):57-62.

17. Yucel S, Yemac M. Selection of Streptomyces isolates from Turkish Karstic Caves against antibiotic resistant microorganisms. Pak J Pharm Sci 2010; 23: 1-6.
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How to Cite
KESAVAN S, S. S., and H. R. “ISOLATION AND SCREENING OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM GARDEN SOIL OF SATHYABAMA UNIVERSITY, CHENNAI”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 6, Nov. 2015, pp. 110-4,
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