GC-MS ANALYSIS OF ETHANOLIC EXTRACT OF ROOTS Of RUBIA CARDIFOLIA (MANJISTHA).
Objective: The present research investigation was carried out with prime objective of studying the phytoconstituents of roots of Rubia Cardifolia after
isolation with flash chromatography technique.
Methods: The ethanolic root extract of Rubia Cardifolia was employed for GC-MS study.
Results: The study on active principles of ethanolic root extract of Rubia Cardifolia by GC-MS, showed the presence of two important compounds.
Rubiadin (30.81% area) and Purpurin (11.45 % area). More compounds would have appeared, but trying GC-MS with the isolated pure fraction by
flash chromatography of root extract lead to isolation and discovery of two principal compounds.
Conclusion: GC-MS analysis after carrying flash chromatography of ethanolic root extract led to identification of 02 pure compounds. The compounds
were found to be Rubiadin (1, 3 dihydroxy 2 methyl anthraquinone) and Purpurin (1, 2, 4 trihydroxy anthraquinone). The presence of Rubiadin and
Purpurin justifies the usage of the root extract of plant for treatment of skin ailments.
Key words: GC-MS, Rubia Cardifolia, Psoriasis, Rubiadin, Purpurin
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