KUMIS KUCING (ORTHOSIPHON STAMINEUS [BENTH.]) LEAVES ETHANOL EXTRACT AS ANTI‑OBESITY AGENT IN HIGH‑FAT DIET‑INDUCED OBESE MICE

  • Ari - Yuniarto Bandung School of Pharmacy
  • Heni Purwani
  • Dadang Juanda
  • Finna Setiawan
  • Ika Kurnia

Abstract

Objective: The leaves of kumis kucing (Orthosiphon stamineus [Benth.]) are used as folk medicine for lifestyle-related diseases such as hypertension,
hyperlipidemia, and diabetes mellitus in Southeast Asia countries, especially Indonesia. Obesity is closely associated with lifestyle-related disease.
The objective of this research was to evaluate the effect of kumis kucing leaves ethanol extract in high-fat diet-induced obese mice, and its ability to
inhibit pancreatic lipase enzyme activity in vitro.
Methods: Obese mice groups were fed a high-fat diet for 30 days and treated with kumis kucing leaves ethanol extract for 2 weeks. The measurement
parameter includes body weight, organ weight, and visceral fat mass. Experiments were also carried out to clarify the effect of kumis kucing leaves
ethanol extract inhibited pancreatic lipase enzyme activity in vitro.
Results: Kumis kucing-treated groups showed a significant decrease in body weight, organ weight, and visceral fat mass after 2 weeks treatment
compared with untreated groups. Furthermore, kumis kucing leaves ethanol extract showed potency as the pancreatic lipase activity inhibitor in vitro.
Conclusion: Kumis kucing leaves ethanol extract can inhibit the development of obesity in high-fat diet-induced obese mice. The effect appears
mediated by inhibiting the pancreatic lipase activity. The results from this study suggested that the kumis kucing leaves may have a considerable
impact as potential anti-obesity agents.
Keywords: Kumis kucing, Extract, Obesity, High-fat diet.

References

1. Yun JW. Possible anti-obesity therapeutics from nature – A review.
Phytochemistry 2010;71(14-15):1625-41.
2. Cowley P, Palmer A, Williams R. Obesity and its treatment. Drugs
Future 2008;33(12):1077-82.
3. Han LK, Zheng YN, Yoshikawa M, Okuda H, Kimura Y. Anti-obesity
effects of chikusetsusaponins isolated from Panax japonicus rhizomes.
BMC Complement Altern Med 2005;5:9.
4. Redinger RN. The pathophysiology of obesity and its clinical
manifestations. Gastroenterol Hepatol (N Y) 2007;3(11):856-63.
5. Na HN, Nam JH. Proof-of-concept for a virus-induced obesity vaccine;
vaccination against the obesity agent adenovirus 36. Int J Obes (Lond)
2014;38(11):1470-4.
6. Hill JO, Hauptman J, Anderson JW, Fujioka K, O’Neil PM, Smith DK,
et al. Orlistat, a lipase inhibitor, for weight maintenance after
conventional dieting: A 1-y study. Am J Clin Nutr 1999;69(6):1108-16.
7. Lee JK, Jang JH, Lee JT, Lee JS. Extraction and characteristics of
anti-obesity lipase inhibitor from Phellinus linteus. Mycobiology
2010;38(1):52-7.
8. Al-Yahya AA. Reproductive toxicity of Orthosiphon stamineus Benth
(Java Tea) in Swiss albino mice. Br J Pharm Toxicol 2013;4(5):181-7.
9. Son JY, Park SY, Kim JY, Won KC, Kim YD, Choi YJ, et al. Orthosiphon
stamineus reduces appetite and visceral fat in rats. J Korean Soc Appl
Biol Chem 2011;54(2):200-5.
10. Han LK, Kimura Y, Okuda H. Reduction in fat storage during chitinchitosan
treatment in
mice fed a high-fat diet. Int J Obes Relat Metab

Disord
1999;23(2):174-9.
11. Adnyana IK, Sukandar EY, Yuniarto A, Finna S. Anti-obesity effect of
the pomegranate leaves ethanol extract (Punica granatum L.) in highfat
diet induced mice. Int J Pharm Pharm Sci 2014;6(4):626-31.
12. Iswantini D, Silitonga RF, Martatilofa E, Darusman LK. Zingiber
cassumunar, Guazuma ulmifolia, and Murraya paniculata extracts as
antio-besity: In vitro inhibitory effect on pancreatic lipase. Hayati J Biol
2011;18(1):6-10.
Statistics
254 Views | 330 Downloads
How to Cite
Yuniarto, A., H. Purwani, D. Juanda, F. Setiawan, and I. Kurnia. “KUMIS KUCING (ORTHOSIPHON STAMINEUS [BENTH.]) LEAVES ETHANOL EXTRACT AS ANTI‑OBESITY AGENT IN HIGH‑FAT DIET‑INDUCED OBESE MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 6, Nov. 2015, pp. 234-6, https://innovareacademics.in/journals/index.php/ajpcr/article/view/8511.
Section
Original Article(s)

Most read articles by the same author(s)