• Rakesh K Sindhu Chitkara College of Pharmacy Chitkara University
  • Mamta Pant
  • Sujata Basu
  • Rachana .


Objective: To characterize and standardize the ethanolic extract of Adhatoda vasica (AV) and evaluate its antioxidant potential in vitro, and to study
its free radical scavenging effect in A549 cell line.
Methods: Antioxidant activity of AVE through DPPH, ABTS, superoxide radical scavenging activity, H2O2, NO and Lipid peroxidation assays were
carried out.
Results: In A549 cell line, the NO scavenging ability of AVE was analysed through NO production measurement. IC
values of ethanolic extract through
DPPH, ABTS, superoxide radical scavenging activity, H
, NO and lipid peroxidation assays were found to be 64, 200, 54, 62, 40 and 340 µg/ml,
respectively. The reducing power was found to increase in a concentration dependent manner. AVE (1 - 2µg/ml) scavenged NO radicals (11% and
28% in comparison to control).
Conclusion: The present study showed the ethanolic extract of AV to be a good oxygen radical scavenger and a natural source of antioxidants. AVE
showed significant nitric oxide radical scavenging activity in A549 cell line.
Keywords: Adhatoda vasica, Phytochemicals, Flavonoids, Tannins, Antioxidant potential.


1. Oliveira I, Coelho V, Baltasar R, Pereira JA, Baptista P. Scavenging
capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals.
Food Chem Toxicol 2009;47(7):1507-11.
2. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free
radicals and antioxidants in normal physiological functions and human
disease. Int J Biochem Cell Biol 2007;39(1):44-84.
3. Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative
damage and oxygen deprivation stress: A review. Ann Bot
4. Aruoma OI. Assessment of potential pro-oxidant and antioxidant
actions. J Am Oil Chem Soc 1996;73:1617-24.
5. Pulido R, Bravo L, Calixto S. Antioxidant of dietary polyphenols as
determined by a modified ferric reducing antioxidant power assay.
J Agric Food Chem 2000;46:3396-402.
6. Madhavi DL, Deshpande SS, Salunkhe DK. Toxicological Aspects of
Food Antioxidants. New York: Dekker; 1995. p. 267.
7. Bursal E, Gulcin I. Polyphenolcontents and in vitro antioxidant activities
of lyophilised aqueous extract of kiwifruit (Actinidia deliciosa). Food
Res Int 2011;44:1482-9.
8. Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Bahramian F, Bekhradnia
AR. Antioxidant and free radical scavenging activity of H. officinalis
L. Var. angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak J
Pharm Sci 2010;23(1):29-34.
9. Rachana, Basu S, Pant M, Kumar MP, Saluja S. Review and future
perspectives of using vasicine and related compounds. Indo Glob J
Pharm Sci 2011;1:85-98.
10. Kirtikar KR, Basu BD, Basu LM. Indian Medicinal Plants. 2
ed., Vol.
III. Allahadad, India: Lalit Mohan Basu; 1935. p. 1593-2393.
11. Chopra RN, Nayer SL, Chopra IC. Glossary of Indian Medicinal Plants.
ed., Vol. 12. New Delhi: CSIR; 1956. p. 157.
12. Rastogi S, Mehrotra BN, Kulshreshtha DK. Proceedings of IV
International Congress of Ethnobiology. New Delhi: Deep Publications;
1994. p. 93.
13. Anonymous. Pharmacopoeia of India. 2
ed. New Delhi: Controller of
Publications, Government of India; 1966. p. 792.
14. Karthikeyan A, Shanthi V, Nagasathaya A. Preliminary phytochemical
and antibacterial screening of crude extract of the leaf of Adhatoda
vasica. L. Int J Green Pharm 2009;3:78-80.
15. Kokashi CJ, Kokashi RJ, Sharma M. Fluorescence of powdered
vegetable drugs in ultra-violet radiation. J Am Pharm Assoc
16. Liyana-Pathirana CM, Shahidi F. Antioxidant activity of commercial
soft and hard wheat (Triticum aestivum L.) as affected by gastric pH
conditions. J Agric Food Chem 2005;53(7):2433-40.
17. Brand-Williams W, Cuvelier ME, Berset C. Use of a radical
method to evaluate antioxidant activity. Lebenson Wiss Technol
18. Lai LS, Chang PC, Chang CT. Isolation and characterization of
superoxide dismutase from wheat seedlings. J Agric Food Chem
19. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and
inhibition of intercellular communication by antioxidant catechins
isolated from Chinese green tea. Carcinogenesis 1989;10(6):1003-8.
20. Garrat DC. The Quantitative Analysis of Drugs. 3
ed. Japan: Chapman
and Hall; 1964.
21. Amir M, Khan A, Mujeeb M, Ahmad A, Usmani S, Akhtar M.
Phytochemical analysis and in vitro antioxidant activity of Zingiber
officinale. Free Radic Antioxid 2011;1:75-81.
22. Sivakumar V, Rajan MS, Riyazullah MS. Preliminary phytochemical
screening and evaluation of free radical scavenging activity of
Tinospora cordifolia. Int J Pharm Pharm Sci 2010;2:186-8.
23. Kikuzaki H, Usuguchi J, Nakatani N. Constituents of Zingiberaceae
I. Diarylheptanoid from the rhizomes of ginger (Zingiber officinale
Roscoe). Chem Pharm Bull 1991;39:120-2.
24. Ottolenghi A. Interaction of ascorbic acid and mitochondria lipids. Arch
Biochem Biophys 1959;79:355-63.
25. Cushion MT, Walzer PD. Growth and serial passage of Pneumocystis
carinii in the A549 cell line. Infect Immun 1984;44(2):245-51.
26. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS,
Tannenbaum SR. Analysis of nitrate, nitrite, and [
N] nitrate in
biological fluids. Anal Biochem 1982;126(1):131-8.
Asian J Pharm Clin Res, Vol 8, Issue 6, 2015, 244-249
Rachana et al.
27. Huie RE, Padmaja S. The reaction of no with superoxide. Free Radic
Res Commun 1993;18(4):195-9.
28. Korycka-Dahl M, Richardson T. Photogeneration of superoxide anion
in serum of bovine milk and in model systems containing riboflavin and
amino acids. J Dairy Sci 1978;61:400-7.
29. Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide
anions. Biochem Pharmacol 1988;37(5):837-41.
30. Sahaa MR, Hasana SM, Aktera R, Hossaina MM, Alamb MS,
Alam MA, et al. In vitro free radical scavenging activity of methanol
extract of the leaves of Mimusops elengi Linn. Bangladesh J Vet Med
31. Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in
health and disease. Physiol Rev 2007;87(1):315-424.
32. Mamta P, Sujata B, Rachana. Anti-oxidant activity and cytoprotective
potential of ethanolic extract of Adhatoda vasica. Int J PharmTech Res
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How to Cite
Sindhu, R. K., M. Pant, S. Basu, and R. . “ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF ETHANOLIC FRACTION OF ADHATODA VASICA IN A549 CELL LINE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 6, Nov. 2015, pp. 244-9,
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