ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF ETHANOLIC FRACTION OF ADHATODA VASICA IN A549 CELL LINE

  • Rakesh K Sindhu Chitkara College of Pharmacy Chitkara University
  • Mamta Pant
  • Sujata Basu
  • Rachana .

Abstract

Objective: To characterize and standardize the ethanolic extract of Adhatoda vasica (AV) and evaluate its antioxidant potential in vitro, and to study
its free radical scavenging effect in A549 cell line.
Methods: Antioxidant activity of AVE through DPPH, ABTS, superoxide radical scavenging activity, H2O2, NO and Lipid peroxidation assays were
carried out.
Results: In A549 cell line, the NO scavenging ability of AVE was analysed through NO production measurement. IC
values of ethanolic extract through
DPPH, ABTS, superoxide radical scavenging activity, H
2
O
2
50
, NO and lipid peroxidation assays were found to be 64, 200, 54, 62, 40 and 340 µg/ml,
respectively. The reducing power was found to increase in a concentration dependent manner. AVE (1 - 2µg/ml) scavenged NO radicals (11% and
28% in comparison to control).
Conclusion: The present study showed the ethanolic extract of AV to be a good oxygen radical scavenger and a natural source of antioxidants. AVE
showed significant nitric oxide radical scavenging activity in A549 cell line.
Keywords: Adhatoda vasica, Phytochemicals, Flavonoids, Tannins, Antioxidant potential.

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How to Cite
Sindhu, R. K., M. Pant, S. Basu, and R. . “ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF ETHANOLIC FRACTION OF ADHATODA VASICA IN A549 CELL LINE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 6, Nov. 2015, pp. 244-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/8556.
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